Influence of catecholamines on biofilm formation by Salmonella Enteritidis

Hiller, Caroline Carniel; Lucca, Vivian; Carvalho, Daiane; Borsoi, Anderlise; Borges, Karen Apellanis; Furian, Thales Quedi; Nascimento, Vladimir Pinheiro do

doi:10.1016/j.micpath.2019.02.032

Cited by 20 publications

(15 citation statements)

References 47 publications

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“…Their functional association with the microbiota-gut-brain axis seems to be a functional loop. Some enteric bacteria, such as members of the genera Klebsiella, Pseudomonas, Enterobacter, and Staphylococcus, respond to catecholamine molecules by intensified proliferation and/or increased motility, biofilm formation, and virulence (69)(70)(71). Some, as for example Escherichia coli, Proteus vulgaris, Serratia marcescens, and Bacillus subtilis, produce molecules with neuroactive potential (72).…”

Section: Dopamine Epinephrine and Norepinephrinementioning

confidence: 99%

Microbiota-Gut-Brain Signaling: A Minireview

Kubelková¹,

Macela²

2020

Mil. Med. Sci. Lett.

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The gut microbiota of vertebrates, including humans, constitutes an integral genomic part that, together with the genome of the host, may be included under the umbrella concept of hologenome, which itself can be seen as one of the possible tools for evolution. Present-day lifestyles, technologically processed nutrients, and various diseases impact significantly upon composition of the intestinal microbiota. Knowledge recently brought to light has shown the gut microbiota to be a component of the microbiota-gut-brain axis having feedback effects on physiological and psychological processes of the host organism and its health. This minireview summarizes current knowledge and opinions on the importance of the microbiota-gut-brain axis and discusses possibilities for beneficially modulating one of the organism's most vital axes.

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Section: Dopamine Epinephrine and Norepinephrinementioning

confidence: 99%

Microbiota-Gut-Brain Signaling: A Minireview

Kubelková¹,

Macela²

2020

Mil. Med. Sci. Lett.

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“…Several studies report the association of catechol with Salmonella virulence. Salmonella have the ability to produce auto-inducers 3 (AI-3), which have a similar chemical structure as the catecholamine from the catechol family [64,65]. In chicken and in pigs, studies have already showed that a treatment with catecholamine, e.g.…”

Section: Catechol Degradation Pathwaymentioning

confidence: 99%

“…A study in mice on Salmonella Typhimurium showed that molecules from the catechol family increase the iron availability for Salmonella by chelating iron III in their aromatic function and increasing iron accumulation in macrophages, which in turn facilitate Salmonella colonisation [68]. Besides, catechol plays a role of quorum sensing signal for the production of bio lm, thus increasing the virulence of Salmonella during host infection [64,69]. Outside the host, this bio lm increases the Salmonella capacity to resist on eggs or meat, rising salmonellosis risks in human [70].…”

Section: Catechol Degradation Pathwaymentioning

confidence: 99%

Impact of Host Genetics on Caecal Microbiota Composition and on Salmonella Carriage in Chicken

Cazals¹,

Estellé²,

Bruneau³

et al. 2020

Preprint

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Background Salmonella Enteritidis (SE) is one of the major causes of human foodborne intoxication through the consumption of contaminated poultry products. Genetic selection of animals more resistant to Salmonella carriage and the modulation of gut microbiota are two promising ways of decreasing individual Salmonella carriage. This study aims to identify the main genetic and microbial factors controlling the individual levels of Salmonella carriage in chickens (Gallus gallus) in controlled experimental conditions. Two-hundred and forty animals from the White Leghorn inbred lines, N and 61, were infected by SE at 7 days of age. After infection, animals were kept in isolators to reduce the recontamination of birds by Salmonella. Caecal contents were sampled at 12 days post-infection and used for DNA extraction. Microbiota DNA was used to measure individual counts of SE by digital PCR and to determine the bacterial taxonomic composition through a 16S rRNA gene high-throughput sequencing approach. Results Results confirmed that the N line is more resistant to Salmonella carriage than the 61 line, and that intra-line variability is higher for the 61 line. Furthermore, the 16S analysis showed strong significant differences in microbiota taxonomic composition between the two lines. Out of 617 Operational Taxonomic Units (OTUs), over 390 were differentially abundant between the two lines. Furthermore, within the 61 line, we found a difference in the microbiota taxonomic composition between high and low Salmonella carriers, with 39 differentially abundant OTUs. Finally, via metagenome functional prediction based on 16S data, we identified several metabolic pathways potentially associated to microbiota taxonomic differences (e.g. butyrate metabolism) between high and low carriers. Conclusions Overall, this study demonstrates that the caecal microbiota composition of the N and 61 lines is influenced by the host genetics, which could be one of the reasons why these lines differ for their Salmonella carriage in experimental infection conditions.

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“…Infections caused by Salmonella are an ongoing worldwide public health problem, often found as a source of nosocomial infections, which cause significant socio-economic burdens (Kuhns et al, 2012;Hiller et al, 2019). In terms of distribution, Salmonella are extensively represented within the environment as major zoonotic food-borne pathogens, causing outbreaks (Dieckmann & Malorny, 2011).…”

Section: Introductionmentioning

confidence: 99%

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

Novák

Kochlanova

Tvrda

et al. 2019

CBUP

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Introduction: Infections caused by Salmonella are an ongoing worldwide public health problem, often found as a source of nosocomial infections, which cause significant socio-economic burdens. Salmonella is a major food-borne pathogen causing primarily gastrointestinal diseases as well as other localized and systemic infections and extraintestinal complications. MALDI-TOF MS is a new method used by clinical laboratories for rapid, reliable, cost-effective and user-friendly diagnosis of many medical important bacteria of public health interest. The use of this technique improves early identification of genus Salmonella on the species, subspecies and even serovar level, which has a positive impact on public health. Objectives: The aim of the study was to evaluate the importance of MALDI-TOF mass spectrometry for rapid identification of epidemiologically important Salmonella serovars. Based on the latest knowledge about specific biomarker molecules the possibility to identify Salmonella enterica subsp. enterica serovar Enteritidis was verified, which is one of the most common serovars present in Europe associated with gastrointestinal diseases. For serovar Enteritidis a unique mass peak at m/z 6,036 was used. Methods: 140 clinical Salmonella isolates were collected from January to October 2017. Serotyping of Salmonella species, subspecies and serovars was performed by slide agglutination technique: 139 isolates were identified as Salmonella enterica subsp. enterica and one isolate as Salmonella enterica subsp. diarizonae (IIIb). From 139 isolates of Salmonella enterica subsp. enterica the following serovars were detected: 108 Enteritidis, 12 Typhimurium, 6 Infantis, 3 Agona, 3 Derby, 7 others. All isolates were identified also by MALDI-TOF MS as Salmonella spp. For all isolates a unique mass peak at m/z 6.036 was used, which is considered to be relevant for serovar Enteritidis according to the most recent known data. Results: 103 isolates from a total of 108 slide agglutination positive isolates for serovar Enteritidis showed a specific mass signal at m/z 6,036 (+/-). 5 isolates did not contain this specific protein. After repeated analysis from re-culture, the specific protein was found also in the remaining 5 isolates. 32 serovars other than Enteritidis did not contain this specific biomarker molecule. Conclusion: We can confirm that MALDI-TOF MS is a rapid and reliable method to identify of the most common serovar Salmonella Enteritidis based on the diagnostic marker peak at m/z 6,036 identified by recent studies. This unique mass signal showed 100% specificity and 95% sensitivity for Enteritidis serovar in our study. We can conclude that the determination of this frequently present serovar is significantly accelerated by MALDI-TOF MS. The rapid and reliable diagnosis is important for the early treatment and prevention of the spread of salmonellosis with a positive impact on public health.

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Influence of catecholamines on biofilm formation by Salmonella Enteritidis

Cited by 20 publications

References 47 publications

Microbiota-Gut-Brain Signaling: A Minireview

Microbiota-Gut-Brain Signaling: A Minireview

Impact of Host Genetics on Caecal Microbiota Composition and on Salmonella Carriage in Chicken

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

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