Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes

Freitas, Mariana Vaini de; Netto, Rita de Cássia Mascarenhas; Huss, Juliana Carla da Costa; Souza, Tatiana Maria T. de; Costa, Júnia de Oliveira; Firmino, Cynthia Barbosa; Penha-Silva, Nilson

doi:10.1016/j.tiv.2007.07.010

Cited by 65 publications

(42 citation statements)

References 16 publications

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“…The substance responsible for the anti-haemolysin activity of tea may be catechins, because studies have shown that catechins purified from green tea also inhibited the haemolytic activities of Staphylococcus α-toxin and Vibrio parahaemolyticus' toxin (Sachie et al, 1989). Other studies suggested that flavonoids could be incorporated in erythrocyte membranes and improve their stability or that the exacerbation of the van der Waals contacts inside the lipid bilayer to flavonoids could be a source of membrane stabilization (de Freitas et al, 2008;Mhamed et al, 2015). This shows that extracts of tea can be used in treating some haemolytic diseases.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antihaemolytic Activities of Crude Extracts of Some Commonly Used Tea and Coffee in Nigeria

Olajubu

2017

jtsr

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Current indiscriminate abuse of existing antibiotics in clinical and veterinary treatments lead to an upsurge in antimicrobial resistant strains of microorganisms and aggressive search for alternatives which are readily available, less expensive with little or no side effect. Tea and Coffee are beverages consumed daily in every household in Nigeria. This study examines the antimicrobial and anti-haemolytic properties of commonly available Tea and Coffee in Nigerian market. The antimicrobial potencies of the extracts were assessed through disc diffusion method on pathogens of both man and animal origin while the anti-haemolytic assay was carried out through colorimetric method. The extracts were slightly acidic at full strength and no antifungal property was observed. Broad spectrum and bactericidal effects were observed against Staphylococcus aureus, Salmonella pullorum, Shigella dysenteriae and Streptococcus pneumonia. These activities were concentration dependent. Very poor activity was observed against Escherichia coli. Bactericidal rate of coffee was at 6hrs but ranged between 18 and 24 hrs for tea extracts. Tea extracts greatly inhibited the haemolytic potential of alpha toxins while coffee performed poorly. Tea and Coffee could thus serve as supportive treatment for some bacterial infections without fear of side effects, since they are naturally taken as daily beverages.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antihaemolytic Activities of Crude Extracts of Some Commonly Used Tea and Coffee in Nigeria

Olajubu

2017

jtsr

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“…These preliminary results suggest that poly-pPD contributes in preventing the penetration of water molecules within the RBC in hypotonic liquid, thus protecting them from hemolysis. The formation of poly-pPD solution by chemical oxidation has been described in the literature [8][9][10][11][12].…”

Section: Discussionmentioning

confidence: 99%

“…The stability of the membrane represents the ability of the biological complex to maintain its structure under various conditions such as heat, hypotonia, extreme pH, presence of solutes and oxidative stress. This may be caused by a number of molecules including p-phenylenediamine (pPD) or p-aminobenzene [8][9][10][11][12]. This is an aromatic amine of mineral origins of the family of aniline, used since 1863 by women as black hair dye for coloring hair, or adjuvant henna for tattoos in several countries in Africa and the Middle East.…”

Section: Introductionmentioning

confidence: 99%

Chemical and Biological Analyses of the Role of the Poly- Para- Phenylenediamine in the Erythrocytes Resistance in Hypotonic Environment

Srhayri¹,

Naimi²,

Takky³

et al. 2016

J Membra Sci Technol

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potential scan rate of 200 mVs-1, two voltametric waves on the platen; which corresponds to two successive and reversible processes to a single electron, leading to the formation of a diamine [24].Moreover, the genotoxicity studies in vitro have shown that pPD, like several other aromatic amines which belong to the aniline family can react with DNA and cause breaks . Picardo et al. have shown that pPD at high concentrations and for long exposure time induced cell cycle block and toxicity, but at low concentrations (10 µg/mL)and for shorter periods exposure (30 min) increased keratinocyte proliferation [39]. Habti et al. [6] have shown that pPD at low concentrations (5µg/mL) and for 2h exposure time gives RBC temporary resistance to hemolysis in hypotonic blend, the mechanism remains unexplained till now [6]. The aim of our study is to contribute to elucidate the molecular mechanism responsible for this temporary resistance. Material and Methods Collection of human blood sampleBlood samples from volunteer donors, and healthy aged 25-35 years were collected in tubes of 7 mL containing EDTA. Blood donors were nonsmokers and had no history of allergic reaction to pPD or AbstractThe high chemical and biological reactivity of para-phenylenediamine (pPD) has been illustrated in several studies, including polymerization genotoxicity and oxidative stress at the level of the plasma membrane cells. However, its reactivity with red blood cells (RBC) was rarely explored.The aim of this study is to help expound the molecular actions of para-phenylenediamine in hypotonic environment of human red blood cells by conducting biological tests for the globular resistance and physicochemical cyclic voltammetry and UV-visible spectroscopy.Our results show that the para-phenylenediamine at low concentration gives red blood cells temporary resistance (30 min) against hemolysis in hypotonic environment with 4.5 g/L NaCl. Spectrometry results for UVvisible and cyclic voltammetry suggest that membrane stability is due to the presence of the polymer poly-paraphenylenediamine (poly-pPD).

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“…The mechanical stability of erythrocyte membrane a good indicator of insults by vegetal substances (Sharma and Sharma, 2001;Santos et al, 2010). Then, hemolysis detection is an useful and cheap technique which can displays the effect of increasing concentrations and can to be sigmoidally related to the logarithm contact time, emphasizing the membrane stability as a biological complex to maintain its structure under stress conditions, such as oxidation, hipotonicity, pH changes, heat and in presence of osmotic active solutes (Van Ginkel and Sevanian, 1994;Sharma and Sharma, 2001;Freitas et al, 2008). Herein, none of the extracts tested caused hemolysis even at the highest concentration (200 μg/ml), suggesting that the mechanism of cytotoxicity is probably related to a more specific pathway.…”

Section: Discussionmentioning

confidence: 99%

Biological screening of extracts of Brazilian Asteraceae plants

Carvalho¹,

Machado²,

Ferreira³

et al. 2013

Afr. J. Pharm. Pharmacol.

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Natural products are a very productive source of leads for the development of medicines. Seven Brazilian Asteraceae adult plants were randomly chosen. The current study was designed to evaluate the antiprotozoal and cytotoxic activities in vitro of 21 extracts obtained. Phytochemical properties of the most active extracts also were checked. Cytotoxic activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines (HCT-116, OVCAR 8 and SF-295). The antiprotozoal activity was evaluated against Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. None of the extracts showed antiprotozoal activity. However, 76% of the extracts displayed moderate to high in vitro cytotoxic activities against human cancer cell which suggests that they are a promising source of anticancer compound, since none of the extracts showed hemolytic activity. Terpenoids, flavonoids, saponins, tannins, besides other compound classes, were identified and may be responsible for their antitumor activity. Cytotoxic assays indicate the anticancer potential of Asteraceae species from Brazil.

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Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes

Cited by 65 publications

References 16 publications

Antimicrobial and Antihaemolytic Activities of Crude Extracts of Some Commonly Used Tea and Coffee in Nigeria

Antimicrobial and Antihaemolytic Activities of Crude Extracts of Some Commonly Used Tea and Coffee in Nigeria

Chemical and Biological Analyses of the Role of the Poly- Para- Phenylenediamine in the Erythrocytes Resistance in Hypotonic Environment

Biological screening of extracts of Brazilian Asteraceae plants

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