Changes in the respiratory chain complexes activities and in the mitochondrial DNA content during ageing in D. subobscura

Morel, F.; Mazet, Françoise; Touraille, Sylvie; Alziari, Serge

doi:10.1016/0047-6374(95)01653-8

Cited by 26 publications

(12 citation statements)

References 30 publications

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“…A reduction in the cytochrome c oxidase activity of complex IV has been hypothesized to increase the residence times of electrons upstream in the chain, increase the probability of electrons leaking from the chain to form superoxide, and decrease mitochondrial efficiency (Sohal & Sohal, 1991; Sohal & Dubey, 1994). Consistent with this idea, inhibition of complex IV cytochrome c oxidase activity has been shown to increase the rate of mitochondrial H 2 O 2 production (Morel et al ., 1995; Miwa et al ., 2003; Ferguson et al ., 2005).…”

Section: Introductionmentioning

confidence: 74%

Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild‐caught Drosophila simulans

Melvin

Ballard

2006

Aging Cell

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SummaryLifespans of organisms vary greatly even among individuals of the same species. Under the framework of the free oxygen radical theory of aging, it is predicted that variation in individual lifespan within a species will correlate with variation in the accumulation of oxidative damage to cell components from reactive oxygen species. In this study we test the hypothesis that variation in survival of three wildcaught Drosophila simulans fly lines (HW09, NC48 and MD106) correlates with three key aspects of mitochondrial bioenergetics. The rank order of median survival was HW09 > MD106 > NC48. Young HW09 flies (11-18 days) had (i) highest ADP:O (quantity of oxygen consumed by mitochondria when provided with a quantity of ADP) when metabolizing both electron transport chain complex I and complex III substrates; (ii) lowest rate of mitochondrial hydrogen peroxide production from complex III; and (iii) highest cytochrome c oxidase activity from complex IV. Rate of hydrogen peroxide production increased and cytochrome c oxidase activity decreased in all lines in the age range 11-25 days. This is the first study to correlate natural variation in organism survival with natural variation in mitochondrial bioenergetics.

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Section: Introductionmentioning

confidence: 74%

Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild‐caught Drosophila simulans

Melvin

Ballard

2006

Aging Cell

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“…There was also no apparent decline in metabolic rate from middle to old age. Such a decline might be predicted based on data obtained from D. subobscura (36), showing significant decreases in the levels of several key respiratory enzymes with age. One reason such a decline was not seen here may be because metabolic rates were measured on relatively inactive flies.…”

Section: Discussionmentioning

confidence: 99%

Selected Contribution: Long-livedDrosophila melanogasterlines exhibit normal metabolic rates

Voorhies¹,

Khazaeli²,

Curtsinger³

2003

Journal of Applied Physiology

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The use of model organisms, such as Drosophila melanogaster, provides a powerful method for studying mechanisms of aging. Here we report on a large set of recombinant inbred (RI) D. melanogaster lines that exhibit approximately a fivefold range of average adult longevities. Understanding the factors responsible for the differences in longevity, particularly the characteristics of the longest-lived lines, can provide fundamental insights into the mechanistic correlates of aging. In ectothermic organisms, longevity is often inversely correlated with metabolic rate, suggesting the a priori hypothesis that long-lived lines will have low resting metabolic rates. We conducted approximately 6000 measurements of CO2 production in individual male flies aged 5, 16, 29, and 47 days postemergence and simultaneously measured the weight of individual flies and life spans in populations of each line. Even though there was a wide range of longevities, there was no evidence of an inverse relationship between the variables. The increased longevity of long-lived lines is not mediated through reduction of metabolic activity. In Drosophila, it is possible to both maintain a normal metabolic rate and achieve long life. These results are evaluated in the context of 100 years of research on the relationship between metabolic rate and life span.

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“…A decrease in cytochrome c oxidase (complex IV) activity is an alternative mechanism whereby electron flow within the respiratory chain could be impaired. Indeed, a decline in complex IV activity is the most consistently reported age-related alteration in the electron transport systems of both insect and mammalian mitochondria [13][14][15][16]24]. However, the conclusion that there is any overt effect of aging on the electron transport system has been questioned recently [26], because many studies of mitochondrial respiration omitted crucial controls, such as the measurement of citrate synthase activity.…”

Section: Discussionmentioning

confidence: 99%

“…In mammals, including humans, defects in the electron transport chain clearly contribute to the aetiology of several disease states; however, investigations of whether similar defects occur during normal aging have yielded inconsistent results [12][13][14][15][16][17][18][19][20]. Similarly, in insects, there is a considerable amount of contradictory evidence, with some studies reporting a decline in oxygen consumption and/or alterations in electron transport chain complex activities with increasing age, whereas others report no significant age-related alterations [21][22][23][24][25]. Although several possibilities have been raised to explain such discordant findings, the most frequently implicated are age-associated increases in mitochondrial fragility, damage during isolation and inadequate verification of mitochondrial purity.…”

Section: Introductionmentioning

confidence: 99%

Age-associated decline in mitochondrial respiration and electron transport in Drosophila melanogaster

Ferguson

Mockett

Shen

et al. 2005

Biochemical Journal

185

184

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The principal objective of the present study was to identify specific alterations in mitochondrial respiratory functions during the aging process. Respiration rates and the activities of electron transport chain complexes were measured at various ages in mitochondria isolated from thoraces of the fruit fly, Drosophila melanogaster, which consist primarily of flight muscles. The rates of state 3 respiration (ADP-stimulated), RCRs (respiratory control ratios) and uncoupled respiration rates decreased significantly as a function of age, using either NAD+- or FAD-linked substrates; however, there were no differences in state 4 respiration (ADP-depleted) rates. There was also a significant age-related decline in the activity of cytochrome c oxidase (complex IV), but not of the other mitochondrial oxidoreductases examined. Exposure of mitochondria isolated from young flies to low doses of KCN or NaAz (sodium azide), complex IV inhibitors, decreased cytochrome c oxidase activity and increased the production of H2O2. Collectively, these results support the hypothesis that impairment of mitochondrial respiration may be a causal factor in the aging process, and that such impairment may result from and contribute to increased H2O2 production in vivo.

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Changes in the respiratory chain complexes activities and in the mitochondrial DNA content during ageing in D. subobscura

Cited by 26 publications

References 30 publications

Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild‐caught Drosophila simulans

Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild‐caught Drosophila simulans

Selected Contribution: Long-livedDrosophila melanogasterlines exhibit normal metabolic rates

Age-associated decline in mitochondrial respiration and electron transport in Drosophila melanogaster

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