Formulations for delivering <i>Trichoderma atroviridae</i>
 spores as seed coatings, effects of temperature and relative humidity on storage stability

Swaminathan, J.; Koten, Chikako van; Hv, Henderson; Jackson, Trevor A.; Wilson, Michael J.

doi:10.1111/jam.13006

Cited by 23 publications

(15 citation statements)

References 21 publications

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“…It is well-known that chitosan application on seeds is beneficial in order to enhance the germination rate and/or yield in many agricultural crops, such as maize [54], wheat [6], soybean [55], canola [10], artichoke [35], or ajowan [56]. However, the application of chitosan and propolis did not enhance the germination rates in the present study.…”

Section: Discussioncontrasting

confidence: 82%

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Application of Bioactive Coatings Based on Chitosan and Propolis for Pinus spp. Protection against Fusarium circinatum

Silva-Castro

Martín-Ramos

Pinto

et al. 2018

Forests

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Pine pitch canker (PPC) is a major threat to pine forests worldwide because of the extensive tree deaths, reduced growth, and degradation of timber quality caused by it. Furthermore, the aggressive fungus responsible for this disease (Fusarium circinatum) can also infect pine seeds, causing damping-off in young seedlings. This study proposes an approach based on coating treatments consisting of natural products to ensure seed protection. Seeds from two pine species (the most sensitive to this disease, Pinus radiata D. Don, and a more resistant one, Pinus sylvestris L.) were coated with single and binary mixtures of low and medium molecular weight chitosan and/or ethanolic-propolis extract. The germination rate, pre- and post-emergence mortality, total phenolic content, and radical scavenging activity were assessed. All treatments, and especially the one based on chitosan oligomers, had a beneficial impact on P. sylvestris seedlings, significantly enhancing survival rates and displaying a positive influence on the total phenolic content and on the seedlings’ radical scavenging activity. Conversely, non-significant negative effects on germination percentages were observed in the case of P. radiata seeds. The proposed treatments show promise for the protection of P. sylvestris seedlings against PPC.

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Section: Discussioncontrasting

confidence: 82%

“…Consequently, natural substances and biological agents-such as starch [9] and Trichoderma spp. [10,11], respectively-are receiving increasing attention as environmentally friendly alternatives [12,13].…”

Section: Introductionmentioning

confidence: 99%

Application of Bioactive Coatings Based on Chitosan and Propolis for Pinus spp. Protection against Fusarium circinatum

Silva-Castro

Martín-Ramos

Pinto

et al. 2018

Forests

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“…These trials also indicated that the biological coating was applicable with conventional machinery and compatible with pesticides use (herbicides and double coating with crow repellent), suggesting an IDM strategy. Regarding storage temperature, cold storage at 4°C is considerable in Europe (Swaminathan et al 2016). Keeping coated seeds in the fridge takes up space compared to small package boxes.…”

Section: Discussionmentioning

confidence: 99%

Trichoderma atroviride as a promising biocontrol agent in seed coating for reducing Fusarium damping‐off on maize

et al. 2020

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Aims: The objective of this work was to identify a fungal strain showing potential biocontrol abilities against two Fusarium damping-off agents and to test it as a Biological Control Agent (BCA) in maize seed coating under field conditions. Methods and Results: A collection of native fungal strains associated with maize in Belgium was screened for antagonistic potential against Fusarium avenaceum and Fusarium culmorum. The strain with highest biocontrol potential was identified as an endophytic Trichoderma atroviride BC0584. In greenhouse, it significantly improves the emergence of seedlings infected by F. avenaceum or F. culmorum pathogens. In most field trials carried out during the season 2017, it significantly increased the emergence rate of infected seedlings compared to untreated seeds. One slurriable powder formulation allows BCA conidia to survive over a 6-month storage period at 4°C. Conclusions: The fungal BC0584 strain is a promising BCA that could be an alternative to synthetic fungicides. It is adapted to local environmental conditions, is easily and cheaply produced and can be stored in a low-cost formulation. Significance and Impact of the Study: In Belgium, this is the first study to use a T. atroviride native strain against Fusarium damping-off on maize crop. Modes of action and required conditions for ensuring high biocontrol activity in the field have still to be investigated.

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“…A recent study has highlighted the complex interactions between formulation methods/excipients and environmental conditions (relative humidity and temperature) on stability of Trichoderma spores applied to maize seed (Swaminathan et al 2016 ). Spore survival over 6 months varied significantly between five formulations held under different environmental conditions, suggesting that there is not one formulation suitable for storage under all conditions and that changes in relative humidity affect spore survival in some formulations more than others.…”

Section: Challenges In Development Of Microbial Inoculant Seed Treatmmentioning

confidence: 99%

Microbial inoculation of seed for improved crop performance: issues and opportunities

O’Callaghan

2016

Appl Microbiol Biotechnol

343

216

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There is increasing interest in the use of beneficial microorganisms as alternatives to chemical pesticides and synthetic fertilisers in agricultural production. Application of beneficial microorganisms to seeds is an efficient mechanism for placement of microbial inocula into soil where they will be well positioned to colonise seedling roots and protect against soil-borne diseases and pests. However, despite the long history of inoculation of legume seeds with Rhizobia spp. and clear laboratory demonstration of the ability of a wide range of other beneficial microorganisms to improve crop performance, there are still very few commercially available microbial seed inoculants. Seed inoculation techniques used for research purposes are often not feasible at a commercial scale and there are significant technical challenges in maintaining viable microbial inocula on seed throughout commercial seed treatment processes and storage. Further research is needed before the benefits of a wide range of environmentally sensitive potential seed inoculants can be captured for use in agriculture, ecosystem restoration and bioremediation. There is no single solution to the challenge of improving the ability of seed inoculants to establish and function consistently in the field. Development of novel formulations that maintain the viability of both inoculant and seed during storage will result from multidisciplinary research in microbial and seed physiology and adjuvant chemistry.

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Formulations for delivering Trichoderma atroviridae spores as seed coatings, effects of temperature and relative humidity on storage stability

Abstract: Most published formulation papers/patents only report survival of organisms over time at a single or limited number of temperatures and RH. For the first time, this study shows how different formulations are better suited to certain temperature and RH combinations.

Cited by 23 publications

References 21 publications

Application of Bioactive Coatings Based on Chitosan and Propolis for Pinus spp. Protection against Fusarium circinatum

Application of Bioactive Coatings Based on Chitosan and Propolis for Pinus spp. Protection against Fusarium circinatum

Trichoderma atroviride as a promising biocontrol agent in seed coating for reducing Fusarium damping‐off on maize

Microbial inoculation of seed for improved crop performance: issues and opportunities

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