Influence mechanism of miRNA‑144 on proliferation and apoptosis of osteosarcoma cells

Abstract

Influence mechanism of miRNA-144 on proliferation and apoptosis of osteosarcoma cells was investigated. A total of 51 cases of osteosarcoma tissue samples were collected in the department of orthopedic surgery, Xuzhou Children's Hospital, Xuzhou Medical University from January 2014 to February 2017. Additionally, 48 cases of normal bone tissues were collected. qRT-PCR was used to detect the expression of miR-144. Correlation of miR-144 expression in serum and cancer tissues was detected. ROC curve was drawn to analyze the diagnostic value of miR-144 in patients with osteosarcoma. CCK-8 was used to detect the effect of miR-144 on the proliferation ability of U2-OS after transfection. The ratio of U2-OS apoptosis after miR-144 transfection was detected by flow cytometry. Western blot analysis was used to detect the expression of Bax, caspase-3 and Bcl-2 proteins in U2-OS after transfection. The relative expression of miR-144 in osteosarcoma and osteosarcoma serum was significantly lower than that in normal bone tissue and normal human serum (P<0.05). Serum in patients was positively correlated with the expression of miR-144 in cancer tissues. The area under the miR-144 curve was 0.852, 95% CI, 0.768-0.936. The relative expression of miR-144 in MG-63 and U2-OS cells was significantly lower than that in hFOB1.19 cells (P<0.05), while significantly lower in U2-OS cells than in MG-63 cells (P<0.05). Proliferation ability of U2-OS cells transfected with miR-144-mimics was significantly inhibited and the apoptosis rate was significantly increased (P<0.05). Bcl-2 protein was significantly decreased by detection of WB and the expression of Bax and caspase-3 protein was significantly increased (P<0.05). miR-144 may be involved in the occurrence and deterioration of osteosarcoma. miR-144 can regulate proliferation and apoptosis of U2-OS cells. It is expected to become a new diagnostic and index target for osteosarcoma.

Identification of potential micro-messenger RNAs (miRNA–mRNA) interaction network of osteosarcoma

“…MicroRNAs (miRNAs) are a class of non-coding small RNA about 20-24 nucleotides [6,7]. By targeting mRNA, miRNAs could regulate the process of biological processes, cell proliferation, cell division, apoptosis, cell invasion, metastasis, metabolism, and tumorigenesis [1,8,9].…”

Non-coding RNA-related antitumor mechanisms of marine-derived agents

“…Meanwhile, it was also found that AX2 decreased the expression level of miRNA144, and the direct target gene of miR-144 was subsequently identified as mTOR ( Liu et al, 2016 ). However, miR-144 acts as a tumor suppressor gene to regulate cell proliferation, apoptosis and autophagy ( Xiao et al, 2015 ; Wang et al, 2017c ; Zhang et al, 2020b ). In addition, it is well known that mTOR is a positive feedback regulator of the PI3K/Akt/mTOR pathway.…”