Influence of Coenzyme Q₁₀ on release of pro‐inflammatory chemokines in the human monocytic cell line THP‐1

Abstract

Coenzyme Q_{10} (CoQ_{10}) is an obligatory element in the mitochondrial electron transport system and functions as a potent antioxidant of lipid membranes. In-vivo and in-vitro studies indicate an involvement of CoQ_{10} in inflammatory pathways. Here we studied in the human monocytic cell-line THP-1 the influence of CoQ_{10} on LPS-induced secretion of the pro-inflammatory chemokines Macrophage inflammatory protein-1 alpha (MIP-1alpha), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemoattractant protein-1 (MCP-1). In comparison to unstimulated cells, LPS leads to 22-, 3- and 4.5-fold higher levels of MIP-1alpha, RANTES and MCP-1 in the cell culture medium, respectively. Pre-incubation of cells with 10 microM CoQ_{10} resulted in a significant decrease of LPS-induced MIP-1alpha and RANTES secretion to 55.04% (p = 0.02) and 76.84% (p = 0.04), respectively. In conclusion, CoQ_{10} reduces the LPS-induced secretion levels of the pro-inflammatory chemokines MIP-1alpha and RANTES in the human monocytic cell line THP-1. These data suggest that CoQ_{10} possesses anti-inflammatory properties.

Ubiquinol reduces gamma glutamyltransferase as a marker of oxidative stress in humans

“…Furthermore, CoQ 10 is necessary for pyrimidine biosynthesis while also being a cofactor for uncoupling proteins [3]. It has also been identified as a modulator of gene expression [4-6], inflammatory processes [7-9] and apoptosis [10,11]. The reduced form of CoQ 10 , ubiquinol (Q 10 H 2 ), serves as a potent antioxidant in mitochondria and lipid membranes as well as a regenerator of other lipid soluble antioxidants [12].…”

Coenzyme Q10 supplementation lowers hepatic oxidative stress and inflammation associated with diet-induced obesity in mice

“…CoQ10 is also involved in oxidative phosphorylation as an electron transporter and is therefore involved in energy homeostasis [17][18][19][20]. Data obtained in vitro even suggest its antiinflammatory potency [21][22][23][24][25]. However, the effect of CoQ10 given orally remains only poorly explored in the context of obesity and diabetes.…”

Protection Against Oxidative Stress and “IGF-I Deficiency Conditions”

“…CoQ10 is a strong anti-oxidant that confers resistance to mitochondrial damage by decreasing ROS/RNS production and that may suppress the production of proinflammatory substances, like nuclear factor κ B (NFκB) gene expression and the production of pro-inflammatory cytokines [95][96][97][98][99][100]. CoQ10 and vitamin E are lipophylic antioxidants that are target to mitochondrial matrix by Tripenylphoshonium ion (TPP+) (mitoQ and mitovitamin E).…”