Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects

Manríquez, Víctor; Gutierrez, Amparo; Morales, Alexis; Brito, Roberto; Pavez, Mónica; Sapunar, J; Fonseca, Luís; Molina, Víctor; Ortiz, Eugenia; Barra, María Inés; Reimer, Camila; Charles, M St.; Schneider, Connie; Cerda, Álvaro

doi:10.1038/s41366-020-00654-9

Cited by 8 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results show that HAd36 seropositive subjects present higher expression of PPARγ and miR-17 (pro-adipogenic), but lower expression of miR-155 (anti-adipogenic), which may favor a proadipogenic state in these subjects. 25 Therefore, the role of miRNAs in the epigenetic regulation of adipogenesis in HAd36 infection represents a new line of research that will continue to be developed to clarify the underlying mechanisms of this viral infection.…”

Section: Discussionmentioning

confidence: 99%

Presence of Adenovirus-36 DNA in Adipose Tissue of Women: Relationship with Adipocyte Morphology and the Expression of C/EBPβ and HIF-1α

Barrera-Alcocer

García-Benavides

Muñoz‐Valle

et al. 2021

DMSO

View full text Add to dashboard Cite

Background Human adenovirus 36 (HAd36) infection has been associated with obesity. Experiments using 3T3-L1 adipocyte cultured cells and human adipose stem cells (hASCc) have shown that HAd36 stimulates the expression of genes implicated in cell differentiation and increased lipid accumulation. The presence of HAd36 in adipose tissue of overweight and obese women has also been confirmed. This study aims to analyze the presence of HAd36 DNA in the adipose tissue of women undergoing surgery for weight reduction and its relationship with obesity through changes in adipocyte morphology as well as the expression of C/EBPβ and HIF-1α. Methods Fifty-two subcutaneous adipose tissue biopsies were collected. The anthropometric parameters measured were weight, height, skin folds, body circumferences, and body fat percentage. Biochemical measures were performed for glucose, cholesterol, triglycerides, cholesterol HDL-c, and LDL-c. The presence of HAd36 DNA was performed by conventional PCR. Adipocyte morphology was analyzed in H&E-stained sections using ImageJ/Fiji software. The expression of genes C/EBPβ, HIF-1α and β-actin was determined using TaqMan probes. Results HAd36 DNA was detected in 31% of adipose tissue samples. The presence of viral DNA was not significantly associated with anthropometric, clinical, or metabolic measurements, or with changes in adipose tissue morphology. The levels of mRNA expression for C/EBPβ and HIF-1α did not show significant differences between positive and negative samples for HAd36 (p>0.05). Conclusion The presence of HAd36 DNA in adipose tissue was identified, but it was not related to morphological changes of adipocytes, or the expression of C/EBPβ and HIF-1α. Further studies are needed to confirm these findings.

show abstract

Section: Discussionmentioning

confidence: 99%

Presence of Adenovirus-36 DNA in Adipose Tissue of Women: Relationship with Adipocyte Morphology and the Expression of C/EBPβ and HIF-1α

Barrera-Alcocer

García-Benavides

Muñoz‐Valle

et al. 2021

DMSO

View full text Add to dashboard Cite

show abstract

“…The study population analyzed in this work was obtained from a previous work of our research group [15]. In that work we reported that obese individuals previously infected by Ad-36 (Ad-36[+]; n = 29) were associated with lower concentration of circulating leptin (p = 0.032), higher percentage of fat mass (p = 0.045), and a higher expression of the adipogenic master gene PPARG (p = 0.008) (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Epigenetic mechanisms, which include DNA methylation, histone post-translational modi cations, ATPdependent chromatin modi cation and the action of non-coding RNAs, as microRNAs (miRNAs) [15][16][17] or non-long coding RNAs (lncRNAs) [18][19][20][21] are involved in the development and maintenance of various conditions and pathologies, such as adipogenesis and obesity [22][23][24][25][26]. In a rst approach to answer this question, we previously evaluated the in uence of Ad-36 seropositivity on the expression of adipogenic microRNAs in VAT from obese subjects showing a dysregulation of adipogenic and anti-adipogenic miRNAs according previous exposition to Ad-36 [15], suggesting a role in the long-term maintenance of the pro-adipogenic state. In this way, it is interesting to evaluate the role of another and more complex type of non-coding RNA as the lncRNAs, which are non-coding RNAs longer than 200 nt [27,28] with various and vital functions in cell biology and pathophysiological processes, such as adipose tissue biology and development of obesity [19,29].…”

Section: Introductionmentioning

confidence: 99%

Adenovirus 36 seropositivity influences the expression of anti-adipogenic lncRNAs GAS5 and MEG3 in adipose tissue obtained from subjects with obesity

Cerda,

Manriquez,

Brito

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background Adenovirus 36 (Ad-36) promotes adipogenesis and the development of human obesity. Induction of PPARγ by Ad-36 seems to have a central role in maintenance of adipogenic status. There is limited information about epigenetic mechanisms contributing to this process in human adipose tissue. This study evaluated the expression of lncRNAs (ADINR, GAS5 and MEG3) and miRNAs ((miR-18a and miR-140) involved in adipogenic process in visceral adipose tissue (VAT) of subjects with obesity with previous Ad-36 infection (seropositive) and unexposed (seronegative) obese subjects.Methods Obese individuals were grouped according to seroconversion against Ad-36 (Seropositive: Ad-36[+], n = 29; and Seronegative: Ad-36[-], n = 28). Additionally, a group of lean controls (n = 17) was selected to compare with the obese group. The Ad-36 serology was carried out by ELISA. Biopsies of VAT were obtained during an elective and clinically indicated surgery (bariatric or cholecystectomy). RNA extraction from VAT was performed and the expression of PPARG and non-coding RNAs was evaluated by qPCR.Results Ad-36[+] individuals had lower expression of anti-adipogenic lncRNAs GAS5 (p = 0.016) and MEG3 (p = 0.035) compared with Ad-36[-] obese subjects. Ad-36[+] subjects also presented increased expression of the adipogenic miRNA miR-18a (p = 0.042), which has been reported to be modulated by GAS5 through a RNA sponging mechanism during adipogenic differentiation. Additionally, an inverse correlation of GAS5 with PPARG expression was observed (r= -0.917, p = 0.01).Conclusion Our results suggest the participation of non-coding RNAs implicated in adipogenic process in the long-term maintenance of adipogenic status associated with previous Ad-36 infection, probably through the GAS5/miR-18a axis.

show abstract

“…In addition, a decrease in anti-adipogenic hsa-miR-155 was observed. AdV36 infection was found to positively affect adipose cell differentiation, with proliferation and dysregulation of hsa-miR-17 and hsa-miR-155 caused the increased adipogenic status in AdV36-serotype-positive individuals [ 70 ].…”

Section: Mirna Pathway Dysregulation In Response To the Entry Of Geno...mentioning

confidence: 99%

miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy

Savenkova

Makarova

Shalik

et al. 2022

IJMS

View full text Add to dashboard Cite

Gene therapy is widely used to treat incurable disorders and has become a routine procedure in clinical practice. Since viruses can exhibit specific tropisms, effectively penetrate the cell, and are easy to use, most gene therapy approaches are based on viral delivery of genetic material. However, viral vectors have some disadvantages, such as immune response and cytotoxicity induced by a disturbance of cell metabolism, including miRNA pathways that are an important part of transcription regulation. Therefore, any viral-based gene therapy approach involves the evaluation of side effects and safety. It is possible for such effects to be caused either by the viral vectors themselves or by the delivered genetic material. Many gene therapy techniques use non-coding RNA delivery as an effective agent for gene expression regulation, with the risk of cellular miRNA pathways being affected due to the nature of the non-coding RNAs. This review describes the effect of viral vector entry and non-coding RNA delivery by these vectors on miRNA signaling pathways.

show abstract

Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects

Cited by 8 publications

References 44 publications

Presence of Adenovirus-36 DNA in Adipose Tissue of Women: Relationship with Adipocyte Morphology and the Expression of C/EBPβ and HIF-1α

Presence of Adenovirus-36 DNA in Adipose Tissue of Women: Relationship with Adipocyte Morphology and the Expression of C/EBPβ and HIF-1α

Adenovirus 36 seropositivity influences the expression of anti-adipogenic lncRNAs GAS5 and MEG3 in adipose tissue obtained from subjects with obesity

miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy

Contact Info

Product

Resources

About