Influence of Culture Conditions on Growth and Survival of Conidia of Trichoderma spp. Coated on Seeds

Cliquet, Sophie; Scheffer, R. J.

doi:10.1080/09583159730875

Cited by 9 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seeds were coated with slurry at 23% w/w and dried in the laminar flow at 20°C for 12 h (Watt and Walker ). Formulation 4: 2% methylcellulose slurry was prepared with T. atroviride spore suspension (10 8 spores ml −1 ). The slurry was coated on seeds at 16·6% w/w, and then dried in laminar flow for 12 h at 20°C (Cliquet and Scheffer ). Formulation 5: A mixture of 4% w/w xanthan gum, 4% w/w canola oil were mixed with 92% w/w T. atroviride aqueous spore suspension (10 10 spores ml −1 ) to form a gel. Seeds were coated with gel at 8% w/w followed by addition of clays (bentonite 4% and hydrated magnesium silicate 12%).…”

Section: Methodsmentioning

confidence: 99%

“…Formulation 4: 2% methylcellulose slurry was prepared with T. atroviride spore suspension (10 8 spores ml À1 ). The slurry was coated on seeds at 16Á6% w/w, and then dried in laminar flow for 12 h at 20°C (Cliquet and Scheffer 1997). Formulation 5: A mixture of 4% w/w xanthan gum, 4% w/w canola oil were mixed with 92% w/w T. atroviride aqueous spore suspension (10 10 spores ml À1 ) to form a gel.…”

Section: Seed-coating Formulationsmentioning

confidence: 99%

See 1 more Smart Citation

Formulations for delivering Trichoderma atroviridae spores as seed coatings, effects of temperature and relative humidity on storage stability

Swaminathan

Koten

et al. 2016

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Seed-coating Formulationsmentioning

confidence: 99%

Formulations for delivering Trichoderma atroviridae spores as seed coatings, effects of temperature and relative humidity on storage stability

Swaminathan

Koten

et al. 2016

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Solid state fermentation (SSF) offers numerous advantages over liquid fermentation (Pandey, 1991(Pandey, , 1992(Pandey, , 1994 and could be an attractive alternative for this. SSF has been considered a useful system for spores production (Cliquet & Scheffer, 1997;Muñoz et al, 1995;Roussos, 1987). However, little is known about the kinetics of SSF processes that hinders further development and scale-up of particular processes and their consequent technologies.…”

Section: Introductionmentioning

confidence: 99%

Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol

Rodríguez-León

Domenech

León

et al. 1999

Braz. arch. biol. technol.

View full text Add to dashboard Cite

Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR) pattern, which corresponded to the order of 10(9)spores/g moist material. The specific growth rate, maintenance coefficient and the yield based on O2 consumption were 0.108 h-1, 0.001 g.O2/g.biomass.h and 2.7 g biomass/g O2 consumed, respectively.
Esporos de Tricoderma harzianum Nº 53 foram produzidos por fermentação no estado sólido (FES) utilizando bagaço de cana como suporte e melaço de cana como fonte de carbono. Diferentes fontes de nitrogênio foram testadas (uréia, (NH4)2 SO4 , NH4H2PO4 e (NH4)2HPO4 ) na produção de esporos. As mais elevadas concentrações de esporos (10(9) esporos/g de suporte úmido) foram obtidas utilizando a uréia como fonte de nitrogênio. O efeito da temperatura e umidade inicial foram estudadas através da utilização da planificação experimental utilizando um modelo 2². Não foi encontrada diferença estatística na produção de esporos na faixa de temperatura compreendida entre 30-35 ° C e umidade inicial de 60-70%. Os parâmetros biotecnológicos foram determinados através da taxa de oxigênio consumido (OUR) correspondente a uma produção de 10(9) esporos/g de suporte úmido. A taxa de crescimento especifico, coeficiente de manutenção e rendimento foram calculados em função do O2 consumido, cujos valores foram 0.108 h-1 , 0.001 g O2/g biomassa/h e 2.7 g biomassa/g O2 consumido respectivamente

show abstract

“…A prodigious amount of research has been conducted on alteration of seed germination characteristics. Typically, the underlying objectives of seed treatments are focused on alleviating stress associated with the soil environment and/or on directly increasing plant growth (Taylor and Harman, 1990) The most common seed treatments alleviate biotic stress by reducing the damage caused by seed or soil borne pests and pathogens (e.g., insects and fungi) on seeds and seedlings (Silininger et al, 1996; Cliquet and Scheffer, 1997; Shah‐Smith and Burns, 1997). Alleviation of abiotic stress is another objective in seed treatment research, and has been achieved by modifying the soil water‐oxygen relations around a germinating seed (Leaver and Roberts, 1984; Huang and Hsiao, 1987).…”

mentioning

confidence: 99%

Modification of Seed Germination Performance through Cold Plasma Chemistry Technology

et al. 2000

View full text Add to dashboard Cite

This study was conducted to determine if an alternate seed treatment approach based on plasma chemistry would offer a more viable alternative over traditional seed coating technologies. Seed germination characteristics were modified in five agricultural species by coating the surface of the seeds with macromolecules from a cold plasma process using a rotating plasma reactor. The source gas entering the plasma chamber during the reaction process determined the type of coating, and coatings were typically much less than 5.0 μm in thickness. To delay germination we utilized two different hydrophobic source gases, carbon tetrafluoride (CF4) or octadecafluorodecalin (ODFD). Seeds of radish (Raphanus sativus) and two pea cultivars (Pisum sativum cv. Little Marvel, P. sativum cv. Alaska) treated with CF4, resulted in a significant delay in germination compared with untreated controls. Similarly, plasma treatment with ODFD significantly delayed germination in soybean [Glycine max (L.) Merr.], corn (Zea mays L.), and bean (Phaseolus vulgaris L.) seeds. The degree of delay was dependent on the amount of coating applied, with an increased thickness of coating resulting in a greater delay in germination. To enhance germination we treated seeds with cyclohexane, or with a gas such as aniline or hydrazine. Seeds treated with cyclohexane resulted in a significant acceleration in germination percentage for soybean but not corn seeds, while hydrazine‐treated corn seed showed a small acceleration over control seed. However, both aniline‐treated soybean and corn seed had a significant acceleration in germination percentage. Tests of water uptake determined that the major mode of action of the plasma coatings was largely on the rate of imbibition. These results demonstrate a potentially important technique to modify seed germination characteristics in agricultural plant species.

show abstract

Influence of Culture Conditions on Growth and Survival of Conidia of Trichoderma spp. Coated on Seeds

Cited by 9 publications

References 15 publications

Formulations for delivering Trichoderma atroviridae spores as seed coatings, effects of temperature and relative humidity on storage stability

Formulations for delivering Trichoderma atroviridae spores as seed coatings, effects of temperature and relative humidity on storage stability

Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol

Modification of Seed Germination Performance through Cold Plasma Chemistry Technology

Contact Info

Product

Resources

About