Influence of anabolic combinations of an androgen plus an estrogen on biochemical pathways in bovine uterine endometrium and ovary

Becker, Christiane; Riedmaier, Irmgard; Reiter, Martina; Tichopád, Aleš; Groot, M.J.; Stolker, A.A.M.; Pfaffl, Michael W.; Nielen, M.F.W.; Meyer, H. H. D.

doi:10.1016/j.jsbmb.2011.01.007

Cited by 23 publications

(32 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nine genes could be identified as significantly regulated in uterus horn and 12 genes in uterus corpus, whereas the expression of 4 genes, namely AR, BMF4, Caspase 3 and complement factor C7, were influenced in both regions. [26] Comparing these results with those published by Reiter et al, it can be observed, that AR and Caspase 3 were also significantly regulated by Finaplix W indicating that these genes could act as first biomarkers in uterine tissue for treatment with Trenbolone Acetate. [25] Regarding the results obtained in ovary, where 22 genes were significantly regulated, it can be concluded that ovary will be a more potential organ for the identification of gene expression changes in order to find biomarkers for the abuse of anabolic steroids.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tsupporting

confidence: 68%

“…[8] These methods were already successfully used in bovine uterus, ovary, vaginal smear, liver and blood to visualize a separation between untreated controls and treated animals. [22,26,27,[30][31][32] These results show that the analysis of gene expression changes with biostatistical pattern-recognition methods will be a promising approach to identifying a biomarker pattern to screen for the misuse of anabolic agents in cattle.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 91%

“…[25] Regarding the results obtained in ovary, where 22 genes were significantly regulated, it can be concluded that ovary will be a more potential organ for the identification of gene expression changes in order to find biomarkers for the abuse of anabolic steroids. [26] Another new approach in the same animal trial was the quantification of gene expression in vaginal smear containing vaginal epithelial cells. This matrix can be taken in a non-invasive form from the living individual and due to the direct hormone responsiveness of the vaginal epithelium, expression changes of different genes, especially of factors involved in keratinization, cell growth, and apoptosis could be expected.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 99%

See 2 more Smart Citations

The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

Riedmaier

Pfaffl

Meyer

2012

Drug Testing and Analysis

View full text Add to dashboard Cite

The use of growth-promoting agents in food-producing animals is forbidden in the European Union (EU). Therefore a strict control programme has been developed, detecting residues of all known growth-promoting agents using chromatographical methods in combination with mass spectrometry or immunoassays. New designed xenobiotic substances or hormone cocktails are difficult to identify with these methods and therefore the development of new sensitive test methods is important.A promising indirect approach is the detection of physiological effects of the administered growth promoters on the molecular level using 'omic' technologies. The analysis of the transcriptome on mRNA and miRNA level and thereby identifying biomarkers for the use of anabolic agents is one possible strategy for developing a new screening method. This paper describes the technologies available for gene expression profiling and summarizes the efforts made in the analysis of the transcriptome in order to identify potential gene expression biomarkers for the use of growth promoters in cattle.

show abstract

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tsupporting

confidence: 68%

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 91%

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 99%

See 1 more Smart Citation

The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

Riedmaier

Pfaffl

Meyer

2012

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Other researchers have reported similar problems when using a combination of estrogenic and androgenic drugs in bovine animals. 34,39 For the 6-month-old female, four different steroids were administered (DHA, MPA, EB, and DROM), reaching a total dose per compound of 250 mg. Whether due to the higher estrogenic dose or the difference of age, the changes in the peptide concentration were clearer in this prepubertal animal ( Figure 5).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals

Regal

Blokland

Fente

et al. 2014

J. Agric. Food Chem.

View full text Add to dashboard Cite

The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.

show abstract

“…In females, uterus, ovary, mammary gland, and vaginal epithelial cells will be of interest. All these organs express all three types of steroid receptors whereas in uterus, mammary gland and vaginal epithelial cells ERα and in ovary ERβ is the prevalent isoform of the estrogen receptor 21–26. In the male organism, testis and prostate will be of major interest.…”

Section: Tissue Distribution Of Steroid Hormone Receptorsmentioning

confidence: 99%

The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones

Riedmaier

Pfaffl

Meyer

2011

Drug Testing and Analysis

View full text Add to dashboard Cite

The abuse of anabolic steroid hormones in human sports and animal husbandry is an ubiquitous problem and therefore a tight control program in both areas is very important. Within these control programs, hormone residues are detected by immunoassays or chromatographical methods in combination with mass spectrometry. With these methods, all known substances can be detected; yet new xenobiotic growth promoters and new ways of application are difficult to detect. Therefore it is important to develop new sensitive screening methods to enable an efficient control for misused anabolic substances. The detection of their physiological action is a promising approach. Anabolic steroid hormones directly influence the expression of specific genes and thus the analysis of the transcriptome of different target tissues and matrices is of great interest. This review describes our recent efforts made concerning the analysis of gene expression changes in different tissues, different species and under different anabolic treatments.

show abstract

Influence of anabolic combinations of an androgen plus an estrogen on biochemical pathways in bovine uterine endometrium and ovary

Cited by 23 publications

References 43 publications

The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals

The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones

Contact Info

Product

Resources

About