Influence of medium constituents on the biosynthesis of cephalosporin-C

Nigan, Vinod Kumar; Verma, Ruchi; Kumar, Abhishek; Kundu, Subrata; Ghosh, Purnendu

doi:10.2225/vol10-issue2-fulltext-8

Cited by 11 publications

(12 citation statements)

References 7 publications

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“…The reduction in CPC yield was due to accumulation of sucrose in broth at higher concentrations, inhibiting the synthesis of enzyme responsible for production of CPC. The results obtained are incompliance with findings of Nigam et al 2007.…”

Section: Effect Of Different Sucrose Concentrations On Cpc Biosynthescontrasting

confidence: 94%

“…Enzymatic and genetic studies on methionine have revealed that it induces four of the enzymes of CPC biosynthesis at the level of transcription. It was also converted to cystine, on of the three precursors of CPC (Nigam et al, 2007).…”

Section: Effect Of Different Dl-methionine Concentrations On Cpc Biosmentioning

confidence: 99%

“…In the international market Cephalosporins have been proved as broad spectrum antibiotics belonging to β-lactams along with the penicillin but more resistant to β-lactamases than later (Kawahara et al, 2015). Similar to the penicillin G, cephalosporin-C (CPC) is also produced during aerobic fermentations, acting as starting material for the manufacture of various semisynthetic cephalosporins by modifications on its side chains (Nigam et al, 2007). Different species of Acremonium have been investigated as potential source of cephalosporins.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

Bilal¹,

Yousaf²,

Siddique³

et al. 2016

IJEAB

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Section: Effect Of Different Sucrose Concentrations On Cpc Biosynthescontrasting

confidence: 94%

Section: Effect Of Different Dl-methionine Concentrations On Cpc Biosmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

Bilal¹,

Yousaf²,

Siddique³

et al. 2016

IJEAB

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“…The benefits of in vitro cultivation include the ability to manipulate the culture medium to optimise mycelia growth and a shorter cultivation time (Paterson, 2006). It is known that media composition and their constituent's concentrations also influence fungi and plant cells metabolism and production of metabolites in vitro (Chattopadhyay, Farkya, Srivastava, & Bisaria, 2002;Nigam, Verma, Kumar, Kundu, & Ghosh, 2007). The production of fruiting bodies includes a long cultivation in a plastic bag and, therefore, mycelia are alternative or substitute products of mature fruiting bodies, for use in the formulation of nutraceuticals and functional foods (Saltarelli et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

Heleno

Barros

Martins³

et al. 2012

Food Research International

136

107

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Abbreviations CATCatalase DAD Diode array detector DPPH 2,2-Diphenyl-1-picrylhydrazyl EC 50Extract concentration providing 50% antioxidant activity or 0.5 absorbance in the reducing power assay FB Fruiting body GAE Gallic acid equivalents GPx Glutathione peroxidase GSH Reduced glutathioneHPLC High-performance liquid chromatography IS Internal standard M Mycelium MMN Melin-Norkans medium MS Mass spectrometry PDA Potato dextrose agar medium PE Polysaccharides equivalents Ph Phenolic extract Ps Polysaccharidic extract RI Refraction index RSA Radical scavenging activity S Spores SOD Superoxide dismutaseTBARS Thiobarbituric acid reactive substances v/v ml/100 ml w/v g/100 ml ABSTRACTGanoderma lucidum is one of the most extensively studied mushrooms due to its medicinal properties. Herein, a systematic study was carried out in order to compare the antioxidant activity of phenolic and polysaccharidic extracts from fruiting body, spores and mycelium, obtained in three different culture media, of G. lucidum from NortheastPortugal. Phenolic extracts were characterized using high-performance liquid chromatography coupled to photodiode array detection, while polysaccharidic extracts were hydrolysed and further characterized using HPLC and refraction index detection.In general, the phenolic extracts (Ph) proved to have higher antioxidant potential than their corresponding polysaccharidic extracts (Ps). Amongst phenolic extracts, FB-Ph provided the highest antioxidant activity (EC 50 ≤ 0.6 mg/ml) and the highest content in total phenolics (~29 mg GAE/g extract) and phenolic acids (p-hydroxybenzoic and pcoumaric acids). S-Ps was the polysaccharidic extract with the best antioxidant activity (EC 50 ≤ 2 mg/ml); nevertheless, the highest levels of total phenolics were obtained in FB-PS (~56 mg GAE/g extract), while the highest levels of total polysaccharides (~14 mg PE/g extract) and individual sugars were observed in mycelia obtained from solid culture media, M-PDA-Ps and M-sMMN-Ps. The free radical scavenging properties, reducing power and lipid peroxidation inhibition of G. lucidum seemed to be correlated with phenolic compounds mostly in a free form, but also linked to polysaccharides.

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“…Penentuan konsentrasi terbaik dari sumber karbon terpilih mengacu pada penelitian Nigam et al (2007). Level konsentrasi molases yang diuji dalam media kultivasi antara lain 10,20,30,40,50,60,70,80, dan 90 g/L dengan total karbon yang diuji pada masing-masing konsentrasi adalah 9,6; 19,2; 28,8; 38,8; 48; 57,6; 67,2; 76,8; 86,4% karbon.…”

Section: Penentuan Konsentrasi Sumber Karbonunclassified

PENINGKATAN PRODUKSI SEFALOSPORIN C DARI <i>Acremonium chrysogenum</i> CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN

Prabandari

Hidayati

Dewi

et al. 2017

J Bioteknol Biosains Indones

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Cephalosporin is a β-lactam antibiotic produced by Keywords:Carbon, cephalosporin C, cultivation medium, nitrogen, A. chrysogenum ABSTRAK Sefalosporin C adalah golongan antibiotik β-lactam yang dihasilkan Acremonium chrysogenum melalui fermentasi cair. Komponen yang sangat berpengaruh terhadap produksi sefalosporin C adalah sumber karbon dan nitrogen. Penelitian ini bertujuan mendapatkan komposisi media terbaik untuk produksi sefalosporin C. Optimasi dilakukan menggunakan metode respon permukaan. Hasil menunjukkan bahwa molases 70 g/L adalah sumber karbon terbaik dan kombinasi corn steep liquor, urea dan ammonium sulfat adalah sumber nitrogen terbaik. DL-methionin, sumber karbon, dan nitrogen berpengaruh nyata terhadap produksi sefalosporin C. Optimasi menggunakan model matematika menunjukkan produksi sefalosporin C tertinggi (3876 mg/L) yang diperoleh dengan komposisi media 68,28 g/L molases, 71,61 g/L nitrogen, dan 0,4 g/L DL-methionin. Verfikasi di laboratorium menggunakan komposisi media yang sama menghasilkan sefalosporin C sebesar 3696 mg/L, berbeda 4,65% dibanding dengan hasil optimasi matematis. Optimasi media mampu meningkatkan produksi sefalosprin C sebesar 1,48 kali dibanding media yang digunakan sebelumnya, dimana maksimal hanya menghasilkan 2487 mg/L.

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Influence of medium constituents on the biosynthesis of cephalosporin-C

Cited by 11 publications

References 7 publications

Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

PENINGKATAN PRODUKSI SEFALOSPORIN C DARI <i>Acremonium chrysogenum</i> CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN

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