The Registration of a Biomaser-Like Effect in an Enzyme System with an RTM Sensor

Abstract

The concentration dependence of a microwave frequency radiation from a solution of a functioning enzyme system (ES) (with the example of cytochrome P450 BM3 (CYP102A1) system during lauric acid (LA) hydroxylation) has been studied with a radiothermometric sensor. Registration of the radiation from the enzyme solution has been performed in the frequency range from 3.4 to 4.2 GHz at the enzyme concentrations from 10−10 М to 10−6 М. It has been demonstrated that the catalysis of LA hydroxylation in a reconstituted CYP102A1 system is accompanied by a generation of microwave radiation over the entire range of concentrations studied. It has been found that a transition from a multipulse mode (at nanomolar enzyme concentrations from 10−10М to 10−8М) to a single-pulse mode (at micromolar enzyme concentrations from 10−7М to 10−6М) is observed. This effect is discussed on the basis of assumptions considering possible realization of biomaser-like radiation in the enzyme system. The discovered concentration-based effect of the transition of an unsynchronized pulsed radiation into a synchronized one in ES can further be used in the development of novel methods of noninvasive diagnostics of diseases, in mathematical modeling of the functioning of living systems, and in the development of next-generation quantum computers.

AFM Imaging of Protein Aggregation in Studying the Impact of Knotted Electromagnetic Field on A Peroxidase

“…The problem of the effect of microwave radiation on enzymes is of great interest. Particularly, this concerns the power range at the level of background radiation (~10 −12 W/cm 2 ), at which the radiation in enzyme systems is registered 29 and pathological changes in human are detected 30 . The impact of microwave radiation on proteins is determined by both the radiation power and the exposure time.…”

Passive microwave radiometry in biomedical studies

“…This effect could be explained using biomaser hypothesis. Also, the synchronized pulsed radiation could indicate the generation of qubits in a biological system [34]. The similar 0.5 C microwave emission increase in passive mode and 2 C in excitation mode was observed in a solution of horseradish peroxidase (HRP).…”

“…Microwave emission has been observed as well -in the process of hydroxylation of enzyme CYP102 A1 [34]. The 0.5-2 C increase within 10-20 s in multi-pulse mode follows the addition of cosubstrate NADPH (0.2 mM) to the system containing lauric acid (0.5 mM), and enzyme 10 À10 to 10 À8 M. A single-pulse mode (synchronized pulse radiation) at 10 À6 M concentration of enzyme was observed.…”

“…Each HRP and CYP102 A1 reaction is accompanied by the fluctuation of the protein globule with approximately the same amplitude (in the order of 0.08 nm). The microwave emission effect could be explained by rotational transitions of molecules and ions excited during catalysis (OH À , H 2 O and H 2 O 2 ) in GHz (the change in the ratio between para-and ortho-isomers of H 2 O in GHz), with increased mobility of the protein globule during the catalytic reaction [33,34].…”

“…However, recent studies show that, in many cases, non-thermal radiation of living systems in the microwave range also takes place. To better understand the nature of the phenomena several in vitro experiments on cell lines [32], enzymes [33,34] and proteins [35,37] were performed. Commercially available equipment: RTM-01-RES (http://www.mmwr.co.uk) ( Fig.…”