Influence of a moderate physical activity intervention on red cell deformability in patients suffering from chronic obstructive pulmonary disease (COPD)

Ahmad, Basit; Ferrari, Nina; Montiel, Georgina; Bloch, Wilhelm; Raabe-Oetker, A.; Skrobala, Nina; Brixius, Klara

doi:10.1007/s10354-013-0183-7

Cited by 5 publications

(6 citation statements)

References 52 publications

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“…Along the same lines, patients with compromised erythrocyte oxygenation due to poor gas exchange in the lungs (e.g. chronic obstructive pulmonary disease (55), cystic fibrosis (56), asthma (57), and chronic cardiac insufficiency (58)) have been frequently reported to have erythrocytes with atypical deformabilities and/or morphologies. While other explanations are again possible, it should not be ignored that deoxyHb displacement of ankyrin from band 3 might contribute to these structural abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Deoxyhemoglobin with the Cytoplasmic Domain of Murine Erythrocyte Band 3

et al. 2012

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The partial pressure of oxygen constitutes an important factor in the regulation of human erythrocyte physiology, including control of cell volume, membrane structure, and glucose metabolism. Because band 3 is thought to be involved in all three processes and because binding of hemoglobin (Hb) to the cytoplasmic domain of band 3 (cdb3) is strongly oxygen-dependent, the possibility that the reversible association of deoxyhemoglobin (deoxyHb) with cdb3 might constitute an O 2 -dependent sensor that mediates O 2 -regulated changes in erythrocyte properties arises. While several lines of evidence support this hypothesis, a major opposing argument lies in the fact that the deoxyHb binding sequence on human cdb3 is not conserved. Moreover, no effect of O 2 pressure on Hb− band 3 interactions has ever been demonstrated in another species. To explore whether band 3−Hb interactions might be widely involved in O 2 -dependent regulation of erythrocyte physiology, we undertook characterization of the effect of O 2 on band 3−Hb interactions in the mouse. We report here that murine band 3 binds deoxyHb with significantly greater affinity than oxyHb, despite the lack of significant homology within the deoxyHb binding sequence. We further map the deoxyHb binding site on murine band 3 and show that deletion of the site eliminates deoxyHb binding. Finally, we identify mutations in murine cdb3 that either enhance or eliminate its affinity for murine deoxyHb. These data demonstrate that despite a lack of homology in the sequences of both murine band 3 and murine Hb, a strong oxygen-dependent association of the two proteins has been conserved.

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Section: Discussionmentioning

confidence: 99%

Interaction of Deoxyhemoglobin with the Cytoplasmic Domain of Murine Erythrocyte Band 3

et al. 2012

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“…Published data on the effect of exercise on RBC deformability are inconsistent and explained by variation in exercise intensity and volume, study population tested and/or deformability measuring devices applied (Connes et al, 2013). More recent data suggest a beneficial effect of both, acute exercise and chronic training on RBC deformability in healthy and diseased participants (Gürcan et al, 1998; Smith et al, 1999; Suhr et al, 2012; Ahmad et al, 2013, 2014; Koliamitra et al, 2017; Tomschi et al, 2018a). The data presented herein are thus in line with the cited literature indicating that even moderate training might positively affect RBC function.…”

Section: Discussionmentioning

confidence: 99%

Comparisons of Blood Parameters, Red Blood Cell Deformability and Circulating Nitric Oxide Between Males and Females Considering Hormonal Contraception: A Longitudinal Gender Study

et al. 2018

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Red blood cell (RBC) deformability is an important determinant of the microcirculation. It is influenced by various hematological parameters but also by nitric oxide (NO) which is produced in RBC from L-arginine by RBC-NO synthase. Longitudinal studies on blood profile, deformability at rest and NO levels but also differences between males and females (±hormonal contraception; HC) are less known so far. The study thus aimed to investigate RBC deformability, RBC NO species (nitrite, RxNO), RBC L-arginine concentration and basal blood parameters in males and females (±HC) as a function of time. RBC deformability was measured at rest once per week and the remaining parameters were measured once per month, respectively. A second experiment aimed to daily measure RBC deformability and 17β-estradiol in Female ± HC during a whole menstruation cycle to investigate a possible relation of the two parameters. Measured parameters showed low week-to-week variation and remained constant during study period. However, RBC deformability increased in Female + HC during study period possibly because of increasing training volume of the participants. Overall, results indicate gender differences in hematological parameters with higher RBC parameters (RBC count, hematocrit, hemoglobin concentration) in males compared to females. Differences were also observed between the female groups with Females - HC showing lower number of RBC but higher MCV and hematocrit compared to Females + HC. RBC deformability was highest in Females - HC which might be related to permanent higher estradiol levels and/or higher RBC NO levels because RBC nitrite and RBC RxNO concentrations were also highest in Females-HC. Results of the second experiment also suggest higher RBC deformability in Female - HC because of higher estradiol concentrations. L-arginine levels known to be related to RBC NO production were comparable in all groups. In conclusion, hematological, hemorheological and NO related parameters show gender differences. In particular, RBC deformability is affected by training volume and RBC estradiol concentrations. The results add new information on the complex regulation of RBC function which might help to better understand the role of RBC in the microcirculation.

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“…Rheological blood properties were demonstrated to progressively deteriorate as pulmonary insufficiency increases in patients with chronic bronchitis [ 18 ]. There are limited number of studies exploring hemorheological alterations in COPD [ 16 , 20 – 22 ]. The results of these studies were obtained either from COPD patients at different stages of the illness and age [ 18 , 20 , 22 ] or from patients receiving different types of therapy (medication, exercise) [ 16 , 20 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…There are limited number of studies exploring hemorheological alterations in COPD [ 16 , 20 – 22 ]. The results of these studies were obtained either from COPD patients at different stages of the illness and age [ 18 , 20 , 22 ] or from patients receiving different types of therapy (medication, exercise) [ 16 , 20 , 23 ]. Additionally, different types of measurement techniques, some being rather old, were used in a variety of the studies mentioned above, which makes it difficult to compare the results [ 20 , 21 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Impaired Hemorheology in Exacerbations of COPD

Uğurlu

Kilic‐Toprak

Can

et al. 2017

Canadian Respiratory Journal

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Background Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation. Cardiovascular-related comorbidities are established to contribute to morbidity and mortality especially during exacerbations. The aim of the current study was to determine alterations in hemorheology (erythrocyte aggregation, deformability) in newly diagnosed COPD patients and their response to medical treatment and to compare with values of COPD patients with exacerbations. Materials and Methods The study comprised 13 COPD patients, 12 controls, and 16 COPD patients with exacerbations. The severity of COPD was determined according to Global Initiative for Chronic Obstructive Lung Disease guidelines. Red blood cell (RBC) deformability and aggregation were measured by an ektacytometer. Results RBC deformability of COPD patients with exacerbations was decreased compared to the other groups. Erythrocyte aggregation and plasma fibrinogen of COPD patients determined during exacerbations were higher than control. Conclusion Decreased RBC deformability and increased aggregation associated with exacerbations of COPD may serve as unfavorable mechanisms to worsen oxygenation and thus clinical symptoms of the patient. Treatment modalities that modify rheological parameters might be beneficial.

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Influence of a moderate physical activity intervention on red cell deformability in patients suffering from chronic obstructive pulmonary disease (COPD)

Cited by 5 publications

References 52 publications

Interaction of Deoxyhemoglobin with the Cytoplasmic Domain of Murine Erythrocyte Band 3

Interaction of Deoxyhemoglobin with the Cytoplasmic Domain of Murine Erythrocyte Band 3

Comparisons of Blood Parameters, Red Blood Cell Deformability and Circulating Nitric Oxide Between Males and Females Considering Hormonal Contraception: A Longitudinal Gender Study

Impaired Hemorheology in Exacerbations of COPD

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