Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

Cima, Igor; Yee, Chay Wen; Iliescu, Florina Silvia; Phyo, Wai Min; Lim, Kiat Hon; Iliescu, Ciprian; Tan, Min

doi:10.1063/1.4780062

Cited by 98 publications

(74 citation statements)

References 148 publications

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“…[8][9][10] Therefore, a fundamental understanding of the detailed fluid dynamics of blood flow and of the distribution of the wall shear stress in small vessels is essential to help detect cardiovascular diseases and to develop preventive measures and design suitable treatments. 11 Moreover, recent developments in blood sample loading and blood/tumor cell sorting devices [12][13][14] warrant the study of the viscoelastic properties of blood in microfluidic devices. a) campo@fe.up.pt and laura@campodeano.com The manipulation of real whole blood and the study of its flow dynamics in vitro are difficult because of the cost, safety, and ethics issues involved.…”

Section: Introductionmentioning

confidence: 99%

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

et al. 2013

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The non-Newtonian properties of blood are of great importance since they are closely related with incident cardiovascular diseases. A good understanding of the hemodynamics through the main vessels of the human circulatory system is thus fundamental in the detection and especially in the treatment of these diseases. Very often such studies take place in vitro for convenience and better flow control and these generally require blood analogue solutions that not only adequately mimic the viscoelastic properties of blood but also minimize undesirable optical distortions arising from vessel curvature that could interfere in flow visualizations or particle image velocimetry measurements. In this work, we present the viscoelastic moduli of whole human blood obtained by means of passive microrheology experiments. These results and existing shear and extensional rheological data for whole human blood in the literature enabled us to develop solutions with rheological behavior analogous to real whole blood and with a refractive index suited for PDMS (polydymethylsiloxane) micro-and milli-channels. In addition, these blood analogues can be modified in order to obtain a larger range of refractive indices from 1.38 to 1.43 to match the refractive index of several materials other than PDMS. V C 2013 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

et al. 2013

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“…These technologies are typically based around microfluidic [5] or optical [6] technologies (and often in combination), to provide the levels of both precision and throughput required to make such single cell investigations feasible. In particular, the experiments seek to quantify the differences in individual cellular responses to stimuli, be they drugs, oxidative environments or nucleic acids.…”

Section: Introductionmentioning

confidence: 99%

A Novel, All-Optical Tool for Controllable and Non-Destructive Poration of Cells with Single-Micron Resolution

Casey

Wylie

Gallo

et al. 2015

Optics in the Life Sciences

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Abstract:We demonstrate controllable poration within ≈1 µm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience.As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single-and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

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“…Recently, there have been many attempts to develop a reliable, rapid and sensitive method for the isolation and detection of CTCs, including immunomagnetic separation, 2,3 affinity chromatography separation, 4,5 and label-free separation. [6][7][8][9][10][11][12] Despite the detection and analysis of CTCs offer a promising non-invasive diagnostic approach for the diagnosis and prognosis of cancer, its use in clinical practice remains limited because of the rarity of CTCs in blood, the difficulty of isolating CTCs and the complexity of CTCs identification and enumeration. In contrast to the isolation and detection of CTCs, the protein-based biomarker tests are easier and more convenient which do not need labor-intensive cell capture and identification and has the potential to allow rapid point-of-care use.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Zhang

Liao

et al. 2013

Biomicrofluidics

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Cancer biomarkers have significant potential as reliable tools for the early detection of the disease and for monitoring its recurrence. However, most current methods for biomarker detection have technical difficulties (such as sample preparation and specific detector requirements) which limit their application in point of care diagnostics. We developed an extremely simple, power-free microfluidic system for direct detection of cancer biomarkers in microliter volumes of whole blood. CEA and CYFRA21-1 were chosen as model cancer biomarkers. The system automatically extracted blood plasma from less than 3 ll of whole blood and performed a multiplex sample-to-answer assay (nano-ELISA (enzyme-linked immunosorbent assay) technique) without the use of external power or extra components. By taking advantage of the nano-ELISA technique, this microfluidic system detected CEA at a concentration of 50 pg/ml and CYFRA21-1 at a concentration of 60 pg/ml within 60 min. The combination of PnP polydimethylsiloxane (PDMS) pump and nano-ELISA technique in a single microchip system shows great promise for the detection of cancer biomarkers in a drop of blood. V C 2013 AIP Publishing LLC. [http://dx

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Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

Cited by 98 publications

References 148 publications

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

A Novel, All-Optical Tool for Controllable and Non-Destructive Poration of Cells with Single-Micron Resolution

Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

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