Influence of a Nanoporous Zirconia Implant Surface of on Cell Viability of Human Osteoblasts

Aboushelib, Moustafa N.; Osman, Essam; Jansen, Ineke D. C.; Everts, Vincent; Feilzer, Albert J.

doi:10.1111/j.1532-849x.2012.00920.x

Cited by 29 publications

(24 citation statements)

References 31 publications

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“…The SIE technique resulted in a nanoroughened (0.83 ± 0.09 μm) surface, while no structural defects were noted. 17,26 The SIE enhanced cell proliferation and growth when compared with the other surface treatments; this may be due to the new surface topography that is more uniform compared with the others, which helped in initial cell attachment and growth, clearly observed after 7 days that was in accordance with previous published papers. [27][28][29] The use of laser energy in zirconia-surface treatment was a subject of interest for researchers.…”

Section: Discussionsupporting

confidence: 88%

“…6,41 Conventional sandblasting with particles >100 μm causes surface damage and affects the properties of the Y-TZP as reported previously 13 ; thus, recommendations for zirconia surface treatment were a combination of sandblasting with small sized particle (30 μm) and a chemical conditioner that enhance the bonding to resin. 13,17 A lowpressure air abrasion method was proposed that reduces surface damage. 42 The alkaline phosphate activity was used to evaluate the differentiation of cells of the osteogenic lineage and compare the cell activity in different tested groups.…”

Section: Discussionmentioning

confidence: 99%

“…17 The osseointegration of an implant material is determined by the surface characteristics of the material, such as surface chemistry, surface charge, bulk material rigidity, and roughness. These characteristics influence the cell adhesion and proliferation to the zirconia material.…”

Section: 5005/jp-journals-10024-2234mentioning

confidence: 99%

“…A paper evaluating the influence of a nanoporous zirconia implant surfaces showed that they favored cell growth and attachment compared with the polished surface. 17 The aim of this study was to evaluate the effect of different zirconia surface treatments on cell adhesion and proliferation. The null hypotheses tested were that surface treatment has no effect on cell adhesion and proliferation on treated zirconia surface.…”

Section: 5005/jp-journals-10024-2234mentioning

confidence: 99%

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Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Nassif

Rifai

2018

The Journal of Contemporary Dental Practice

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Aim:The aim of this study was to characterize the surface of zirconia subjected to different treatments and evaluate its effect on cell adhesion and proliferation. Materials and methods:A total of 80 zirconia disks were divided into four groups (n = 20) according to the surface treatments used: group I: as-sintered (AS), no surface treatment applied; group II: abrasion treatment applied using Rocatec (ROC; 3M ESPE) system with silica-coated alumina powder of grit size 110 μm; group III: erbium, chromium:yttrium, scandium, gallium, garnet (Er, Cr:YSGG) laser (LAS; BIOLASE) was used at a frequency of 20 Hz and output power of 3 W; and group IV: specimens were subjected to the selective infiltration etching (SIE) technique. Surface characterization was evaluated for the different groups (roughness, hardness, and morphology), and cell behavior (adhesion and proliferation) was tested (α = 0.05). Results:The ROC group reported a significant increase in surface roughness (2.201 ± 0.352) and Vickers hardness (1758 ± 16.6) compared with the other surface treatments. The SIE surface-treated group reported a significantly higher number of cells (64.5 ± 2.6 and 53.5 ± 2.2 respectively) compared with the other surface-treated groups. Conclusion:The SIE is a promising surface treatment for zirconia that significantly enhances cell adhesion and osseointegration.Clinical significance: The SIE treatment of zirconia implants may help in a faster and better osseointegration.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: 5005/jp-journals-10024-2234mentioning

confidence: 99%

Section: 5005/jp-journals-10024-2234mentioning

confidence: 99%

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Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Nassif

Rifai

2018

The Journal of Contemporary Dental Practice

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“…Additionally, smooth topography material based on zirconia (Cercon) was used for comparison. Although Ti-SLA, Ti-SLActive and TiZrSLActive topographies have been investigated in vivo and in clinical practice [50][51][52][53][54][55], studies on human primary cells are still limited [56][57][58][59]. Osteoblast cells were allowed to proliferate on the investigated implant surfaces up to 10 days and evaluated by the DAPI assay ( Figure 1).…”

Section: Cell Proliferationmentioning

confidence: 99%

Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation

et al. 2017

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A primary goal in modern surface modification technology of dental implants is to achieve biocompatible surfaces with rapid but controlled healing which also allow health and longevity of implants. In order to realize all, understanding of osseointegration phenomena is crucial. Although Ti-SLA, Ti-SLActive and TiZr-SLActive surfaces have been successfully used in clinical implantology and were shown to notably reduce the primary healing time, available in vitro studies are sparse and do not concern or explore the mechanism(s) involved in human osteoblast behavior on these surfaces. Ti-SLA, Ti-SLActive, TiZr-SLActive, Ti cp, Ticer and Cercon surfaces were used. Osteoblast proliferation, cell cluster formation, morphological changes, induction of autophagy, nitric oxide (NO), reactive oxygen species/reactive nitrogen species (ROS/RNS) formation, osteocalcin (OC), bone sialoprotein (BSP) and collagen type I (Col-1) affected by various surfaces were analyzed. These surfaces induced formation of mature osteoblasts caused by elevated oxidative stress (ROS) followed by overexpression of osteoblast maturation key molecule (NO), with different intensity however. These mature osteoblasts induced upregulation of OC, BSP and Col-1, activating PI3/Akt signalling pathway resulting in autophagy, known as an important process in differentiation of osteoblast cells. Additional distinctive subpopulation identified on Ticer, Ti-SLA (after 5 days), Ti-SLActive and TiZr-SLActive surfaces (after 2 days) were forming cell clusters, essential for bone noduli formation and mineralisation. The results suggest that Ti-and TiZr-SLActive possess advanced properties in comparison with Ticer and Ti-SLA manifested as accelerated osteoblast differentiation. These effects could explain already known fast osseointegration of these surfaces in vivo.

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Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

Altmann

Karygianni

Al‐Ahmad

et al. 2017

Adv Funct Materials

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The development of long-lasting zirconia-based ceramics for implants, which are not prone to hydrothermal aging, is not satisfactorily solved. Therefore, this study is conceived as an overall evaluation screening of novel ceriastabilized zirconia-alumina-aluminate composite ceramics (ZA 8 Sr 8 -Ce11) with different surface topographies for use in clinical applications. Ceriastabilized zirconia is chosen as the matrix for the composite material, due to its lower susceptibility to aging than yttria-stabilized zirconia (3Y-TZP). This assessment is carried out on three preclinical investigation levels, indicating an overall biocompatibility of ceria-stabilized zirconia-based ceramics, both in vitro and in vivo. Long-term attachment and mineralized extracellular matrix (ECM) deposition of primary osteoblasts are the most distinct on porous ZA 8 Sr 8 -Ce11 p surfaces, while ECM attachment on 3Y-TZP and ZA 8 Sr 8 -Ce11 with compact surface texture is poor. In this regard, the animal study confirms the porous ZA 8 Sr 8 -Ce11 p to be the most favorable material, showing the highest bone-to-implant contact values and implant stability post implantation in comparison with control groups. Moreover, the microbiological evaluation reveals no favoritism of biofilm formation on the porous ZA 8 Sr 8 -Ce11 p when compared to a smooth control surface. Hence, together with the in vitro in vivo assessment analogy, the promising clinical potential of this novel ZA 8 Sr 8 -Ce11 as an implant material is demonstrated.

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Influence of a Nanoporous Zirconia Implant Surface of on Cell Viability of Human Osteoblasts

Abstract: The findings of this study suggest that a nanoporous zirconia surface favors cell growth and attachment compared to a polished surface. It was proposed that a nanoporous zirconia surface may improve clinical performance of zirconia implants.

Cited by 29 publications

References 31 publications

Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation

Assessment of Novel Long‐Lasting Ceria‐Stabilized Zirconia‐Based Ceramics with Different Surface Topographies as Implant Materials

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