Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution

Ivanov, Yuri D.; Kozlov, Andrey F.; Galiullin, Rafael A.; Tatur, Vadim Yu.; Ziborov, Vadim S.; Usanov, Sergey A.; Pleshakova, Tatyana O.

doi:10.3390/separations5020029

Cited by 4 publications

(6 citation statements)

References 12 publications

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“…It is to be noted that the generation of charge during the flow of water and aqueous solutions along various surfaces [8], as well as changes in the physicochemical properties of water upon its flowing through polymer injectors [9], were long and widely discussed in the literature. In our previous paper [10], we demonstrated that negative pulsed voltage applied to parallel metal plates, between which an injector of the AFM-based fishing system was located, led to an increase in the efficiency of charge generation in the injector. At the same time, we demonstrated that an external AC electric field (with 50 Hz mains frequency, which is often present in biosensor devices) caused an increase in efficiency of charge generation in water flowing through an injector at a low-grade fever temperature (38 • C) [11]; this temperature is higher than that of phase transition of water related with heat capacity (36.6 • C, [9]) and corresponds to a pathology in human.…”

Section: Introductionmentioning

confidence: 96%

“…The influence of external low-frequency (50 Hz) AC electric field, applied to the injector of the system for AFM-based fishing, has also been studied. Similar to References [10,11,16], this field has been induced by negative AC voltage applied to electrodes, between which the injector was located. The temperature of T = 35 • C was selected due to the following considerations.…”

Section: Introductionmentioning

confidence: 97%

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Influence of Chip Materials on Charge Generation in Flowing Solution in Nanobiosensors

et al. 2019

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Nowadays, nanobiosensors are being intensively developed due to the potential possibilities of their use for early diagnosis of diseases. This interest is enhanced by the fact that, as is known, a pathological process at an early stage is characterized by the appearance of marker proteins at very low (10−15 M and lower) concentrations in blood. Highly-sensitive nanobiosensor systems (including those based on an atomic force microscope, AFM) allows one to detect proteins at such low concentrations. The influence of the charge generated in the analyte solution flowing through the biosensor injector into the measuring cell during measurements is considered to be an important factor conditioning such a high detection sensitivity. In the present study, it was demonstrated that the presence of an AFM chip (made of mica and graphite) near the nozzle of the injector supplying an analyte solution into the measuring cell of the AFM-based fishing system (AFM-based nanobiosensors) causes an increase in charge generation upon the injection of the solution. Moreover, the influence of polymer materials (which are widely used in nanobiosensors) and communications on charge generation in the flow-based section of AFM-based nanobiosensors was studied. A stimulating influence of a low (femtomolar) concentration of proteins on the charge generation in polymeric injectors of flow-based nanobiosensors was demonstrated. Besides, a stimulating influence of an external low-frequency AC electric field on the charge generation in the nanobiosensor injector was found. Measurements were carried out in the temperature range corresponding to the physiological temperature (35 °C).

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Influence of Chip Materials on Charge Generation in Flowing Solution in Nanobiosensors

et al. 2019

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“…With the use of various mechanisms, including those with a radiation-based principle of operation, such interaction can be observed in living cells, as well as in an organism, featuring central nervous system, whose life-sustaining activity is also accompanied by other factors exciting spin transition of water, e.g., electric pulses generated during the functioning of blood circulatory and nervous system due to a triboelectric effect [27,28]. It should be noted that the possibility of enhancement of the parameters of radiation from an enzyme system by means of its stimulation with electric pulses was demonstrated with the example of another heme-containing peroxidase system [29].…”

Section: Discussionmentioning

confidence: 99%

The Registration of a Biomaser-Like Effect in an Enzyme System with an RTM Sensor

Ivanov

Malsagova

Vesnin³

et al. 2019

Journal of Sensors

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The concentration dependence of a microwave frequency radiation from a solution of a functioning enzyme system (ES) (with the example of cytochrome P450 BM3 (CYP102A1) system during lauric acid (LA) hydroxylation) has been studied with a radiothermometric sensor. Registration of the radiation from the enzyme solution has been performed in the frequency range from 3.4 to 4.2 GHz at the enzyme concentrations from 10−10 М to 10−6 М. It has been demonstrated that the catalysis of LA hydroxylation in a reconstituted CYP102A1 system is accompanied by a generation of microwave radiation over the entire range of concentrations studied. It has been found that a transition from a multipulse mode (at nanomolar enzyme concentrations from 10−10М to 10−8М) to a single-pulse mode (at micromolar enzyme concentrations from 10−7М to 10−6М) is observed. This effect is discussed on the basis of assumptions considering possible realization of biomaser-like radiation in the enzyme system. The discovered concentration-based effect of the transition of an unsynchronized pulsed radiation into a synchronized one in ES can further be used in the development of novel methods of noninvasive diagnostics of diseases, in mathematical modeling of the functioning of living systems, and in the development of next-generation quantum computers.

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“…Upon the flow of water through the plastic pipe and the tip, an electric charge was generated in it owing to a triboelectric effect [29][30][31][32][33][34][35][36]40,41]. The electric field, organized around the tip, induced charge separation in water.…”

Section: Generation Of Electric Charge and Its Separation In Water Dr...mentioning

confidence: 99%

“…The first way is the application of an external electric field [27]; it is used, for instance, for the electrophoretic separation of proteins [28]. The second way is the use of electrostatic effects occurring in an analytical system upon a motion of a liquid medium [29][30][31][32][33][34][35][36]. This effect of the occurrence of an electric field (that is, the generation of charge) in the analytical system influences its properties.…”

Section: Introductionmentioning

confidence: 99%

The Use of Excess Electric Charge for Highly Sensitive Protein Detection: Proof of Concept

Kanashenko¹,

Galiullin²,

Shumov³

et al. 2022

Electronics

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In highly sensitive bioanalytical systems intended for the detection of protein biomarkers at low and ultra-low concentrations, the efficiency of capturing target biomolecules from the volume of the analyzed sample onto the sensitive surface of the detection system is a crucial factor. Herein, the application of excess electric charge for the enhancement of transport of target biomolecules towards the sensitive surface of a detection system is considered. In our experiments, we demonstrate that an uncompensated electric charge is induced in droplets of protein-free water owing to the separation of charge in a part of the Kelvin dropper either with or without the use of an external electric field. The distribution of an excess electric charge within a protein-free water droplet is calculated. It is proposed that the efficiency of protein capturing onto the sensitive surface correlates with the sign and the amount of charge induced per every single protein biomolecule. The effect described herein can allow one to make the protein capturing controllable, enhancing the protein capturing in the desired (though small) sensitive area of a detector. This can be very useful in novel systems intended for highly sensitive detection of proteins at ultra-low (≤10−15 M) concentrations.

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Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution

Cited by 4 publications

References 12 publications

Influence of Chip Materials on Charge Generation in Flowing Solution in Nanobiosensors

Influence of Chip Materials on Charge Generation in Flowing Solution in Nanobiosensors

The Registration of a Biomaser-Like Effect in an Enzyme System with an RTM Sensor

The Use of Excess Electric Charge for Highly Sensitive Protein Detection: Proof of Concept

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