Antimicrobial peptides: An alternative to traditional antibiotics

Ji, Shuaiqi; An, Feiyu; Zhang, Taowei; Lou, Mengxue; Guo, Jiawei; Liu, Kexin; Zhu, Yi; Wu, Junrui; Wu, Rina

doi:10.1016/j.ejmech.2023.116072

Cited by 18 publications

(8 citation statements)

References 174 publications

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“…AMPs are proteins synthesized from genes through the standard process of protein translation by ribosomes. In contrast, NRAMPs are synthesized via multifunctional enzyme systems, such as nonribosomal peptide synthetases (NRPs) and polyketide synthases (PKs) (Ji et al, 2024 ). NRAMPs are small molecules (typically <3 kDa) that include lipopeptides, glycopeptides, and polypeptides, which have been described in Section 2.2 .…”

Section: Proteins and Peptides As Therapeutic Optionsmentioning

confidence: 99%

“…Recent studies have shown the design principles that can be applied to identify stable, active, and nontoxic AMPs for treating infections, as well as to further increase the efficiency of existing compounds (Wang, 2023 ). Most of the AMPs developed to date are natural compounds derived from animals, plants, and microorganisms (Ji et al, 2024 ). Microbial‐derived AMPs include, among others, nisin (from Streptococcus lactis ), ε‐polylysine (from Streptomyces albulus ), and pediocin PA‐1 (from Pediococcus pentosaceus ).…”

Section: Proteins and Peptides As Therapeutic Optionsmentioning

confidence: 99%

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What is the role of microbial biotechnology and genetic engineering in medicine?

Santos‐Beneit

2024

MicrobiologyOpen

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Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and therapeutic enzymes. Genetic engineering techniques, functional genomics, and synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene‐based technologies for medical applications.

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Section: Proteins and Peptides As Therapeutic Optionsmentioning

confidence: 99%

Section: Proteins and Peptides As Therapeutic Optionsmentioning

confidence: 99%

What is the role of microbial biotechnology and genetic engineering in medicine?

Santos‐Beneit

2024

MicrobiologyOpen

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“…Considering the characteristics and the mechanism of action of the AMPs that commonly target the bacterial cell membrane through multiple mechanisms and pathways, the efficacy of the treatment is increased and the development of resistance becomes unlikely to occur [24,31,[86][87][88][89]. AMPs are sequences that generally have less than 100 amino acids.…”

Section: Introductionmentioning

confidence: 99%

Bioactive peptides as alternative treatment for Helicobacter pylori infection

Franca-Oliveira,

Hernández-Ledesma,

Martinez-Rodriguez

2024

BCHD

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The infection and chronic inflammatory response generated by Helicobacter pylori is a global health concern. This pathogen is characterized as a major risk factor in the development of gastric cancer and other diseases. Conventional eradication therapies are based on antibiotic regimens and as a consequence there is an increase in antimicrobial resistance of the pathogen strains, besides other potential side effects for the host. Therefore, it is necessary to explore new alternatives. This review delves into the realm of antimicrobial peptides, exploring their efficacy against H. pylori sourced from diverse origins. Furthermore, it sheds light on food-derived peptides exhibiting remarkable biological activity. These peptides exhibit promising effects on biomarkers associated with H. pylori infection, demonstrating anti-inflammatory and antioxidant properties validated through rigorous testing in both cell and animal models. Regarding the anti-inflammatory activity, the peptide VPY derived from soybean and the peptides derived from animal sources such as meat (β-Ala-His), egg (DEDTQAMPFR, DEDTQAMPF, MLGATSL, MSYSAGF, CR, FL, HC, LL, MK) and milk (IPAV) have reported a reduction of the cytokine IL-8, biomarker directly related to H. infection. For the antioxidant activity, peptides derived from milk (EAMAPK, AVPYPQ) and from Spirulina maxima (LDAVNR, MMLDF) have reduced ROS levels and could have a positive effect on the control of H. infection. Food-derived bioactive peptides with an anti-adhesive effect were also discussed. They derive from vegetable sources (corn, pea and wheat) and are capable of interacting with the host cells, interfering the adherence of H. pylori. Food-derived bioactive peptides have potential to avoid and/or mitigate undesired outcomes of infectious diseases due to the possibility of its application in nutraceuticals and food products, resulting in a preventive approach. Keywords: Helicobacter pylori, antibiotic resistance, bioactive peptides, antimicrobial peptides

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“…Additionally, some AMPs can penetrate cell membranes to target intracellular processes, further diversifying their mechanisms and reducing the likelihood of resistance [ 18 ]. Additionally, antimicrobial peptides exhibit broad-spectrum activity against various bacteria and have lower toxicity to host cells [ 19 , 20 ]. Being natural substances, they are considered safer and more reliable.…”

Section: Introductionmentioning

confidence: 99%

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

Ran,

Shehzadi,

Liang

et al. 2024

IJMS

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Soybean phytophthora blight is a severe menace to global agriculture, causing annual losses surpassing USD 1 billion. Present crop loss mitigation strategies primarily rely on chemical pesticides and disease-resistant breeding, frequently surpassed by the pathogens’ quick adaptive evolution. In this urgent scenario, our research delves into innovative antimicrobial peptides characterized by low drug resistance and environmental friendliness. Inhibiting chitin synthase gene activity in Phytophthora sojae impairs vital functions such as growth and sporulation, presenting an effective method to reduce its pathogenic impact. In our study, we screened 16 previously tested peptides to evaluate their antimicrobial effects against Phytophthora using structure-guided drug design, which involves molecular docking, saturation mutagenesis, molecular dynamics, and toxicity prediction. The in silico analysis identified AMP_04 with potential inhibitory activity against Phytophthora sojae’s chitin synthase. Through three rounds of saturation mutagenesis, we pin-pointed the most effective triple mutant, TP (D10K, G11I, S14L). Molecular dynamic simulations revealed TP’s stability in the chitin synthase-TP complex and its transmembrane mechanism, employing an all-atom force field. Our findings demonstrate the efficacy of TP in occupying the substrate-binding pocket and translocation catalytic channel. Effective inhibition of the chitin synthase enzyme can be achieved. Specifically, the triple mutant demonstrates enhanced antimicrobial potency and decreased toxicity relative to the wild-type AMP_04, utilizing a mechanism akin to the barrel-stave model during membrane translocation. Collectively, our study provides a new strategy that could be used as a potent antimicrobial agent in combatting soybean blight, contributing to sustainable agricultural practices.

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Antimicrobial peptides: An alternative to traditional antibiotics

Cited by 18 publications

References 174 publications

What is the role of microbial biotechnology and genetic engineering in medicine?

What is the role of microbial biotechnology and genetic engineering in medicine?

Bioactive peptides as alternative treatment for Helicobacter pylori infection

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

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