Inertial migration of cancer cells in blood flow in microchannels

Tanaka, Tatsuya; Ishikawa, Takuji; Numayama-Tsuruta, Keiko; Imai, Yohsuke; Ueno, Hironori; Yoshimoto, Takefumi; Matsuki, Noriaki; Yamaguchi, Takami

doi:10.1007/s10544-011-9582-y

Cited by 49 publications

(45 citation statements)

References 18 publications

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“…After the first observation of inertial migration, a number of experimental studies and theoretical analyses were conducted to explore the underlying mechanism of this phenomenon [14][15][16][17][18][19][20][21] . This phenomenon had not found its practical application until the emergence of microfluidic technology, where particle size is comparable with characteristic dimension of microchannel.…”

Section: Introductionmentioning

confidence: 99%

Fundamentals and applications of inertial microfluidics: a review

et al. 2016

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In the last decade, inertial microfluidics has attracted significant attention and a wide variety of channel designs that focus, concentrate and separate particles and fluids have been demonstrated. In contrast to conventional microfluidic technologies, where fluid inertia is negligible and flow remains almost within the Stokes flow region with very low Reynolds number (Re < 1), inertial microfluidics works in the intermediate Reynolds number range (~1 < Re < ~100) between Stokes and turbulent regimes. In this intermediate range, both inertia and fluid viscosity are finite and bring about several intriguing effects that form the basis of inertial microfluidics including (i) inertial migration and (ii) secondary flow. Due to the superior features of high-throughput, simplicity, precise manipulation and low cost, inertial microfluidics is a very promising candidate for cellular sample processing, especially for samples with low abundant targets. In this review, we first discuss the fundamental kinematics of particles in microchannels to familiarise readers with the mechanisms and underlying physics in inertial microfluidic systems. We then present a comprehensive review of recent developments and key applications of inertial microfluidic systems according to their microchannel structures. Finally, we discuss the perspective of employing fluid inertia in microfluidics for particle manipulation. Due to the superior benefits of inertial microfluidics, this promising technology will still be an attractive topic in the near future, with more novel designs and further applications in biology, medicine and industry on the horizon. ABSTRACT:In the last decade, inertial microfluidics has attracted significant attention and a wide variety of channel designs that focus, concentrate and separate particles and fluids have been demonstrated. In contrast to conventional microfluidic technologies, where fluid inertia is negligible and flow remains almost within Stokes flow region with very low Reynolds number Re <<1, inertial microfluidics works in the intermediate Reynolds number range (~1 < Re < ~100) between Stokes and turbulent regimes. In this intermediate range, both inertia and fluid viscosity are finite, and bring about several intriguing effects that form the basis of inertial microfluidics including: (i) inertial migration and (ii) secondary flow. Due to the superior features of high-throughput, simplicity, precise manipulation and low cost, inertial microfluidics is a very promising candidate for cellular sample processing, especially for sample with low abundant targets. In this review, we first discuss the fundamental kinematics of particles in microchannels to familiarise readers with mechanisms and underlying physics in inertial microfluidic systems. We then present a comprehensive review of recent developments and key applications of inertial microfluidic systems according to their microchannel structures. Finally, we discuss the perspective of employing fluid inertia in microfluidics for particle manipu...

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Section: Introductionmentioning

confidence: 99%

Fundamentals and applications of inertial microfluidics: a review

et al. 2016

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“…14,18,112,141 When a particle flows parallel to a wall with a non-negligible inertia effect, the particle experiences an inertia-induced lift force perpendicular to the wall. Asmolov et al 10 calculated the inertia-induced lift force F L of a rigid sphere flowing in a straight circular tube by asymptotic analysis.…”

Section: Microfluidic Devices For Blood-cell Separationmentioning

confidence: 99%

“…The effects of cancer cell-RBC interactions on the inertial migration forces were investigated by Tanaka et al 141 using a non-dilute suspension of RBCs. The volume fraction of RBCs, i.e., hematocrit (Hct), used in the study varied from 0% to a concentrated regime (40%) where the cell-cell interactions significantly affected the cells' migration behavior.…”

Section: Microfluidic Devices For Separation Of Cancer Cells From Bloodmentioning

confidence: 99%

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Hemodynamics in the Microcirculation and in Microfluidics

et al. 2014

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Hemodynamics in microcirculation is important for hemorheology and several types of circulatory disease. Although hemodynamics research has a long history, the field continues to expand due to recent advancements in numerical and experimental techniques at the micro-and nano-scales. In this paper, we review recent computational and experimental studies of blood flow in microcirculation and microfluidics. We first focus on the computational studies of red blood cell (RBC) dynamics, from the single cellular level to mesoscopic multiple cellular flows, followed by a review of recent computational adhesion models for white blood cells, platelets, and malaria-infected RBCs, in which the cell adhesion to the vascular wall is essential for cellular function. Recent developments in optical microscopy have enabled the observation of flowing blood cells in microfluidics. Experimental particle image velocimetry and particle tracking velocimetry techniques are described in this article. Advancements in micro total analysis system technologies have facilitated flowing cell separation with microfluidic devices, which can be used for biomedical applications, such as a diagnostic tool for breast cancer or large intestinal tumors. In this paper, cell-separation techniques are reviewed for microfluidic devices, emphasizing recent advances and the potential of this fast-evolving research field in the near future.

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“…Tanaka et al 63,64 studied the separation of CTCs from RBCs using inertial migration of cells in a microfluidic device. The device was designed based on the observation that the cancer cells migrated to an equilibrium position of 0.6 Â 1 = 2 channel-width measured from the center of cells to the center of the channel.…”

Section: Microfluidic Separation Based On Inertial Forces (Figures 1(mentioning

confidence: 99%

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

et al. 2013

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This review will cover the recent advances in label-free approaches to isolate and manipulate circulating tumor cells (CTCs). In essence, label-free approaches do not rely on antibodies or biological markers for labeling the cells of interest, but enrich them using the differential physical properties intrinsic to cancer and blood cells. We will discuss technologies that isolate cells based on their biomechanical and electrical properties. Label-free approaches to analyze CTCs have been recently invoked as a valid alternative to "marker-based" techniques, because classical epithelial and tumor markers are lost on some CTC populations and there is no comprehensive phenotypic definition for CTCs. We will highlight the advantages and drawbacks of these technologies and the status on their implementation in the clinics.

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Inertial migration of cancer cells in blood flow in microchannels

Cited by 49 publications

References 18 publications

Fundamentals and applications of inertial microfluidics: a review

Fundamentals and applications of inertial microfluidics: a review

Hemodynamics in the Microcirculation and in Microfluidics

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

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