Influence of gelling agents on shoot multiplication and the uptake of macroelements by in vitro culture of rose, cordyline and homalomena

Podwyszyńska, Małgorzata; Olszewski, T.

doi:10.1016/0304-4238(95)00826-0

Cited by 17 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For these reasons and considering the high price of tissue culture grade agar, attempts have been made to identify suitable alternatives. A wide range of substances, Gelrite ® (Podwyszynska and Olszewski 1995), starch (Zimmerman et al 1995, Smýkalová et al 2001, sago (Naik and Sarkar 2001), gum katira (Jain and Babbar 2002) and xyloglucan (Lima-Nishimura et al 2003), has been tried as substitute or partial substitute for agar.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

Agar/galactomannan gels applied to shoot regeneration from tobacco leaves

Lucyszyn¹,

Quoirin²,

Homma³

et al. 2007

Biol. plant.

View full text Add to dashboard Cite

This study concerns the efficacy of partial agar substitution by galactomannans as support in plant regeneration media for Nicotiana tabacum. The production of multiple shoots from leaf-derived callus and their rooting were evaluated. The galactomannans applied were obtained from Cassia fastuosa (cassia) and Cyamopsis tetragonolobus (guar gum -a commercial galactomannan) seeds. The results obtained on media solidified with mixtures of agar/galactomannan (3 g dm -3 each) gels were compared with those on media gelled with a standard concentration of agar (6 g dm -3 ). The in vitro performance allowed to conclude that the use of galactomannans raised the number of shoots and improved their quality. Furthermore, the length of roots and the size of leaves were significantly higher in the media solidified with agar/guar galactomannan mixtures.

show abstract

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

Agar/galactomannan gels applied to shoot regeneration from tobacco leaves

Lucyszyn¹,

Quoirin²,

Homma³

et al. 2007

Biol. plant.

View full text Add to dashboard Cite

show abstract

“…Ca 2+ alone or combined with Mg 2+ at raised concentration reduced shoot-tip necrosis in microcuttings treated with IAA (Table 3). Previous study showed that raising Ca 2+ and Mg 2+ in the medium also enhanced the level of these ions in rose shoots multiplied in vitro (Podwyszyfiska and Olszewski 1995). An enhanced antisenescence effect was achieved when Ca 2+ as Ca(NO3) 2 at raised level was applied in the multiplication stage.…”

Section: Resultsmentioning

confidence: 92%

Effect of inhibitors of ethylene biosynthesis and action, as well as calcium and magnesium on rose shoot rooting, shoot-tip necrosis and leaf senescence in vitro

Podwyszyńska

Goszczynska

1998

Acta Physiol Plant

View full text Add to dashboard Cite

Microcuttings of easy-to-root dwarf rose cv. Starina, showing early symptoms of leaf senescence and shoot-tip necrosis in rooting stage, were chosen for the study. The effects of inhibitors of ethylene biosynthesis (AOA, A1B) and action (AgNO3), 2+ 2+ and Ca and Mg were studied in relation to rooting, leaf senescence and shoot-tip necrosis. The effects of these substances were examined with respect to IAA presence in a medium, which stimulated leaf yellowing and shoot-tip necrosis. AOA strongly inhibited rooting of microcuttings, but did not affect ethylene biosynthesis. AIB at 250 mg.l l and AgNO32.5 mg.1 1 in the presence of IAA did not affect rooting but effectively prevented leaf senescence. Ca 2+ alone or combined with 2+ Mg at raised concentration, or an ethylene action inhibitor Ag +, reduced shoot-tip necrosis in microcuttings treated with IAA. Addition of Ag + to IAA medium drastically increased ethylene production by the shoots. Interaction between endogenous levels of auxin, ethylene and calcium in relation to rooting, shoot-tip necrosis and leaf senescence was discussed. Ethylene could enhance tissue sensitivity to auxin. Moreover, the tissue of rose shoots is very sensitive in the in vitro condition on standard medium because of the calcium deficiency. Thus, the raised Ca/Mg level counteracted shoot-tip necrosis through enhancing cell membrane and wall resistance to ethylene and IAA.

show abstract

“…Some genotypes also contain bioactive and pro-health compounds in fruits, flower petals, and leaves; therefore, they are used as raw materials in food and herbal industries. In vitro propagation method has been developed in Poland for different rose genotypes, including miniature roses 'Starina' and 'White Gem' (Podwyszyńska & Goszczyńska, 1998;Podwyszyńska & Olszewski, 1995); the naturally occurring wild species in Poland, viz. R. canina, R. dumalis, R. rubiginoisa, and R. tomentosa (Pawłowska, 2011); rootstocks R. multiflora, R. indica, and R. manetti (Kucharska et al, 2006); as well as hiproses R. pomifera 'Karpatia' (Kwaśniewska et al, 2017) and rose 'Konstancin' (Wojtania & Matysiak, 2018).…”

Section: Rosa Sppmentioning

confidence: 99%

“…Most rose genotypes showed the best growth on MS medium supplemented with BAP alone (Kucharska et al, 2006) or in combination with gibberellic acid (GA 3 ) (Pawłowska & Szewczyk-Taranek, 2014;Wojtania & Matysiak, 2018). Podwyszyńska and Olszewski (1995) modified the mineral composition of standard MS medium by adding 1.5-fold higher levels of Ca 2+ and Mg 2+ , and twofold higher levels of Fe 2+ and Mn 2+ . is resulted in the elimination of shoot tip necrosis and leaf yellowing.…”

Section: Rosa Sppmentioning

confidence: 99%

Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

et al. 2022

View full text Add to dashboard Cite

This work focuses on the achievements of Polish researchers in the field of vegetative reproduction of plants under in vitro conditions. For more than 50 years, micropropagation methods have been used in Poland whenever vegetative reproduction is necessary. Most perennial horticultural crops, such as fruit plants, the majority of ornamental geophytes, and some vegetables (e.g., rhubarb and horseradish), require clonal reproduction owing to their high heterozygosity, i.e., their offsprings when reproduced from seeds, do not repeat the parental characteristics. Various goals have been pursued in the development of regenerative and in vitro propagation systems for each of the aforementioned groups of plants, such as pathogen elimination, reproduction of healthy plants, rapid multiplication of newly obtained valuable breeding lines and cultivars, and breeding to obtain polyploids, haploids, and doubled haploids. Owing to the growing interest of researchers and plant producers in environmentally friendly technologies, one of the sections is devoted to the issue of biotization of micropropagated plants.

show abstract

Influence of gelling agents on shoot multiplication and the uptake of macroelements by in vitro culture of rose, cordyline and homalomena

Cited by 17 publications

References 18 publications

Agar/galactomannan gels applied to shoot regeneration from tobacco leaves

Agar/galactomannan gels applied to shoot regeneration from tobacco leaves

Effect of inhibitors of ethylene biosynthesis and action, as well as calcium and magnesium on rose shoot rooting, shoot-tip necrosis and leaf senescence in vitro

Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

Contact Info

Product

Resources

About