Chemical and Biological Analyses of the Role of the Poly- Para- Phenylenediamine in the Erythrocytes Resistance in Hypotonic Environment

Abstract

potential scan rate of 200 mVs-1, two voltametric waves on the platen; which corresponds to two successive and reversible processes to a single electron, leading to the formation of a diamine [24].Moreover, the genotoxicity studies in vitro have shown that pPD, like several other aromatic amines which belong to the aniline family can react with DNA and cause breaks . Picardo et al. have shown that pPD at high concentrations and for long exposure time induced cell cycle block and toxicity, but at low concentrations (10 µg/mL)and for shorter periods exposure (30 min) increased keratinocyte proliferation [39]. Habti et al. [6] have shown that pPD at low concentrations (5µg/mL) and for 2h exposure time gives RBC temporary resistance to hemolysis in hypotonic blend, the mechanism remains unexplained till now [6]. The aim of our study is to contribute to elucidate the molecular mechanism responsible for this temporary resistance. Material and Methods Collection of human blood sampleBlood samples from volunteer donors, and healthy aged 25-35 years were collected in tubes of 7 mL containing EDTA. Blood donors were nonsmokers and had no history of allergic reaction to pPD or AbstractThe high chemical and biological reactivity of para-phenylenediamine (pPD) has been illustrated in several studies, including polymerization genotoxicity and oxidative stress at the level of the plasma membrane cells. However, its reactivity with red blood cells (RBC) was rarely explored.The aim of this study is to help expound the molecular actions of para-phenylenediamine in hypotonic environment of human red blood cells by conducting biological tests for the globular resistance and physicochemical cyclic voltammetry and UV-visible spectroscopy.Our results show that the para-phenylenediamine at low concentration gives red blood cells temporary resistance (30 min) against hemolysis in hypotonic environment with 4.5 g/L NaCl. Spectrometry results for UVvisible and cyclic voltammetry suggest that membrane stability is due to the presence of the polymer poly-paraphenylenediamine (poly-pPD).