Influence of environmental factors on degradation of carbendazim by Bacillus pumilus strain NY97-1

Abstract

The influence of environmental factors on the performance of carbendazim degradation by Bacillus pumilus strain NY97-1 was investigated under aerobic conditions. The carbendazim degradation in culture medium was characterised by High-Performance Liquid Chromatography (HPLC) with UV detector. When the initial concentration of carbendazim was 10, 30, 50, 100, 300 mg/L, the degradation rate was 42. 44, 48.97, 77.19, 78.66 and 90.07%, respectively after 24 h at 30°C. Addition of small amount of organic nitrogenous source had a significant influence on carbendazim degradation, while the inorganic nitrogenous source had negative effect. Carbendazim degradation rate varied from 61.55% to 87.76% when pH value within the range of 4-10 after addition of organic nitrogen, the lowest carbendazim degradation percentage of 61.55% was obtained at pH 4.

Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim

“…The results obtained indicated that the degradation rate of carbendazim by the isolate CBW was enhanced with increasing concentrations (1-10 mg l -1 ) and temperature (20-40°C), respectively. Similar to our results, Zhang et al (2009) reported that the degradation rate of carbendazim by Bacillus pumilus NY97-1 was measured to be 42.44-90.07% with its increasing concentrations ranging from 10 to 300 mg l -1 and temperature. However, a different result was found that the degradation rate of carbendazim was not significantly different at different temperatures (15, 25, 30, and 37°C), except that the degradation ability at 15°C decreased slightly (Pattanasupong et al 2004a).…”

“…In our study, neutral condition was favorable for the degradation of carbendazim by CBW, whereas higher or lower pH inhibited the carbendazim degradation. A similar result was reported by Zhang et al (2009), who found that the lowest degradation percentage of carbendazim by Bacillus pumilus NY97-1 was detected at pH 4. Pattanasupong et al (2004b) also found that the degradation ability of the immobilized consortium for carbendazim markedly decreased at pH 4 and 5.…”

“…1-1 (Zhang et al 2005) (Xu et al 2006), Rhodococcus qingshengii sp. djl-6 T (Xu et al 2007), Burkholderia cepacia, Aeromonas hydrophila, Sphingomonas paucimobilis (Kalwasinska et al 2008a, b), and Bacillus pumilus NY97-1 (Zhang et al 2009). In the present study, a new bacterial strain CBW capable of utilizing carbendazim as the sole carbon and energy sources was isolated and identified as Pseudomonas sp.…”

Isolation and Characterization of Carbendazim-degrading Rhodococcus erythropolis djl-11

“…In contrast, previous researches on MBC degradation by Bacillus pumilus NY97-1 [17] and Pseudomonas sp. CBW [5] reported enhanced MBC degradation as concentrations of the fungicide increased.…”

“…CBW were significantly influenced by factors such as pH, temperature and nutrient composition of the culture media [5], [17]. In contrast, MBC degradations by strain djl-11 were not significantly affected by varying the initial pH ranging from 4–9, replacing Mg 2+ with Mn 2+ , Zn 2+ or Fe 2+ or substituting NH 4 NO 3 with organic substrates (peptone, urea, beef and yeast extracts), the latter providing alternative and additional sources of nitrogen and carbon, respectively.…”