Histopathological and immunohistochemical profiles of pulp tissues in immature dogs’ teeth to two recently introduced pulpotomy materials

Abstract

Objective The pulpal response to Hoffmann’s Pulpine mineral (PMIN) and Pulpine NE (PNE) was compared to mineral trioxide aggregate (MTA) when used as pulpotomy materials in immature permanent teeth in dogs. Materials and methods Immature premolars were randomly divided according to the observation period into three equal groups (n = 24) (10 days, 30 days, and 90 days) then furtherly subdivided into 3 subgroups according to the material used. Histopathological analysis regarding inflammatory cell infiltration and dentin bridge (DB) formation was done. Immunohistochemical analysis was performed using osteopontin marker. Results The results showed that after 90 days, both MTA and PMIN subgroups had 100% complete thick DB without inflammation in 87.5% of the samples, while the PNE subgroup failed to form DB in 37.5% of the samples and 50% of samples showed thin initial DB with heavy inflammation in 62.5% of the samples. There was no significant difference between MTA and PMIN, while there was a statistically significant difference between PNE and the two other subgroups in DB formation and inflammatory cell infiltration (P > 0.05). After 90 days, MTA showed the highest mean value of osteopontin positive fraction area followed by PMIN without statistically significant differences, while the least value was recorded in PNE subgroup with statistically significant difference with the remaining subgroups (P < 0.05). Conclusion PMIN is a promising alternative to MTA when used for pulpotomy. Clinical relevance Vital pulp therapy in immature teeth can be done using PMIN as an alternative to MTA.

Physicochemical characteristics and discolouration potentials of Pulpine mineral® and Pulpine NE®

“…The results of pH values are collected in table (3). At 24 hours and one week, the greatest pH value was recorded in PMIN group , followed by MTA group, while the least value was recorded in PNE group with no statistically significant difference between the MTA and PMIN groups, but a statistically significant difference was found between the PNE group and other groups (P<0.05).…”

“…Because of its outstanding biocompatibility and capacity to build dentin bridge with minimal inflammation, MTA is the gold standard material applied for VPT [2]. Unfortunately, it showed poor handling characteristics, long setting time and crown discoloration [3].…”

Cytotoxic Effect of Tideglusib on Human Fibroblasts: An In Vitro Study

“…Absorbance of each well at a wavelength of 570 nm using an ELISA plate reader spectrophotometer (ELX-800, Biotek, USA) was recorded. 24 For Tideglusib and positive control groups, the following formula determined the cell viability percentage [Mean OD of treatment group/Mean OD of negative control group] × 100% 1,2 . Cytotoxicity responses were scored as: severe cytotoxic ≤30%, moderate cytotoxic 30%-60%, mild cytotoxic 60%-90% and non-cytotoxic ≥90%.…”

“…Microorganisms can penetrate the root canal system quickly through exposed dentinal tubules in the presence of necrotic pulp owing to lack of pulp defensive function. 1,2 Odontoblasts responsible for pulp protection by secretion of extracellular dentin matrix which protect the pulp against external stimuli. 3 However, teeth with vital pulps demonstrate greater resistance to bacterial invasion.…”

Potential Benefits of Propolis in Large and Small Animal Practices: A Narrative Review of the Literature

“…Propolis is commonly applied in veterinary medicine for its antibacterial (El-Tayeb et al, 2019;Przybyłek andKarpiński, 2019), antifungal (de Mendonça et al, 2015), antiviral (Alp, 2018), antiparasitic (Anjum et al, 2019), antioxidant (Torres et al, 2018), anti-inflammatory (Machado et al, 2016), antitumor (Doğan et al, 2020), immunomodulatory (Shvarzbeyn andHuleihel, 2011), antiulcer (da Silva et al, 2018), local anesthetic (Özdemir et al, 2022), and cytotoxic (Bonamigo et al, 2017) actions as shown in Figure 2. Moreover, propolis enhances wound healing (Abu-Seida, 2015), dentinogenesis after pulp capping (Saleh et al, 2016;Abo El-Mal et al, 2021), and hard-tissue deposition as well as soft-tissue formation inside the necrotic dental pulp in dogs (El-Tayeb et al, 2019;Abdelsalam et al, 2020;Abo EL Wafa et al, 2021;Mohamed et al, 2023). Machado et al, 2016;Torres et al, 2018;Anjum et al, 2019;Doğan et al, 2020;Özdemir et al, 2022).…”