Influence of deuterium depleted water on freeze-dried tissue isotopic composition and morphofunctional body performance in rats of different generations

Джимак, С. С.; Barishev, M. G.; Басов, А. А.; Тимаков, А. А.

doi:10.1134/s0006350914040101

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…Body and internal organs were weighted; blood samples were collected for biochemical assays. Integral index of chronic intoxication (IICHI) were calculated for the liver and kidneys (13).…”

Section: Methodsmentioning

confidence: 99%

“…Isotopic composition of lyophilized organs was determined by a mass spectrometer DELTAplus provided with equipment for sample preparation of isotope hydrogen analysis H/Device (Finnigan, Germany) (13).…”

Section: Methodsmentioning

confidence: 99%

“…Deuterium-depleted water (-743%) was obtained on the plant created by the Kuban State University (13). Mineralization of DDW was conducted by addition of mineral salts (mineralization 314 -382 mg/L: hydro carbonates 144 -180 mg/L, sulfates < 1 mg/L, chlorides 60 -76 mg/L, calcium 6 mg/L, magnesium 3 mg/l, sodium 50 -58 mg/L and potassium 50 -58 mg/L).…”

Section: Methodsmentioning

confidence: 99%

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Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Сергеевич

Басов

Елкина

et al. 2018

Jundishapur J Nat Pharm Prod

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The objective of the current study was to evaluate the influence of deuterium-depleted water (DDW) consumption on healthy animals and animals with hepatorenal toxicity. The influence of deuterium-depleted water on hepatorenal toxicity was investigated in Wistar rats. Deuterium-depleted water consumption during 42 days caused changes in isotopic D/H composition of rats' plasma and lyophilized tissues of internal organs (liver and kidney). Moreover, deuterium-depleted water influenced plasma biochemical parameters. Concentration of creatinine and bilirubin, as well as aspartate aminotransferase and alanine aminotransferase activities were reduced in plasma of rats with hepatorenal toxicity. Deuterium-depleted water consumption during 42 days influenced the body and internal organ weights while integral index of chronic intoxication did not increase in rats with chronic intoxication. It was also revealed that deuterium-depleted water consumption did not display any toxic effects.

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“…Body and internal organs were weighted; blood samples were collected for biochemical assays. Integral index of chronic intoxication (IICHI) were calculated for the liver and kidneys (13).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Сергеевич

Басов

Елкина

et al. 2018

Jundishapur J Nat Pharm Prod

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“…For immunocompetent polypeptides extraction it was used water with reduced deuterium concentration -704.6 ‰, which was produced by electrolysis with drying of obtained electrolysis gas, electrolysis gases conversion in water and water vapor condensation [23]. Deuterium concentration control in obtained water was determined on pulsed NMR spectrometer JEOL JNM-ECA 400MHz in "Kuban State University" [24,25].…”

Section: Extractionmentioning

confidence: 99%

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

Fedulova

Vasilevskaya

Kotenkova³

et al. 2017

J. Pharm. Nutr. Sci.

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The influence of protein-peptide compounds, extracted with deuterium water as solubilizer agent from mixture of Sus scofa thymus, spleen and lymph nodes extracts, was carried out on Wistar rats with cytostatic-induced immunodeficiency model. Intragastric administration of extract fraction with molecular weight more than 30 kDa, did not lead to significant changes. Thus, polypeptide compounds with molecular weight less than 30 kD, extracted from Sus scofa immune organs, significantly increased T-lymphocytes amount, affected macrophages system by T-helper (CD4) stimulation and activated cycle of complementary cascade. Differences between the experimental results in T-helper synthesis activation (CD4) in animals treated with the medium and low molecular weight fractions can be explained by the ability of biomolecules having a molecular weight less than 30 kDa (in particular, isolated from the spleen, which, in its cellular structure contains key regulatory factors) to change the speed of cell differentiation.

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“…These all leads to oxidation stress as there is discordance of prooxidant and antioxidant cell resources. That is why in cases where metabolic defects are the result of a severe intoxication and oxidation metabolism failure, it is possible to use antioxidative medications in a complex therapy [24,25], including products with a modified isotopic composition [26,27].…”

Section: Introductionmentioning

confidence: 99%

Changes in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the water

Быков

Chikhladze

Щава

et al. 2018

Vestn. Ross. univ. družby nar., Ser. Med.

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Abstract. The aim of the research was to study the effect of the water menu with a low D/H on morphological hepatocytes parameters in rabbits in modeling of an acute hypoxic liver damage. Methods: morphological research of liver tissues of the experimental animals had been done by cytological and histological methods, which had been prepared impression smear of the studying organ after which it had been stained with azur-eosin according to a common procedure; the blood plasma had been done by the pulsing nuclear magnetic resonance spectrometer JEOL JNM-ECA 400MHz, evaluate an isotopic composition of rat lyophilized liver tissues had been used an isotopic mass-spectrometer DELTA plus . Results:There are the statistics presented in the given study reveal the feature of the water menu with the low deuterium concentration decreases the liver tissue damage in hypoxia simulating by the compression of a hepatoduodenal ligament. It was demonstrated that the use of the deuterium depleted water (DDW) reliably decreases the deuterium concentration in a blood plasma (by 32%) and in liver tissue (by 9%), which shows the effectiveness of the correlation of isotopic composition of internal environment and organs by the products with the low deuterium concentration.Conclusion: It has been established that the use of the DDW as a hepatoprotective measure is equal in its effectiveness to some of lipophilic antioxidants. This allows to use vegetative antioxidant factors and a water menu with a low deuterium concentration (40 ppm) as a combined measure, in order to correct morphological liver defects in its ischemic damage.

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Influence of deuterium depleted water on freeze-dried tissue isotopic composition and morphofunctional body performance in rats of different generations

Cited by 17 publications

References 13 publications

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity

Changes in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the water

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