Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

Kudinova, A. G.; Grishin, A. V.; Грунина, Т. М.; Poponova, M. S.; Bulygina, Inna; Gromova, Maria; Choudhary, Rajan; Сенатов, Ф.С.; Karyagina, A. S.

doi:10.3390/pathogens12020177

Cited by 2 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this method, metal alkoxides have been usually used as precursors. Metal alkoxides have different hydrolysis rate and therefore, suggesting a synthesis method with repeatability is not easy 24 . Instability of most of the metal alkoxides in the air has limited their handling in the preparation procedure.…”

Section: Introductionmentioning

confidence: 99%

“…A variety of bioceramics and composites have been used as targeted drug delivery systems 21 – 23 . Kudinova et al 24 have loaded the antibacterial enzyme lysostaphin on diopside powder and studied the antibacterial and antibiofilm properties of such material to probe the utility of this approach for diopside-based prosthetic materials. Porous diopside microspheres were employed as a carrier for dexamethasone.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Low temperature preparation of diopside nanoparticles: in-vitro bioactivity and drug loading evaluation

sobhani,

Salimi

2023

Sci Rep

View full text Add to dashboard Cite

Bioactive diopside (CaMgSi2O6) nanoparticles have recently gained potential usefulness as bone replacement materials and nano vehicles for delivering therapeutics. The structural characteristics of this ceramic have found to be a key factor in bone bonding ability. To attain the desired product for 100% clinical success, it is important to realize the relationship between structure and biological activity. Synthesis of these nanoparticles via the solid-state method has been regarded as a low-cost and easy process in large-scale, but time consuming reactions and high temperature (≈ 1400 °C) are required. On the other side, the wet chemistry can overcome these drawbacks, whereas the presence of byproducts in the final powder has limited this method in large-scale production. The present document has represented a simple, fast and one-pot sol–gel approach for the synthesis of highly pure diopside nano-powders (< 20 nm) by using not-expensive precursors. Calcination of the obtained powder has been conducted at various temperatures (700, 1000 and 1200 °C). The physicochemical and microstructural properties of the products have been characterized by XRD, FTIR, FESEM and TEM. Moreover, the impact of the crystallinity on the bioactivity, drug loading capacity and drug release behavior of the synthesized nanoparticles have been investigated here for the first time. The in-vitro bioactivity results of the prepared diopside samples in a simulated body fluid (SBF) at 37 °C revealed the higher capability of the sintered sample to deposit calcium phosphate, compared with the amorphous one. High quantity of gentamicin (around 10 µg) could attach to the surface of 1 miligram of the sintered diopside during the early stages of contact (3 h), suggesting the potential use of diopside as a new class of nano-vehicles for antibiotics. The release behavior indicated a sustained release of gentamicin (80%) after 24 h. In conclusion, diopside nanoparticles can be a promising candidate as a drug-vehicle for bone filling, implant coating or bone cement applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low temperature preparation of diopside nanoparticles: in-vitro bioactivity and drug loading evaluation

sobhani,

Salimi

2023

Sci Rep

View full text Add to dashboard Cite

show abstract

Dual‐Functional Implant Based on Gellan‐Xanthan Hydrogel with Diopside, BMP‐2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection

Karyagina,

Grishin,

Kudinova

et al. 2024

Macromolecular Bioscience

View full text Add to dashboard Cite

A new dual‐functional implant based on gellan‐xanthan hydrogel with calcium‐magnesium silicate ceramic diopside and recombinant lysostaphin and bone morphogenetic protein 2 (BMP‐2)‐ray is developed. In this composite, BMP‐2 is immobilized on microparticles of diopside while lysostaphin is mixed directly into the hydrogel, providing sustained release of BMP‐2 to allow gradual bone formation and rapid release of lysostaphin to eliminate infection immediately after implantation. Introduction of diopside of up to 3% (w/v) has a negligible effect on the mechanical properties of the hydrogel but provides a high sorption capacity for BMP‐2. The hydrogels show good biocompatibility and antibacterial activity. Lysostaphin released from the implants over a 3 h period efficiently kills planktonic cells and completely destroys 24 h pre‐formed biofilms of Staphylococcus aureus. Furthermore, in vivo experiments in a mouse model of critically‐sized cranial defects infected with S. aureus show a complete lack of osteogenesis when implants contain only BMP‐2, whereas, in the presence of lysostaphin, complete closure of the defect with newly formed mineralized bone tissue is observed. Thus, the new implantable gellan‐xanthan hydrogel with diopside and recombinant lysostaphin and BMP‐2 shows both osteogenic and antibacterial properties and represents a promising material for the treatment and/or prevention of osteomyelitis after bone trauma.

show abstract

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

Cited by 2 publications

References 42 publications

Low temperature preparation of diopside nanoparticles: in-vitro bioactivity and drug loading evaluation

Low temperature preparation of diopside nanoparticles: in-vitro bioactivity and drug loading evaluation

Dual‐Functional Implant Based on Gellan‐Xanthan Hydrogel with Diopside, BMP‐2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection

Contact Info

Product

Resources

About