Production of spores of Trichoderma harzianum on sugar cane molasses and bagasse pith in solid state fermentation for biocontrol

Abstract

Solid state fermentation was carried out for the production of spores from Trichoderma harzianum No 53 using sugar cane bagasse pith as solid matrix and sugar cane molasses as carbon and energy source. Different nitrogen sources such as urea, (NH4)2SO4 , NH4H2PO4 and (NH4)2HPO4 were added in the media to test their effect on spores production. Among these, urea was found most useful that resulted high no of spores (1x10(9)/gDM). The influence of temperature and initial moisture of the substrate was studied through a 2² experimental plan design. No statistical differences were found within the range of 30-35ºC and 60-70% for temperature and moisture respectively. The biotechnological parameters of the process were derived from the Oxygen Uptake Rate (OUR) pattern, which corresponded to the order of 10(9)spores/g moist material. The specific growth rate, maintenance coefficient and the yield based on O2 consumption were 0.108 h-1, 0.001 g.O2/g.biomass.h and 2.7 g biomass/g O2 consumed, respectively.<br>Esporos de Tricoderma harzianum Nº 53 foram produzidos por fermentação no estado sólido (FES) utilizando bagaço de cana como suporte e melaço de cana como fonte de carbono. Diferentes fontes de nitrogênio foram testadas (uréia, (NH4)2 SO4 , NH4H2PO4 e (NH4)2HPO4 ) na produção de esporos. As mais elevadas concentrações de esporos (10(9) esporos/g de suporte úmido) foram obtidas utilizando a uréia como fonte de nitrogênio. O efeito da temperatura e umidade inicial foram estudadas através da utilização da planificação experimental utilizando um modelo 2². Não foi encontrada diferença estatística na produção de esporos na faixa de temperatura compreendida entre 30-35 ° C e umidade inicial de 60-70%. Os parâmetros biotecnológicos foram determinados através da taxa de oxigênio consumido (OUR) correspondente a uma produção de 10(9) esporos/g de suporte úmido. A taxa de crescimento especifico, coeficiente de manutenção e rendimento foram calculados em função do O2 consumido, cujos valores foram 0.108 h-1 , 0.001 g O2/g biomassa/h e 2.7 g biomassa/g O2 consumido respectivamente

Improved calibration of a solid substrate fermentation model

“…However, the oxygen yields are similar to (even smaller than) those previously reported in SSF-between 3.8 and 2.7 g/g- (Soccol et al 1993;Rodríguez-León et al 1999;Brand et al 2001;Brand et al 2002). Furthermore, a recent publication by Chavez-Parga et al (2008) reported yields similar (Yx/s = 0.22-1.87 g/g and Yx/n = 11-49.4 g/g) to the ones reported by Gelmi et al (2002).…”

In Vitro Effect of Molasses Concentration, pH, and Time on Chromium Removal by Trichoderma spp. from the Effluents of a Peruvian Tannery

“…Molasses, used at two concentrations (0.5% and 1%), was used as a substrate for the fungus, as it can be used as a source of carbon and nitrogen for the growth of Trichoderma [38]. Treatment 3 produced lower total chromium values at both eight and twelve days of evaluation.…”

Isolation of Trichoderma Spp. from Desert Soil, Biocontrol Potential Evaluation and Liquid Culture Production of Conidia Using Agricultural Fertilizers

“…Evaluation of different concentrations of vinaze in the culture media indicates that adding 10 ml.L -1 increased conidial concentration. Formulations containing carbon sources with non-defined composition has shown good results on T. harzianum, reaching concentrations up to 1 × 10 9 spore.g -1 with molasses adjusted to 37% of total sugars on media [42]; also, media using molasses from sugarcane are used to induce T. asperellum to produce extracellular lignocellulosic enzymes [43]. Regarding the nitrogen source, the highest biomass was induced with KNO 3 •K 2 SO 4 , whereas the lowest values were obtained with (NH 4 all the experiments presented in this study, pH was little affected over time and probably did not influence conidia or biomass production, as stated from Lewis and Papavizas for T. harzianum, where conidial production was not influenced by the initial pH of the media or their continuous maintenance at pH 4 or 7 [45].…”