Infecção por leptospiras em ovinos e caracterização da resposta inflamatória renal

Abstract

RESUMO.-As Leptospiroses são zoonoses graves de distribuição mundial que afetam o homem e outros animais. A infecção em animais, geralmente, é inaparente, ou os sintomas quando presentes são similares a outras infecções. Neste estudo foram colhidos soros de 119 ovinos e seus respectivos rins durante abate em feiras livres no município de Teresina-Piauí. Pela técnica de soroaglutinação microscópica (SAM) obtiveram-se 34 amostras sorológicas positivas para um ou mais sorovares de Leptospira spp., com taxa de ocorrência de 28,6% de anticorpos anti-leptospiras, sendo 23 casos de infecção para um único sorovar e 11 com coaglutinações para dois ou mais sorovares. Lepitospirosis is a serious worldwide distribution disease which affects man and other animals. The infection is generally asymptomatic in animals. In cases whose symptoms are present, symptoms are similar to other infections. In the present study serum samples from 119 sheep and their kidneys were collected during their slaughter in outdoor markets in the city of Teresina, Piauí, Brazil. The Microscopic Agglutination Test (MAT) obtained 34 positive serological samples for one or more Leptospira spp. serovar with occurrence rate of 28.6% of leptospiral antibodies. There were 23 cases of infection for a single serovar, and 11 cases with coagglutination for two or more serovars. Autumnalis had the highest occurrence (29.4%) among the pathogenic serovars. The histopathological analysis of 36 kidney fragments revealed tubulo-interstitial alterations in 33 (91.7%) positive animals. Tubular lesions were observed in 20 (55.5%) positive animals. The Warthin Starry staning revealed the presence of Leptospira in 8 (22.20%) of the 36 positive samples. The immunoperoxidase staining revealed the presence of Leptospira in 12 (60%) of 20 positive samples. The inflammatory infiltrate in the positive animals was significantly more evident in the cortical-medullar and cortical regions than in the medullar region (p=0.000), however, there was no difference between positive and negative animals. The presence of hyaline casts in the proximal tubules was significantly higher in positive animals compared to the negative ones (p=0.0001). Discrete lesion was observed in glomeruli. In conclusion, the results from this study showed that sheep which are positive for Leptospira present tubulo-intersticial renal lesions with the presence of Leptospira in the tubules, conferring to these animals the condition of asymptomatic carriers.

Influence of storage on the chemical composition of Stylosanthes hays for equine feeding

“…Hardjobovis and Autumnalis were the most frequent serovars of sheep and goats found in this study, a result that corroborates the findings of recent serological surveys conducted on small ruminants in Brazil (HERRMANN et al, 2004;LILENBAUM et al, 2007;ARAÚJO NETO et al, 2010;HIGINO et al, 2010;SALABERRY et al, 2011;CARVALHO et al, 2011;SANTOS et al, 2012;MARTINS et al, 2012;MORAES et al, 2012;ALVES et al, 2012;HIGINO et al, 2012;RIZZO et al, 2014). It was long believed that these animals acquired leptospirosis only by prior contact with other species (LEON-VIZCAINO et al, 1987), although the breeding of these species together with cattle breeding and the presence of rodents are important factors for the dissemination of the Hardjobovis and Autumnalis serovars, respectively (FAINE et al, 1999;SALABERRY et al, 2011;SEIXAS et al, 2011).…”

Epidemiologic analysis of Leptospira spp. infection among sheep in Pernambuco state, Brazil

“…The serovar Autumnalis, found in 32.4% of the animals, has been identified mainly in the Brazilian northeast (Borba, 2004;Higino et al, 2010;Carvalho et al, 2011;Adams et al, 2012). In 2007, Leptospira nouguchii species was isolated in apparently healthy sheep, showing that these animals can act as hosts for the serovar Autumnalis (Silva et al, 2007).…”

“…Studies conducted by different microscopic serum agglutination techniques in some States of Brazil have detected anti-Leptospira antibody in sheep, with wide variation of anti-Leptospira prevalence and serovars (Langoni et al, 1995;Caldas et al, 1995;1996;Mota et al, 1999;Herrmann et al, 2004;Aguiar et al, 2010;Hashimoto et al, 2010;Higino et al, 2010, Carvalho et al, 2011Salaberry et al, 2011;Alves et al, 2012;Martins et al, 2012).…”

Inquérito sorológico para leptospirose em rebanhos de ovinos no município de Igarapé-Açu, Estado do Pará

“…No presente trabalho, houve a predominância de animais reagentes para o sorovar Autumnalis (9,62%), à semelhança do observado por Carvalho et al (2011) em ovinos no Estado do Piauí. Contudo, esse sorovar ainda não foi isolado no Brasil e, portanto, novas investigações com técnicas de isolamento e tipificação de estirpes deverão ser realizadas para confirmação da presença deste em rebanhos de ovinos da região.…”