Infeksi Virus Dengue pada Nyamuk Aedes aegypti Menggunakan Artificial Blood Feeding dan Deteksi Virus Dengue Menggunakan Teknik Molekular

Abstract

Abstract. Artificial blood-feeding using the parafilm-M membrane can be used as an alternative solution andsubstitute live animals as a source of blood. This method is not only be used for blood-feeding but also to infectthe dengue virus (DENV) to mosquitoes. This study was aimed to determine the effectiveness artificial bloodfeeding using parafilm-M membrane in Aedes mosquitoes originated in Indonesia and determine the positivityof mosquitoes infected by Indonesia DENV-1. DENV-1 was isolated from patient and propagated in Vero cellculture. The feeding was done in cardboard cups after mosquitos have been starved for 4-17 hours before beingfed with human blood. A conical 50ml tube was prepared, and a hole was created in the tube lid. The tubeopening was covered with parafilm. Glycerol was added into conical tube and heated in water bath for an hourat 55oC. A mixture of blood and DENV-1 was made with concentration of 10%. Detection of DENV in bloodfedmosquitos was carried out by using Simplexa Dengue Real-Time RT-PCR assay. The results showed thatthe prevalence of blood-fed mosquitoes reached 66.67% with fasting period for 17 hours. Blood feedingmosquitoes are affected by duration of fasting period, blood-feeding time, and attractants from human skinrubbed into parafilm-M membrane. The prevalence of blood-fed Ae. aegypti infected by DENV was 20.83%.This study provides information on the effectiveness of artificial parafilm membrane blood-feeding in alaboratory setting that will be useful for vector control study in Indonesia.

Aktivitas Ekstrak Daun Tanaman Bunga Terompet Emas (Allamanda cathartica L.) terhadap Mortalitas Larva Aedes Aegypti

“…Beberapa kasus yang parah dapat menyebabkan perdarahan internal, adanya trombositopenia dan hemokonsentrasi (Masihor et al, 2013). Disisi lain, nyamuk Aedes aegypti sebagai vektor primer pembawa virus dengue memiliki sifat multiple feeding, keadaan tersebut dapat memperparah penyebaran DBD (Kusmintarsih et al, 2019), karena satu nyamuk yang terinfeksi virus dengue dapat menularkan virus tersebut kepada lebih dari satu orang dalam satu kali makan (Carrington & Simmons, 2014). Pencegahan penyakit DBD saat ini masih berfokus pada pengendalian vektor dengan melibatkan masyarakat secara aktif melalui gerakan nasional seperti penggunaan insektisida dan larvasida, fogging fokus, kelambu dan 3M, jumantik, PSN, COMBI, dan juga program G1R1J (Kemkes RI, 2021).…”

Eliminasi Endosimbion Wolbachia sp. pada Nyamuk Aedes albopictus dengan Antibiotik Tetrasiklin

“…Hal ini karena pemberian darah frekuensinya hanya 4 hari sekali, kemungkinan tidak cukup untuk segera mematangkan dan menghasilkan telur. Hasil penelitian sesuai dengan penelitian sebelumnya 30,31,32,33 yang menyatakan bahwa frekuensi penghisapan darah berpengaruh terhadap fertilitas telur. Nyamuk dewasa yang sudah bertelur pada generasi F1, F2, dan F3 dianalisis dengan uji PCR untuk mengetahui tereliminasinya endosimbion Wolbachia.…”