Influence of particle size of nano zinc oxide on the controlled delivery of Amoxicillin

The nucleation and growth of hydroxyapatite is closely associated with the extracellular matrix environment. Bovine Serum Albumin, Collagen and poly vinyl alcohol were used to mimic the extracellular matrix. An attempt to understand the role of these matrices on the synthesis and function of Hydroxyapatite has been made. XRD, FT-IR, XPS, TG-DTA, SEM and TEM confirmed the formation of hydroxyapatite synthesized by the biomimetic route. Further the role of organic matrix in controlling the nucleation and growth of hydroxyapatite particles in the nano level is understood by the in-depth analysis of the XPS spectra. The in vitro release of the anti-cancer drug methotrexate, in aqueous solution was studied and the in vitro release profile was assayed by elution in phosphate buffered saline with pH 7.4 and pH 5 at 37 º C. The percentage of loading and release profiles of drug were evaluated. The results show that the use of matrix increased the drug release efficiency from 44.5% to 66% at pH 7.4 and 78% to 98.92% at pH 5. These results suggest that the synthesized hydroxyapatite can be used as a pH responsive vehicle for delivering drugs. Further the release profile was predicted by Higuchi and Peppas model. The results suggested that the release mechanism was governed by the Fickian diffusion for initial 8 hrs followed by anomalous transport for longer time. The cytocompatibility of the materials was evaluated by in vitro cytotoxicity test. Both MTT and live/dead assay observations indicated that the material had no adverse impact on the cell proliferation. The results imply these composites to be bioactive having a good cytocompatibility. Although all the matrices showed good results, the one with poly vinyl alcohol exhibited higher biocompatibility and drug release efficiency. A plausible explanation was proposed for the enhanced drug delivery efficiency of these materials.

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“…makes HA a suitable candidate for carrying drugs [73][74][75][76]. makes HA a suitable candidate for carrying drugs [73][74][75][76].…”

Section: Drug Loading and Release Studymentioning

confidence: 99%

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

2016

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“…[15] In addition, zinc has shown antibacterial effects. [16] In drug release systems, AMOX has been used with different drug carriers such as zinc oxide, [17] Chitosan and poly (acrylic acid), [18] HPMC, [19] sodium alginate coated with Chitosan. [20] Amoxicillin trihydrate is semisynthetic antibiotic.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterisation and sustained release properties of layered zinc hydroxide intercalated with amoxicillin trihydrate

Nabipour

Sadr

Thomas

2015

Journal of Experimental Nanoscience

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The intercalation of amoxicillin trihydrate (AMOX) into layered zinc hydroxide (LZH) has been achieved via ion-exchange method. The resulted nanocomposite (AMOX-LZH) has been thoroughly characterised by a number of techniques including powder X-ray diffraction (PXRD), FT-IR spectroscopy, TGA/DTG analysis and scanning electron microscopy (SEM). The PXRD results showed that the gallery height of LZH was expanded from 9.57 to 11.97 A , indicating that the AMOX anions were successfully intercalated into the interlayer space of LZHs. The increased thermal stability of nanocomposite was confirmed by TGA/ DTG analyses. Improved antibacterial activity and minimum inhibitory concentration of AMOX-LZH nanocomposite was evaluated against Grampositive bacteria Staphylococcus aureus, Klebsiella pneumonia and Gram-negative Escherichia coli using the disk diffusion technique. The sustained release of amoxicillin drug from the AMOX-LZH nanocomposite was also verified.

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“…However, the bioactivity of CS is weak and hence biologically active materials like collagen or GEL are added to overcome this problem GEL with Arg–Gly–Asp (RGD)‐like sequence, encourages cell adhesion and migration Since at physiological pH, which is almost neutral, the antibacterial activity of CS is very low antibacterial agents should be added to improve the antibacterial properties of CS in pH 7.4 . It had been shown that, zinc oxide nanoparticles with a size scale of smaller than 100 nm and at the appropriate concentration have powerful antibacterial activity without adverse effect on normal cells . Application of CS accompanying with ZnO has recently gained the interest of scientists working on the area of regenerative medicine .…”

Section: Introductionmentioning

confidence: 99%

In situ synthesized chitosan–gelatin/ZnO nanocomposite scaffold with drug delivery properties: Higher antibacterial and lower cytotoxicity effects

Rakhshaei

Namazi

Hamishehkar

et al. 2019

J of Applied Polymer Sci

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In this work, chitosan–gelatin/zinc oxide nanocomposite hydrogel scaffolds (CS–GEL/nZnO) were prepared via in situ synthesis of ZnO nanoparticles (nZnO) to reach a scaffold with both inherent antibacterial and drug delivery properties. The prepared nanocomposite hydrogel scaffolds were characterized using scanning electron microscopy, transmission electron microscopy, atomic absorption spectrometer, Fourier transform infrared spectroscopy, and X‐ray diffraction. In addition, swelling, biodegradation, antibacterial, cytocompatibility, and cell attachment of the scaffolds were evaluated. The results showed that the prepared scaffolds had high porosity with a pore size of 50–400 μm and nZnO were well distributed without any agglomeration on the CS–GEL matrix. In addition, the nanocomposite scaffolds showed enhanced swelling, biodegradation, and antibacterial properties. Moreover, the drug delivery studies using naproxen showed that nZnO could control naproxen release. Cytocompatibility of the samples was proved using normal human dermal fibroblast cells (HFF2). In comparison to the previous reports which nZnO were simply added to the matrix of the scaffold, in situ synthesis of nZnO was led to higher antibacterial and lower cytotoxicity effects as a result of well distribution of nZnO in this method. According to the findings, the in situ synthesized CS–GEL/nZnO is strongly recommended for biomedical applications especially skin tissue engineering. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47590.

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Influence of particle size of nano zinc oxide on the controlled delivery of Amoxicillin

Cited by 50 publications

References 35 publications

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

Synthesis, characterisation and sustained release properties of layered zinc hydroxide intercalated with amoxicillin trihydrate

In situ synthesized chitosan–gelatin/ZnO nanocomposite scaffold with drug delivery properties: Higher antibacterial and lower cytotoxicity effects

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