Influence of NaCl and pH on lysostaphin catalytic activity, cell binding, and bacteriolytic activity

Konstantinova, Svetlana V.; Grishin, A. V.; Lyashchuk, A. M.; Vasina, I.V.; Karyagina, A. S.; Лунин, В. Г.

doi:10.1007/s00253-022-12173-w

Cited by 3 publications

(1 citation statement)

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“…The column was washed first with 0.1% Triton X-100, 50 mM NaCl, and 20 mM Tris-HCl, pH 7.5, then with 80 mM NaCl and 20 mM Tris-HCl, pH 7.5 and the recombinant protein was eluted with 180 mM NaCl and 20 mM Tris-HCl, pH 7.5. Eluted protein was dialyzed against water and treated with EDTA and ZnSO 4 as described before [ 30 ] to ensure that the active site was occupied by Zn 2+ ions. After treatment, lysostaphin was again dialyzed against water, aliquoted, and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

et al. 2023

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Background: Diopside-based ceramic is a perspective biocompatible material with numerous potential applications in the field of bone prosthetics. Implantable devices and materials are often prone to colonization and biofilm formation by pathogens such as Staphylococcus aureus, which in the case of bone grafting leads to osteomyelitis, an infectious bone and bone marrow injury. To lower the risk of bacterial colonization, implanted materials can be impregnated with antimicrobials. In this work, we loaded the antibacterial enzyme lysostaphin on diopside powder and studied the antibacterial and antibiofilm properties of such material to probe the utility of this approach for diopside-based prosthetic materials. Methods: Diopside powder was synthesized by the solid-state method, lysostaphin was loaded on diopside by adsorption, the release of lysostaphin from diopside was monitored by ELISA, and antibacterial and anti-biofilm activity was assessed by standard microbiological procedures. Results and conclusions: Lysostaphin released from diopside powder showed high antibacterial activity against planktonic bacteria and effectively destroyed 24-h staphylococcal biofilms. Diopside-based materials possess a potential for the development of antibacterial bone grafting materials.

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Section: Methodsmentioning

confidence: 99%