Inflammatory response and immunohistochemical characterization of experimental calcium silicate‐based perforation repair material

Abstract

This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided into three experimental groups (n = 54 teeth each) according to the evaluation period: 1, 2 and 3 months. Each group was further divided into two equal subgroups (n = 27 teeth each) according to the time of repair: immediate repair and delayed repair. Each subgroup was subdivided according to the material used into three experimental subdivisions (n = 8 teeth each): MTA, Biodentine (Septodont) and experimental material, and two control subdivisions: positive control (n = 2 teeth) and negative control (one tooth). Under general anaesthesia, access cavity How to cite this article: Okasha H, Abu-Seida AM, Hashem AA, El Ashry SH, Nagy MM. Inflammatory response and immunohistochemical characterization of experimental calcium silicatebased perforation repair material. Int

Histopathological and immunohistochemical profiles of pulp tissues in immature dogs’ teeth to two recently introduced pulpotomy materials

“…Osteopontin (OPN) is an acidic glycoprotein found in the extracellular matrix that has been linked to a variety of physiological and pathological processes [ 36 ]. Many studies deemed OPN a mineralization marker therefore it was used as the immunohistochemistry marker in our 90-day assessment [ 37 , 38 ] Furthermore, the elevated expression of OPN in reparative dentin was thought to be a sign of the existence of odontoblast cells by Tziafas et al [ 39 ] The results revealed that MTA subgroups have moderate to a strong representation of OPN intervening the hard tissue bridge, which is directly linked to MTA's ability to recruit cells such as fibroblasts and osteoblast-like cells, which are the main stimulants to the expression of genes of hard tissue–related proteins (OPN) [ 40 ]. Similarly, at 60 and 90 days, Cosme-Silva et al [ 21 ] noticed that the white MTA-Angelus groups had moderate OPN marker immunolabeling.…”

Quantitative assessment of brown adipose tissue whitening in a high-fat-diet murine model using synthetic magnetic resonance imaging

“…UCP1 quantitative analysis was assessed using the means of the immunopositive area fraction of UCP1 using ImageJ software (version 1.8.0). In the photomicrographs, the immunopositive area fraction was automatically measured as the positive immunoreaction, which was defined by the immunopositive area as a percentage of the total area of the microscopic field [ 21 , 22 ]. The mean immunopositive area fraction for each case was calculated.…”

Modulating proliferative and osteogenic potentials of mesenchymal stem cells via a novel formulation of MTA as a root perforation repair material

“…The site and size of the perforation, the time delay before treatment, the presence of microorganism contamination, the osteogenic differentiation potential, the sealing ability of the restorative material, and the tooth's periodontal condition all in uence the long-term prognosis of this complication 7 . A material with outstanding physiochemical, mechanical, and biocompatibility qualities is well recommended 8,9 .…”

“…Most clinicians recommend MTA for pulp capping, root perforation repair, retrograde lling restoration, apexi cation, revascularization, resorption repair, and apical surgery because of its sealing ability, marginal adaptation, and biocompatibility as determined by cytotoxicity and genotoxicity tests of cell cultures or in vivo studies 2,3,6,7 . The disadvantages of MTA include a long initial setting time (3-4 hours), di culty handling (depending on the water/powder ratio), high cost, restricted radiopacity, discoloration when in contact with sodium hypochlorite, and wash-out in humid settings 12,13 .…”

“…A number of materials, including amalgam, Cavit, IRM, Super-EBA, glass ionomer cements, and resins, have been used for this purpose. Mineral trioxide aggregate (MTA) and other calcium-silicate cements have been successfully used for perforation repair because they create a regenerative environment and stimulate new bone formation through osteoinductive action 3,6,7,11 .…”