Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile

Joshi, Chetna; Khare, Sunil Kumar

doi:10.1590/s1516-89132012000100003

Cited by 14 publications

(3 citation statements)

References 19 publications

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“…As a result, the search for thermophilic microorganisms with potential for the synthesis of such enzymes has increased considerably in the last years, mainly 916 Substract and temperature… GOMES,A. F. S. et al filamentous fungi, which have been the most chiefly employed (JOSHI; KHARE, 2012;MORETTI et al, 2012). The fungus Aspergillus fumigatus is a widespread species commonly found in agricultural residues with numerous biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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“…Many researches have been focused on induction of cellulase and xylanase production by different monosaccharides, disaccharides, and carbohydrates used as sole carbon source [ 16 ]. Generally, the final concentration of the sugar tested as inducer corresponds to the 1% (w/v), demonstrating that this amount gives an inductive effect on both cellulases [ 17 , 18 ] and xylanases [ 19 , 20 ]. As regards the sophorose, although in most cases it was used as carbon source at final concentration of 1% (w/v) [ 21 – 23 ], its inductive effect on cellulase activity was also reported at lower concentration [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization ofArundo donaxSaccharification by (Hemi)cellulolytic Enzymes fromPleurotus ostreatus

Liguori

Ionata

Marcolongo

et al. 2015

BioMed Research International

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An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 33 factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.

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“…Thermophilic M. thermophilus is a wellknown mushroom compost fungus that produces lignocellulosedegrading enzymes. 19,20 Genome sequence analysis of M. thermophilus reveals the presence of three xylanase-encoding genes of GH11 (Table 1), which share 76−100% sequence identities to known proteins but low identities (36−48%) to each other. The mature proteins are predicted to be extracellular and have an estimated molecular mass of 23.3−29.2 kDa and an alkaline pI value of 8.04−8.71.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides

Bai²,

Huang³

et al. 2017

J. Agric. Food Chem.

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Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In the present study, four neutral, thermostable xylanases (MtXyn11A, MtXyn11At, MtXyn11B, and MtXyn11C) from compost fungus Mycothermus thermophilus CGMCC3.18119 were overexpressed in Pichia pastoris GS115 and used to produce xylooligosaccharides from beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0-6.5 and 65 °C) but varied in catalytic efficiency and cleaving actions. MtXyn11A, MtXyn11At, and MtXyn11C mainly produced xylobiose (59-62%), xylose (16-20%), and xylotriose (16-19%), while MtXyn11B released xylobiose (51%), xylotriose (32%), and xylose (12%) as the main products. When using the xylan hydrolysates of different xylanases as the carbon source, four probiotic Lactobacillus strains Lactobacillus brevis 1.2028, Lactobacillus rhamnosus GG, Lactobacillus casei BL23, and Lactobacillus plantarum WCSF1 were confirmed to use the xylooligosaccharides efficiently (83.8-98.2%), with L. brevis 1.2028 as the greatest.

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Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile

Cited by 14 publications

References 19 publications

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Optimization ofArundo donaxSaccharification by (Hemi)cellulolytic Enzymes fromPleurotus ostreatus

Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides

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