Influence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strains

Pereira, Ana Flávia Marques; Albano, Mariana; Alves, Fernanda; Andrade, Bruna Fernanda Murbach Teles; Furlanetto, Alessandra; Rall, Vera Lúcia Mores; Santos, Lucilene Delazari dos; Orsi, Ricardo de Oliveira; Júnior, Ary Fernandes

doi:10.1016/j.micpath.2020.104011

Cited by 31 publications

(8 citation statements)

References 26 publications

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“…A previous study that evaluated the activity of commercially available apitoxin against oral bacteria such as Enterococcus faecalis and Streptococcus salivarius found MIC values between 20 and 40 µg/mL [18], very similar to the results observed in L. monocytogenes in this study. Additionally, a recent study found an MIC of 7.2 µg/mL in strains of Gram-positive Staphylococcus aureus bacteria [12]. In the same way, Picoli et al [23] observed that S. aureus had lower MIC values (6-7 µg/mL) than Gram-negative Escherichia coli (40-42.5 µg/mL) and Pseudomonas aeruginosa (65-70 µg/mL) bacteria for the AMP of bee venom melittin.…”

Section: Discussionmentioning

confidence: 85%

“…The water-soluble peptide melittin from honeybee venom is one of these promising AMPs. Melittin has demonstrated both antimicrobial and antiviral activity in in vitro studies [12,13]. Melittin is a 26 amino acid cationic linear peptide with an N-terminal hydrophobic region, a C-terminal hydrophilic region, and asymmetrical distribution of polar and nonpolar amino acid residues.…”

Section: Introductionmentioning

confidence: 99%

“…Melittin exposes membrane phospholipids through pore formation to the catalytic site of phospholipase A2 [14]. Although the antimicrobial properties of melittin have been studied in depth, only limited studies have evaluated the antimicrobial ability of apitoxin, and very few strains were included [12,18]. It is therefore necessary to determine whether the apitoxins obtained in different geographic locations and tested in different studies show similar inhibition values.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Five Apitoxins from Apis mellifera on Two Common Foodborne Pathogens

et al. 2020

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Antimicrobial resistance is one of today’s major public health challenges. Infections caused by multidrug-resistant bacteria have been responsible for an increasing number of deaths in recent decades. These resistant bacteria are also a concern in the food chain, as bacteria can resist common biocides used in the food industry and reach consumers. As a consequence, the search for alternatives to common antimicrobials by the scientific community has intensified. Substances obtained from nature have shown great potential as new sources of antimicrobial activity. The aim of this study was to evaluate the antimicrobial activity of five bee venoms, also called apitoxins, against two common foodborne pathogens. A total of 50 strains of the Gram-negative pathogen Salmonella enterica and 8 strains of the Gram-positive pathogen Listeria monocytogenes were tested. The results show that the minimum inhibitory concentration (MIC) values were highly influenced by the bacterial genus. The MIC values ranged from 256 to 1024 µg/mL in S. enterica and from 16 to 32 µg/mL in L. monocytogenes. The results of this study demonstrate that apitoxin is a potential alternative agent against common foodborne pathogens, and it can be included in the development of new models to inhibit the growth of pathogenic bacteria in the food chain.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Five Apitoxins from Apis mellifera on Two Common Foodborne Pathogens

et al. 2020

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“…Melittin (a 26-amino acid peptide) is the main biologically active component in BV, which plays an important role in inducing reactions associated with bee stings (100) and it. Melittin and isoforms were also identified using the LC-MS ESI-QTOF system (225)(226)(227)(243)(244)(245)(246), SDS-PAGE (213), and HPLC (100,212).…”

Section: Compoundsmentioning

confidence: 99%

Processing Technologies for Bee Products: An Overview of Recent Developments and Perspectives

Luo

Dong

et al. 2021

Front. Nutr.

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Increased demand for a more balanced, healthy, and safe diet has accelerated studies on natural bee products (including honey, bee bread, bee collected pollen royal jelly, propolis, beeswax, and bee venom) over the past decade. Advanced food processing techniques, such as ultrasonication and microwave and infrared (IR) irradiation, either has gained popularity as alternatives or combined with conventional processing techniques for diverse applications in apiculture products at laboratory or industrial scale. The processing techniques used for each bee products have comprehensively summarized in this review, including drying (traditional drying, infrared drying, microwave-assisted traditional drying or vacuum drying, and low temperature high velocity-assisted fluidized bed drying), storage, extraction, isolation, and identification; the assessment methods related to the quality control of bee products are also fully mentioned. The different processing techniques applied in bee products aim to provide more healthy active ingredients largely and effectively. Furthermore, improved the product quality with a shorter processing time and reduced operational cost are achieved using conventional or emerging processing techniques. This review will increase the positive ratings of the combined new processing techniques according to the needs of the bee products. The importance of the models for process optimization on a large scale is also emphasized in the future.

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“…Bee venom (BV) includes a complex mixture of peptides, enzymes, lipids, and bioactive amines, and the accumulating body of data indicates its wide variety of pharmaceutical properties [1]. Accordingly, the BV remedy has been developed to deal with various sicknesses, including inflammation, cancers, microbial diseases, and neurodegenerative diseases [1][2][3][4]. Recently, BV has been used as a topical treatment for atopic dermatitis and acne because of its anti-inflammatory, anti-ageing [5,6], and anti-bacterial properties [7].…”

Section: Introductionmentioning

confidence: 99%

Cosmetic Applications of Bee Venom

El-Wahed

Khalifa

Elashal

et al. 2021

Toxins

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Bee venom (BV) is a typical toxin secreted by stingers of honeybee workers. BV and BV therapy have long been attractive to different cultures, with extensive studies during recent decades. Nowadays, BV is applied to combat several skin diseases, such as atopic dermatitis, acne vulgaris, alopecia, vitiligo, and psoriasis. BV is used extensively in topical preparations as cosmetics and used as dressing for wound healing, as well as in facemasks. Nevertheless, the safety of BV as a therapeutic choice has always been a concern due to the immune system reaction in some people due to BV use. The documented unfavorable impact is explained by the fact that the skin reactions to BV might expand to excessive immunological responses, including anaphylaxis, that typically resolve over numerous days. This review aims to address bee venom therapeutic uses in skin cosmetics.

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Influence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strains

Cited by 31 publications

References 26 publications

Antimicrobial Activity of Five Apitoxins from Apis mellifera on Two Common Foodborne Pathogens

Antimicrobial Activity of Five Apitoxins from Apis mellifera on Two Common Foodborne Pathogens

Processing Technologies for Bee Products: An Overview of Recent Developments and Perspectives

Cosmetic Applications of Bee Venom

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