Influence of 2′-fucosyllactose and galacto-oligosaccharides on the growth and adhesion of <i>Streptococcus mutans</i>

Salli, Krista; Söderling, Eva; Hirvonen, Johanna; Gürsoy, Ulvi Kahraman; Ouwehand, Arthur C.

doi:10.1017/s0007114520001956

Cited by 16 publications

(16 citation statements)

References 51 publications

(87 reference statements)

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“…Previous studies indicated that 2′-FL had growth-inhibiting and adhesion-reducing effects on certain microorganisms [ 25 , 26 ], and thereby the tolerance of B. subtilis to 2′-FL needs to be examined. B. subtilis was cultured in LB medium containing different concentrations of 2′-FL with 96-well plates.…”

Section: Resultsmentioning

confidence: 99%

Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose

Zhang

Liu²,

Xia³

et al. 2022

Microb Cell Fact

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Background The most abundant human milk oligosaccharide in breast milk, 2′-fucosyllactose (2′-FL), has been approved as an additive to infant formula due to its multifarious nutraceutical and pharmaceutical functions in promoting neonate health. However, the low efficiency of de novo synthesis limits the cost-efficient bioproduction of 2′-FL. Results This study achieved 2′-FL de novo synthesis in a generally recognized as safe (GRAS) strain Bacillus subtilis. First, a de novo biosynthetic pathway for 2′-FL was introduced by expressing the manB, manC, gmd, wcaG, and futC genes from Escherichia coli and Helicobacter pylori in B. subtilis, resulting in 2′-FL production of 1.12 g/L. Subsequently, a 2′-FL titer of 2.57 g/L was obtained by reducing the competitive lactose consumption, increasing the regeneration of the cofactor guanosine-5′-triphosphate (GTP), and enhancing the supply of the precursor mannose-6-phosphate (M6P). By replacing the native promoter of endogenous manA gene (encoding M6P isomerase) with a constitutive promoter P7, the 2′-FL titer in shake flask reached 18.27 g/L. The finally engineered strain BS21 could produce 88.3 g/L 2′-FL with a yield of 0.61 g/g lactose in a 3-L bioreactor, without the addition of antibiotics and chemical inducers. Conclusions The efficient de novo synthesis of 2′-FL can be achieved by the engineered B. subtilis, paving the way for the large-scale bioproduction of 2′-FL titer in the future.

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Section: Resultsmentioning

confidence: 99%

Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose

Zhang

Liu²,

Xia³

et al. 2022

Microb Cell Fact

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“…Infant oral microbiota are important for oral health [ 79 ] and differences in the oral microbiota between breastfed and formula-fed infants have been reported [ 91 , 95 , 96 ], however, little is known about how HMOs impact the oral microbiota. A recent in vitro study assessed the effect of 2′FL and galacto-oligosaccharides on the growth and adhesion characteristics of the caries-associated oral pathogen Streptococcus mutans and reported 2′FL both limits growth and inhibits adhesion of S. mutans to saliva-coated hydroxyapatite [ 97 ]. However, there is an absence of information on how daily intakes of HMOs influence infant oral microbiota.…”

Section: Discussionmentioning

confidence: 99%

Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes

Cheema

Trevenen

Turlach

et al. 2022

IJMS

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Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health. This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles. Maternal and infant samples (n = 10) were collected at 2–5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced. Nineteen HMOs were quantitated using high-performance liquid chromatography. Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types. Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2–5 significantly differed from all other time points (all p < 0.02). HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples. The direct clinical relevance of this, however, is unknown. Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.

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“…Another study on the growth and adhesion of Streptococcus mutans found that GOS promoted the growth of Streptococcus mutans as a carbon source, while 2′-FL could not. Both could reduce the adhesion of pathogenic bacteria, but the mode of inhibition might be different [ 30 ]. Previous studies in our laboratory confirmed that 2′-FL intervention could regulate the composition of the gut microbiota, and the colonization of E. coli O157 in the intestines of mice was decreased by over 90%.…”

Section: Introductionmentioning

confidence: 99%

2′-Fucosyllactose Ameliorates Oxidative Stress Damage in d-Galactose-Induced Aging Mice by Regulating Gut Microbiota and AMPK/SIRT1/FOXO1 Pathway

Wang

Song

et al. 2022

Foods

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The imbalance of reactive oxygen species is the main cause in aging, accompanied by oxidative stress. As the most abundant in human milk oligosaccharides (HMOs), 2′-Fucosyllactose (2′-FL) has been confirmed to have great properties in immunity regulation and anti-inflammatory. The research on 2′-FL is focused on infants currently, while there is no related report of 2′-FL for the elderly. A d-galactose-induced accelerated aging model was established to explore the protective effect of 2′-FL on the intestines and brain in mice. In this study, 2′-FL significantly reduced oxidative stress damage and inflammation in the intestines of aging mice, potentially by regulating the sirtuin1 (SIRT1)-related and nuclear factor E2-related factor 2 (Nrf2) pathways. In addition, 2′-FL significantly improved the gut mucosal barrier function and increased the content of short-chain fatty acids (SCFAs) in the intestine. The gut microbiota analysis indicated that 2′-FL mainly increased the abundance of probiotics like Akkermansia in aging mice. Moreover, 2′-FL significantly inhibited apoptosis in the brains of aging mice, also increasing the expression of SIRT1. These findings provided a basis for learning the benefits of 2′-FL in the aging process.

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Influence of 2′-fucosyllactose and galacto-oligosaccharides on the growth and adhesion of Streptococcus mutans

Cited by 16 publications

References 51 publications

Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose

Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose

Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes

2′-Fucosyllactose Ameliorates Oxidative Stress Damage in d-Galactose-Induced Aging Mice by Regulating Gut Microbiota and AMPK/SIRT1/FOXO1 Pathway

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