Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim

Fang, Hua; Wang, Yiqi; Gao, Chunming; Hu, Yan; Dong, Bi‐Cheng; Yu, Yunlong

doi:10.1007/s10532-010-9353-0

Cited by 68 publications

(39 citation statements)

References 24 publications

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“…However, the complete degradation pathway of 2-AB is not known. For example, Fang et al (2010) studying the degradation of MBC by Pseudomonas sp. CBW revealed that 2-hydroxybenzimidazole (2-HB) was detected as a metabolite of 2-AB.…”

Section: Dynamics Of Tm Disappearance and Amounts Of Mbc And 2-ab In mentioning

confidence: 99%

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

2010

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Degradation of the fungicide thiophanate-methyl (TM) by Enterobacter sp. TDS-1 and Bacillus sp. TDS-2 isolated from sandy soil previously treated with TM was studied in mineral salt medium (MSM) and soil. Both strains were able to grow in MSM supplemented with TM (50 mg l(-1)) as the sole carbon source. Over a 16 days incubation period, 60 and 77% of the initial dose of TM were degraded by strains TDS-1 and TDS-2, respectively, and disappearance of TM was described by first-order kinetics. Medium supplementation with glucose markedly stimulated bacterial growth; while the final rate of TM degradation was reduced by 21 and 27% for strains TDS-1 and TDS-2, respectively as compared to medium with TM only. Moreover, this additional carbon source changed the TM degradation kinetics, which proceeded according to a zero-order model. This effect was linked to substrate competition and/or a strong decrease of medium pH. Isolates degraded TM (100 mg kg(-1)) in soil with rate constants of 0.186 and 0.210 day(-1), following first-order rate kinetics, and the time in which the initial TM concentration was reduced by 50% (DT50) in soils inoculated with strains TDS-1 and TDS-2 were 6.3 and 5.1 days, respectively. Analysis of TM degradation products in soil showed that the tested strains may have the potential to transform carbendazim (MBC) to 2-aminobenzimidazole (2-AB), and may be useful for a bioremediation of MBC-polluted soils.

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Section: Dynamics Of Tm Disappearance and Amounts Of Mbc And 2-ab In mentioning

confidence: 99%

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

2010

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“…As shown in Table 2, the degradation rates of metamitron by the isolate MET at pH 5.0, 7.0, and 9.0 were 0.04, 1.00, and 0.10 mg/l/h, with the corresponding degradation half-lives of 144.4, 1.5, and 52.5 h, respectively, after 9 h of incubation. The results showed that the degradation of metamitron by the isolate MET was significantly ( p ≤ 0.05) faster at pH 7.0 than at pH 5.0 and 9.0, implying that microbial degradation was a pH-dependent process202122. Similarly, Liu et al 23.…”

Section: Resultsmentioning

confidence: 82%

Characterization and genome functional analysis of a novel metamitron-degrading strain Rhodococcus sp. MET via both triazinone and phenyl rings cleavage

Fang

Cao

et al. 2016

Sci Rep

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A novel bacterium capable of utilizing metamitron as the sole source of carbon and energy was isolated from contaminated soil and identified as Rhodococcus sp. MET based on its morphological characteristics, BIOLOG GP2 microplate profile, and 16S rDNA phylogeny. Genome sequencing and functional annotation of the isolate MET showed a 6,340,880 bp genome with a 62.47% GC content and 5,987 protein-coding genes. In total, 5,907 genes were annotated with the COG, GO, KEGG, Pfam, Swiss-Prot, TrEMBL, and nr databases. The degradation rate of metamitron by the isolate MET obviously increased with increasing substrate concentrations from 1 to 10 mg/l and subsequently decreased at 100 mg/l. The optimal pH and temperature for metamitron biodegradation were 7.0 and 20–30 °C, respectively. Based on genome annotation of the metamitron degradation genes and the metabolites detected by HPLC-MS/MS, the following metamitron biodegradation pathways were proposed: 1) Metamitron was transformed into 2-(3-hydrazinyl-2-ethyl)-hydrazono-2-phenylacetic acid by triazinone ring cleavage and further mineralization; 2) Metamitron was converted into 3-methyl-4-amino-6(2-hydroxy-muconic acid)-1,2,4-triazine-5(4H)-one by phenyl ring cleavage and further mineralization. The coexistence of diverse mineralization pathways indicates that our isolate may effectively bioremediate triazinone herbicide-contaminated soils.

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“…However, this bacterial strain could not remove carbendazim effectively from the mineral salts medium supplemented with higher concentration of carbendazim (100 mg L -1 ), i.e. only 9.9 % carbendazim was degraded after 3-day incubation (Fang et al, 2010). In contrast, Pseudomonas sp.…”

Section: Discussion:-mentioning

confidence: 82%

Isolation and Characterization of Carbendazim-Degrading Bacteria in Rice Paddy Soil in Can Tho, Vietnam.

Le¹,

Le²,

Nguyen³

2017

IJAR

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Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim

Cited by 68 publications

References 24 publications

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

Characterization and genome functional analysis of a novel metamitron-degrading strain Rhodococcus sp. MET via both triazinone and phenyl rings cleavage

Isolation and Characterization of Carbendazim-Degrading Bacteria in Rice Paddy Soil in Can Tho, Vietnam.

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