Influence of forskolin supplementation on embryos produced in vitro

The role of forskolin as a lipolytic stimulator during in vitro oocyte maturation and the in vitro embryo production of livestock

“…(2017) found that the presence of forskolin in in vitro culture medium (5 µM) resulted in lower intracytoplasmic lipid content in blastocysts compared with the control group. On the other hand, the embryo lipid content was parallel ( p > .05) between the control group (883 ± 5.2) and forskolin treatment (772 ± 4.8) (Costa et al., 2019). The authors found that the sterol regulatory element‐binding protein 1 ( SREBF1 ) gene which has a critical role in cholesterol metabolism gene displayed no change in the levels of transcripts as response to forskolin supplementation.…”

“…However, in another study, no significant alterations were observed in the bovine cleavage rate of oocytes treated with forskolin prior to in vitro maturation (Razza, 2018). It was noticed that an inferior number of cattle embryos hatched on day 10 in the control group (48.4%) than in the forskolin‐treated group (62.9%) (Costa et al., 2019).…”

“…Forskolin has greater potential to decrease the apoptosis degree in fresh embryos than in vitrified ones at different concentrations (2.5, 5 and 10 µM) (Paschoal et al., 2017). A sensible connection between the lipid ingathering and the rate of apoptosis was documented, where forskolin at a low dose (2.5 µM) led to a superior lipolytic vestige without triggering apoptotic impairment in post‐warming embryos (Costa et al., 2019).…”

“…However, the high intracellular lipid accumulation of oocytes and embryos has been associated with decreased the oocyte developmental competence and reduced cryotolerance (Bahmanpour et al., 2020; Bilodeau‐Goeseels, 2006). In the last decades, scientists focussed to use various approaches in manipulating and altering the lipid content and structure of oocyte or embryo (Costa et al., 2019; Cuello et al., 2013).…”

“…For this fact, many studies have used in culture media to decrease lipid content by chemical lipolysis, and enhance the cryotolerance and post‐vitrification quality of embryos (Bilodeau‐Goeseels, 2011; Paschoal et al., 2014, 2016; Zeng et al., 2014). Thus, forskolin increases the intracellular concentrations of cAMP, which inhibits the immediate resumption of meiosis and gives the oocyte more time to gain optimal developmental competence (Costa et al., 2019; Razza, 2018; Richani et al., 2019).…”