Induksi dan Multiplikasi Tunas Alfalfa (Medicago sativa L) secara In Vitro untuk Penyediaan Bibit Tanaman Pakan Ternak

Abstract

Multiplication of alfalfa through in vitro culture is an alternative technique to conventional one to solve the problem on forage without seed production. An experiment was done to obtain the best culture media for shoot induction and multiplication of alfalfa. This research was conducted at the tissue culture in BB Biogen laboratory. The seed of alfalfa was used which was cultured on MS media and seeds were then transferred on seven sterilization treatment BA 0.5 mg/l + GA (0.1; 0.5; and 1.0 mg/l) media germination. Shoot multiplication of alfalfa were eight treatment on growth were MS + BA (0.0; 0.5; and 1.0 mg/l) with combination with thidiazuron (TDZ) (0.0; 3.0; and 5.0 mg/l). Results showed that conducted the highest number of shoot multiplication of alfalfa was obtained on the media containing BA 0.5 mg/l and TDZ 3.0 mg/l (9.6 shoots and shoot length was observed at average 14.6 mm and a number of leaf 17). Induction and multiplication the shoot was an important early stages to multiply the seed of alfalfa in in vitro culture.

Pertumbuhan Tanaman Kedelai (Glycine Max L.) Hasil Radiasi Sinar Gamma Cesium-137

“…Gambar 3 menunjukkan bahwa untuk tinggi batang (cm) pada tanaman kedelai pada pemberian radiasi sinar Gamma Cesium-137 paling tepat untuk perlakuan dosisnya adalah B3 (75 mGy) persentase tinggi batang 3,7 cm dibandingkan dengan perlakuan dosis yang lainnya, Maka hasil pengukuran didapat bahwa semakin besar dosis yang diberikan, maka tinggi tanaman semakin menurun. Hal tersebut sesuai dengan pernyataan (Sajimin et al, 2015) (Harsanti & Yulidar, 2015) (Daeli et al, 2013 yaitu semakin tinggi dosis radiasi maka semakin menurun tinggi tanaman, penurunan tinggi tanaman terjadi karena radiasi dapat merusak kromosom tanaman, sehingga mengakibatkan terganggunya tanaman tersebut. Kemudian, Indriani, dkk.…”

Induction Shoots of Black Pomegranate (Punica granatum L.) Seed Explant Using Thidiazuron And Kinetin Hormone In Vitro

“…Shoot induction through epicotyl using the hormone BA 0.5 mg / L and TDZ 3.0 mg / L can produce shoots of 9.6 with a height of 14.6 mm and number of leaves was 17 in each culture with regular shoots [15]. Effect of TDZ and IAA hormones on shoot induction of Jatropa curcas L. Showed that TDZ 0.5 ppm + IAA 1.5 ppm and TDZ treatment 0.5 ppm + IAA 2 ppm were able to form shoots [16].…”