Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Zhang, Honglian; Li, Gang; Liao, Lingying; Mao, Hongju; Jin, Qinghui; Zhao, Jianlong

doi:10.1063/1.4807803

Cited by 11 publications

(11 citation statements)

References 45 publications

(35 reference statements)

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“…PDMS slab at the outlet pulls the liquid further into the channel via degassing mechanism. 62 The sedimentation area is a trench like structure which is deeper and wider than other areas to slow down the flow rate and allows blood cells to sediment under gravity. All the reagents including plasma, wash buffers, and detection antibody-gold-silver complex were pumped to the reaction site at different times with the PDMS pump.…”

Section: Cancer Detection At Poc – Research Updatementioning

confidence: 99%

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Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Dixit

Kadimisetty

Otieno

et al. 2016

Analyst

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Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses. Simultaneous detection of panels of biomarker proteins holds great promise as a general tool for reliable cancer diagnostics. A major challenge in designing such a panel is to decide upon a coherent group of biomarkers which have higher specificity for a given type of cancer. The second big challenge is to develop test devices to measure these biomarkers quantitatively with high sensitivity and specificity, such that there are no interferences from the complex serum or tissue matrices. Lastly, integrating all these tests into a technology that doesn’t require exclusive training to operate, and can be used at point-of-care (POC) is another potential bottleneck in futuristic cancer diagnostics. In this article, we review electrochemistry-based tools and technologies developed and/or used in our laboratories to construct low-cost microfluidic protein arrays for highly sensitive detection of the panel of cancer-specific biomarkers with high specificity and at the same time have the potential to be translated into a POC.

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Section: Cancer Detection At Poc – Research Updatementioning

confidence: 99%

“…They have achieved low detection limits in pg/mL range with CEA at a concentration of 50 pg/mL and CYFRA21-1 at a concentration of 60 pg/mL in less than 60 min. 62 …”

Section: Cancer Detection At Poc – Research Updatementioning

confidence: 99%

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Dixit

Kadimisetty

Otieno

et al. 2016

Analyst

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show abstract

“…Microfluidic technology has broad applications in basic research and clinical analysis due to its portability, affordability, and high sensitivity (Nora Dickson et al, 2011;Zhang et al, 2013;Zhao et al, 2013). It holds great promise as it can perform typical laboratory operations using a small volume of reagents in less time (Teh et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Absolute quantification of lung cancer related microRNA by droplet digital PCR

Wang

et al. 2015

Biosensors and Bioelectronics

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“…Passive separation techniques can be stored under pressure or driven by capillary forces [ 13 , 14 , 15 ]. Using capillary forces in plasma separation is more desirable, since it demands neither vacuum packaging, nor degassing of a suction material [ 16 ]. The mechanism of separation is generally based on sedimentation, size exception, or a combination of them [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Microfluidic Device for Blood Plasma Filtration

et al. 2021

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The use of whole blood and some biological specimens, such as urine, saliva, and seminal fluid are limited in clinical laboratory analysis due to the interference of proteins with other small molecules in the matrix and blood cells with optical detection methods. Previously, we developed a microfluidic device featuring an electrokinetic size and mobility trap (SMT) for on-chip extract, concentrate, and separate small molecules from a biological sample like whole blood. The device was used to on-chip filtrate the whole blood from the blood cells and plasma proteins and then on-chip extract and separate the aminoglycoside antibiotic drugs within 3 min. Herein, a novel microfluidic device featuring a nano-junction similar to those reported in the previous work formed by dielectric breakdown was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield of 62% within less than 5 min. The filtered plasma was analyzed using our previous device to show the ability of this new device to remove blood cells and plasma proteins. The filtration device shows a high yield of plasma allowing it to detect a low concentration of analytes from the whole blood.

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Direct detection of cancer biomarkers in blood using a “place n play” modular polydimethylsiloxane pump

Cited by 11 publications

References 45 publications

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics

Absolute quantification of lung cancer related microRNA by droplet digital PCR

A Novel Microfluidic Device for Blood Plasma Filtration

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