Influence of age on mitochondrial enzyme levels in Drosophila

Massie, Harold R.; Kogut, Kelly A.

doi:10.1016/0047-6374(87)90072-8

Mechanisms of Ageing and Development

1987

DOI: 10.1016/0047-6374(87)90072-8

|View full text |Cite

Influence of age on mitochondrial enzyme levels in Drosophila

Harold R. Massie

Kelly A. Kogut

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1994

2008

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 24 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Mitochondria and ageing in Drosophila

Morrow

Tanguay

2008

Biotechnology Journal

View full text Add to dashboard Cite

Studies in different organisms have revealed that ageing is a complex process involving a tight regulation of gene expression. Among other features, ageing organisms generally display an increased oxidative stress and a decreased mitochondrial function. The increase in oxidative stress can be attributable to reactive oxygen species, which are mainly produced by mitochondria as a by-product of energy metabolism. Consistent with these data, mitochondria have been suggested to play a significant role in lifespan determination. The fruitfly Drosophila melanogaster is a well-suited organism to study ageing as it is relatively short-lived, mainly composed of post-mitotic cells, has sequenced nuclear and mitochondrial genomes, and multiple genetic tools are available. It has been used in genome-wide studies to unveil the molecular signature of ageing, in different feeding and dietary restriction protocols and in overexpression and down-regulation studies to examine the effect of specific compounds or genes/proteins on lifespan. Here we review the various features linking mitochondria and ageing in Drosophila melanogaster.

show abstract

Mitochondria and ageing in Drosophila

Morrow

Tanguay

2008

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

Changes in the respiratory chain complexes activities and in the mitochondrial DNA content during ageing in D. subobscura

Morel

Mazet

Touraille

et al. 1995

Mechanisms of Ageing and Development

View full text Add to dashboard Cite

Cellular and Subcellular Localization of Hexokinase, Glutamate Dehydrogenase, and Alanine Aminotransferase in the Honeybee Drone Retina

Veuthey

Tsacopoulos

Ruiz

et al. 1994

Journal of Neurochemistry

View full text Add to dashboard Cite

Subcellular localization of hexokinase in the honeybee drone retina was examined following fractionation of cell homogenate using differential centrifugation. Nearly all hexokinase activity was found in the cytosolic fraction, following a similar distribution as the cytosolic enzymatic marker, phosphoglycerate kinase. The distribution of enzymatic markers of mitochondria (succinate dehydrogenase, rotenone‐insensitive cytochrome c reductase, and adenylate kinase) indicated that the outer mitochondrial membrane was partly damaged, but their distributions were different from that of hexokinase. The activity of hexokinase in purified suspensions of cells was fivefold higher in glial cells than in photoreceptors. This result is consistent with the hypothesis based on quantitative 2‐deoxy[3H]glucose autoradiography that only glial cells phosphorylate significant amounts of glucose to glucose‐6‐phosphate. The activities of alanine aminotransferase and to a lesser extent of glutamate dehydrogenase were higher in the cytosolic than in the mitochondrial fraction. This important cytosolic activity of glutamate dehydrogenase was consistent with the higher activity found in mitochondria‐poor glial cells. In conclusion, this distribution of enzymes is consistent with the model of metabolic interactions between glial and photoreceptor cells in the intact bee retina.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Influence of age on mitochondrial enzyme levels in Drosophila

Cited by 3 publications

References 24 publications

Mitochondria and ageing in Drosophila

Mitochondria and ageing in Drosophila

Changes in the respiratory chain complexes activities and in the mitochondrial DNA content during ageing in D. subobscura

Cellular and Subcellular Localization of Hexokinase, Glutamate Dehydrogenase, and Alanine Aminotransferase in the Honeybee Drone Retina

Contact Info

Product

Resources

About