Influence of Linear Energy Transfer on the Radioresistance of Budding Haploid Yeast Cells

Raju, M. R.; Gnanapurani, M.; Stackler, B.; Madhvanath, U.; Howard, J.; Lyman, John; Manney, Thomas R.; Tobias, Cornelius A.

doi:10.2307/3573612

Cited by 12 publications

(6 citation statements)

References 10 publications

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“…In multiple studies, budding yeast cells have been observed to be more resistant to radiation inactivation than nonbudding yeast (23, 24, 33). Generally, exponentially growing yeast cells are more resistant to radiation cell killing than cells in stationary phase or G 1 (34).…”

Section: Discussionmentioning

confidence: 99%

“…Generally, exponentially growing yeast cells are more resistant to radiation cell killing than cells in stationary phase or G 1 (34). Similarly, medium-starved cells (presumed to be in G 0 or G 1 ) are more sensitive to radiation inactivation than nonstarved yeast (33, 35). Here yeast cells in late exponential growth are most resistant to radiation cell killing, supporting the observations of Tippins and Parry (34).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, cells in late stationary phase were most sensitive to radiation cell killing, paralleling the heightened radio-sensitivity of medium-starved yeast observed by Raju et al . (33) and Laser (35). …”

Section: Discussionmentioning

confidence: 99%

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Cell Cycle Dependence of Ionizing Radiation-Induced DNA Deletions and Antioxidant Radioprotection inSaccharomyces cerevisiae

Hafer¹,

Rivina²,

Schiestl³

2010

Radiation Research

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The yeast DEL assay is an effective method for measuring intrachromosomal recombination events resulting in DNA deletions that when occurring in mammalian cells are often associated with genomic instability and carcinogenesis. Here we used the DEL assay to measure γ-ray-induced DNA deletions throughout different phases of yeast culture growth. Whereas yeast survival differed by only up to twofold throughout the yeast growth phase, proliferating cells in lag and early exponential growth phases were tenfold more sensitive to ionizing radiation-induced DNA deletions than cells in stationary phase. Radiation-induced DNA deletion potential was found to correlate directly with the fraction of cells in S/G2 phase. The ability of the antioxidants l-ascorbic acid and DMSO to protect against radiation-induced DNA deletions was also measured within the different phases of yeast culture growth. Yeast cells in lag and early exponential growth phases were uniquely protected by antioxidant treatment, whereas nondividing cells in stationary phase could not be protected against the induction of DNA deletions. These results are compared with those from mammalian cell studies, and the implications for radiation-induced carcinogenesis and radioprotection are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cell Cycle Dependence of Ionizing Radiation-Induced DNA Deletions and Antioxidant Radioprotection inSaccharomyces cerevisiae

Hafer¹,

Rivina²,

Schiestl³

2010

Radiation Research

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“…The optimal parameters obtained by computer least squares procedures are shown in Table 2, for the cell lines listed in Table 1, with the exception of Artemia eggs (Easter and Hutchinson 1961) and budding yeast (Raju, Gnanapurani, Stackler, Madhvanath, Howard, Lyman, Manney, and Tobias 1972) for which the computer program did not converge. Since the parameters for mouse bone marrow (Broerse, Engels, LeLieveld, van Putten, Duncan, Greene, Massey, Gilbert, Hendry, and Howard 1971) and anoxically irradiated HeLa cells (Nias, Greene, Fox and Thomas 1967) were determined from X-ray and 14 MeV neutron data, these cases were not considered in this study.…”

Section: Resultsmentioning

confidence: 99%

Systematic evaluation of cellular radiosensitivity parameters

Roth

Sharma

Katz

1976

pmb

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“…Elkind and Beam (1955) first showed that in haploid yeast the resistance ratio of budding to non-budding cells was higher for X-rays than for 210 Po alpha-rays. Raju, Gnanapurani, Stackler, Madhvanath, Howard, Lyman, Manney and Tobias (1972) have repeated this investigation using heavy ions covering a wide range of LET . They have shown that the relative resistance of budding cells decreases rapidly with increasing LET attaining a constant value for radiations of LET greater than 200 keV /µm .…”

Section: Introductionmentioning

confidence: 99%