Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action

Fernández, Juan Manuel; Oberti, Tamara G.; Vikingsson, L.; Ribelles, J.L. Gómez; Cortizo, Ana Marı́a

doi:10.1016/j.polymdegradstab.2016.05.020

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…The electrospun gelatin/chitosan nanofibrous (7:3) with appropriate mechanical strength and biodegradation can accelerate more ADSC adhesion and proliferation. Studies indicated that culture surface with higher stiffness favored cell adhesion and spreading [29,30], and hydrophilic surfaces favored BMSC spreading [31], which are consistent with our observation. The electrospun gelatin/chitosan nanofibrous was shown to not be toxic at all compositions.…”

Section: Discussionsupporting

confidence: 92%

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Zhu

Liao

Zhang

et al. 2020

Preprint

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Background Development of natural biodegradable electrospun nanofibrous with appropriate physical properties and biocompatibility is highly desirable to support multi-layer cell sheets construction for wound healing. Results We developed a series of electrospun gelatin/chitosan nanofibrous with different gelatin/chitosan ratios and controlled pore sizes, and impregnated plasmid VEGF into membrane, which as supporting membrane to construct sandwich-like adipose-derived stem cells (ADSCs) cell sheets with a simple and effective technique for accelerated wound healing. We found that the physical properties of the electrospun nanofibrous including water retention, stiffness, strength, elasticity and degradation could be tailored by changing the proportion of gelatin/chitosan. We further observed that the optimized electrospun nanofibrous with the optimal ratio of gelatin to chitosan (7:3) which were soft and elastic could most effectively support cell adhesion, proliferation and migration into the whole nanofibrous membranes. Nanofibrous delivered plasmid VEGF facilitating multi-layer ADSC cell sheets formation and promoting regeneration of cutaneous tissues within two weeks. Conclusions Such natural biodegradable and biocompatible electrospun gelatin/chitosan nanofibrous with plasmid have the potential to become fully cellularized and support sandwich-like ADSC cell sheets formation, which will make it suitable for widespread applications such as skin substitute or wound dressing.

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Section: Discussionsupporting

confidence: 92%

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Zhu

Liao

Zhang

et al. 2020

Preprint

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“…The biocompatibility and cytotoxicity of membranes were analyzed in order to investigate the in vitro behavior of the designed materials. From SEM images it was possible to see that the cell's morphology showed a round aspect, indicating good cell interaction with the matrix, maintaining the typical morphology of RAW cells, as previously reported 29,46 . Therefore, these results suggest that the membrane presents a good biocompatibility with cell line tested.…”

Section: Discussionsupporting

confidence: 83%

Terpolymer‐chitosan membranes as biomaterial

Costantino

Belluzo

Oberti

et al. 2021

J Biomedical Materials Res

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The present study shows a novel copolymer synthesis, its application in the membrane design and the physicochemical and biological characterization of the biomaterial obtained. Terpolymer starting diisopropyl fumarate (F), vinyl benzoate (V) and 2‐hydroxyethyl methacrylate (H) was prepared by thermal radical polymerization. This polymer (FVH) was obtained in several monomer ratios and characterized by spectroscopic and chromatographic methods (FTIR, 1H‐NMR and SEC). The best relationship of F:V:H was 5:4:1, which allows efficient interaction with chitosan through cross‐linking with borax to achieve scaffolds for potential biomedical applications. The membranes were obtained by solvent casting and analyzed by scanning electron microscopy (SEM), swelling behavior and mechanical properties. In addition, we studied the possible cytotoxicity and biocompatibility of these materials using a murine macrophage‐like cell line (RAW 264.7) and bone marrow mesenchymal progenitor cells (BMPC), respectively, taking into account their intended applications. The results of this study show that the terpolymer obtained and its combination with a natural polymer is a very interesting strategy to obtain a biomaterial with possible applications in regenerative medicine and this could be extended to other structurally related systems.

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“…Briefly, all hydrogels were cut, weighed (W 0 ) and then incubated in PBS or water for different periods of time. After each time-point, each sample was weighed (W t ) and swelling percentage was evaluated by equation 1 [12,13]. After that, the samples were thoroughly washed with distilled water in order to remove the PBS salts, dried under vacuum and weighed (W dt ) and the degradation percentage as weight loss (W) was evaluated by equation 2.…”

Section: Swelling and Degradation Studiesmentioning

confidence: 99%

“…Cells were rinsed with PBS, fixed with methanol at -20°C for 30 min, washed with distilled water and stained with alcian blue at pH 0.1 overnight. After that time, cells were washed with distilled water and visualized by optical microscopy [13].…”

Section: Cell Cultures and Incubationsmentioning

confidence: 99%

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

Torres

Oberti

Fernández

2021

Journal of Biomaterials Science, Polymer Edition

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Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.

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Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action

Cited by 5 publications

References 39 publications

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Terpolymer‐chitosan membranes as biomaterial

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

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