Influence of Enzymatic Degradation on Physical Properties of Poly(<i>ε</i>‐caprolactone) Films and Sponges

Vidaurre, Ana; Dueñas, José María Meseguer; Estellés, Jorge Más; Cortázar, Isabel Castilla

doi:10.1002/masy.200850907

Cited by 21 publications

(18 citation statements)

References 37 publications

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“…But, on the other hand, degradation produces mass loss and porosity in the sample, resulting in a decrease of Young's modulus [34]. The porosity can be produced inside the sample, if bulk degradation occurs, or near the surface of the sample, if superficial erosion is the degradation mechanism [25]. In both cases, porosity produces deformation between the first and third compression scans, as can be seen in Table 1.…”

Section: Mechanical Testingmentioning

confidence: 99%

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Castilla-Cortázar¹,

Más-Estellés²,

Dueñas³

et al. 2012

Polymer Degradation and Stability

144

116

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Long-term hydrolytic and enzymatic degradation profiles of poly(ε-caprolactone) (PCL) networks were obtained. The hydrolytic degradation studies were performed in water and phosphate buffer solution (PBS) for 65 weeks. In this case, the degradation rate of PCL networks was faster than previous results in the literature on linear PCL, reaching a weight loss of around 20% in 60 weeks after immersing the samples either in water or PBS conditions. The enzymatic degradation rate in Pseudomonas Lipase for 14 weeks was also studied, with the conclusion being that the degradation profile of PCL networks is lower than for linear PCL, also reaching a 20% weight loss. The weight lost, degree of swelling, and calorimetric and mechanical properties as a function of degradation time were obtained. Furthermore, the morphological changes in the samples were studied carefully through electron microscopy and crystal size through X-ray diffraction. The changes in some properties over the degradation period such as crystallinity, crystal size and Young's modulus were smaller in the case of enzymatic studies, highlighting differences in the degradation mechanism in the two studies, hydrolytic and enzymatic.

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Section: Mechanical Testingmentioning

confidence: 99%

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Castilla-Cortázar¹,

Más-Estellés²,

Dueñas³

et al. 2012

Polymer Degradation and Stability

144

116

View full text Add to dashboard Cite

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“…Both polycaprolactone (PCL) and poly(L-lactide) (PLLA) are hydrophobous semicrystalline polymers. The ester groups of the main chain are susceptible to hydrolysis and thus PCL and PLLA degrades in the presence of water, phosphate-buffered saline (PBS) or lipoprotein lipase [6,7]. Other hydrolytic enzymes do not show significant effect on the degradation rate of these polymers [7e9].…”

Section: Introductionmentioning

confidence: 99%

“…The degradation rate of polymers is highly dependent of the effective surface to volume ratio, being faster in porous sponges than in thin non-porous films and faster in the later than in bulk samples [6]. It is problematic to compare the in vitro and in vivo enzymatic degradation due to the difficulty to reproduce the complex environment around the implant in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action

Fernández

Oberti

Vikingsson

et al. 2016

Polymer Degradation and Stability

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a b s t r a c tThe aim of this study is to show that introducing a small fraction of hydrophilic groups into a hydrophobic polyester favor the macrophage activity by accelerating the degradation action in aqueous media. It is also seen that differentiation of MSCs cultured in monolayer towards bone in specific differentiation media is favored in these materials with respect to the corresponding pristine polyesters. Polymer networks based in polycarpolactone or poly(L-lactide) and containing a small fraction of poly(-hydroxyethyl acrylate) have been synthesized. Degradation kinetics in vitro was monitored by mass loss and swelling capacity of the polymer network in good solvents, the later as representative of chain cleavage. Hydrolytic and enzymatic degradation is accelerated by the inclusion of poly(hydroxyethyl acrylate) blocks in the network. Macrophages were cultured on the surface of the network films, showing its capacity to erode the material surface but also to accelerate bulk degradation. Bone marrow mesenchymal stem cells were cultured in monolayer on the membranes in osteogenic media, showing an increase of specific markers expression in comparison to pristine polyesters.

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“…Os filmes colocados em coluna de solo por sistema de umidade por capilaridade ficaram mais deteriorados, e com grandes orifícios em toda sua extensão. Este tipo de efeito foi observado anteriormente, em filmes de PCL incubados em meio contendo enzimas lipase de Pseudomonas, a 37 °C por Vidaurre et al [19] , que também concluíram que a degradação enzimática do PCL não alterou sua cristalinidade, devido a biodegradação ocorrer tanto na fase amorfa como na fase cristalina. Além disso, a fase lamelar sofreu modificação, como conseqüência da fragmentação da cadeia, causada pela diminuição da massa molar das cadeias poliméricas [19] .…”

Section: Resultsunclassified

“…Este tipo de efeito foi observado anteriormente, em filmes de PCL incubados em meio contendo enzimas lipase de Pseudomonas, a 37 °C por Vidaurre et al [19] , que também concluíram que a degradação enzimática do PCL não alterou sua cristalinidade, devido a biodegradação ocorrer tanto na fase amorfa como na fase cristalina. Além disso, a fase lamelar sofreu modificação, como conseqüência da fragmentação da cadeia, causada pela diminuição da massa molar das cadeias poliméricas [19] . Os resultados mostraram que a coluna de solo, construída em garrafa PET e umedecida por capilaridade foi mais eficiente na biodegradação de filmes de PCL durante 60 dias do experimento.…”

Section: Resultsunclassified

Influência da geometria e umidade de colunas de solo na biodegradação de filmes de PCL

2011

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Resumo: Filmes de poli(ε-caprolactona) (PCL) foram preparados por compressão a quente e investigados com respeito a sua biodegradação em diferentes colunas de solo, após 60 dias. A morfologia e estrutura dos filmes, após os diferentes tratamentos, foram verificadas através de medidas de perda de massa, espectroscopia no infravermelho com transformada de Fourier (FTIR), calorimetria exploratória diferencial (DSC), termogravimetria (TG) e microscopia eletrônica de varredura (MEV). Os filmes de PCL sofreram biodegradação, principalmente na fase amorfa, atribuídas a quebras das ligações ésteres da cadeia polimérica, devido à ação de exo-enzimas dos microrganismos do solo. Os fatores abióticos, tais como temperatura, pH, quantidade de oxigênio e principalmente tipo de coluna de solo e umidade, foram aspectos considerados relevantes no processo de biodegradação dos filmes de PCL em solo. Palavras-chave: Poli(ε-caprolactona), solo, biodegradação, microrganismos. Influence of Geometry and Humidity of Soil Columns in the Biodegradation of PCL FilmsAbstract: Poly (ε-caprolactone) (PCL) films were prepared by melt-pressing and investigated with respect to their biodegradation in different soil columns after 60 days. The morphology and structure of the films, after various microbial treatments, were studied with measurements of mass loss, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetry ( TG) and scanning electron microscopy (SEM). The PCL films underwent biodegradation, mainly in the amorphous phase, attributed to breaking of ester bonds of the polymer chain due to the action of exo-enzymes from soil microorganisms. The abiotic factors such as temperature, pH, oxygen content and principally soil column type and humidity were considered important aspects for the biodegradation process on the PCL films in soil. Keywords: Poly(ε-caprolactone), soil, biodegradation, microorganisms. IntroduçãoMicrorganismos, como bactérias e fungos estão envolvidos na degradação de plásticos naturais e sintéticos [1] . A biodegradação de materiais plásticos ocorre ativamente sob diferentes condições de solo de acordo com suas propriedades, pois os microrganismos responsáveis pela degradação diferem uns dos outros pela morfologia, capacidade metabólica e condições ótimas de crescimento. A biodegradação é regida por diferentes fatores, que incluem as características do polímero, tipo de organismo, e a natureza do pré-tratamento. Os polímeros são substratos potenciais para a ação de microrganismos heterotróficos [2] .Algumas características polímericas, tais como a mobilidade, taticidade, cristalinidade, massa molar e o tipo de grupos funcionais e substituintes presentes na sua estrutura, desempenham um papel importante na sua degradação [3,4] . Durante a degradação o polímero é primeiramente convertido em seus monômeros, que posteriormente, mas não necessariamente podem ser mineralizados. A maioria dos polímeros é constituída por moléculas longas para atravessar as membranas celulares, por ...

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Influence of Enzymatic Degradation on Physical Properties of Poly(ε‐caprolactone) Films and Sponges

Cited by 21 publications

References 37 publications

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network

Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action

Influência da geometria e umidade de colunas de solo na biodegradação de filmes de PCL

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