Optimization of<i>Arundo donax</i>Saccharification by (Hemi)cellulolytic Enzymes from<i>Pleurotus ostreatus</i>

Liguori, Rossana; Ionata, Elena; Marcolongo, Loredana; Vandenberghe, Luciana Porto de Souza; Cara, Francesco La; Faraco, Vincenza

doi:10.1155/2015/951871

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…, 4 g/L de extrato de levedura, 0,001 g/L de tiamina e 0,2 g/L de tartarato de amônio (ASLAM; ASGHER, 2011; PÉREZ; PIÑEROS-CASTRO; LOZANO, 2011;KARP et al, 2015;LIGUORI et al, 2015). Para a dosagem de ART e das enzimas, em cada experimento, a cada um dos frascos fermentados, foram adicionados 50 mL de água destilada e homogeneizados por 1 hora a 200 rpm a temperatura ambiente.…”

Section: Obtenção De Açúcares Redutores Totaisunclassified

Produção simultânea de hemi(celulases) e açúcares redutores por Pleurotus ostreatus utilizando resíduos de Alstroemeria sp.

Zamora¹,

Darío²

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This study is the firts in Brazil to assess a simultaneous production of hemi(cellulolytic) enzymes and reducing sugars (RS) from Alstroemeria sp. stems and leaves by the fungus growth Pleurotus ostreatus PLO6 using solid state fermentation (SSF). The researching is pioneer to characterize brazilian flower waste and, from chemical view point, to taking advatange this biomass to obtain ART, regarding its subsequent conversion into second generation bioethanol. The chemical composition of this kind of waste was: moisture 10,1%, ash 7,5%, cellulose 36,1%, hemicelluloses 14,7% e lignin 15,2%. The elemental analysis was: carbon 42,8%, nitrogen 1,1%, hydrogen 5,4% and sulfur 1,3%. Besides, the metal concentrations were: cupper 20,0 mg/kg, iron 256,8 mg/kg, zinc 133,4 mg/kg, lead 0,0 mg/kg e manganese 105,22 mg/kg. The researching first stage (called preliminary) verified the simultaneous production of hemi(cellulolytic) enzimes and RS designing univariate experiments, where the response variables were (hemi)cellulolytic enzymatic activities and RS concentration, and the factor chosen was time between 0 and 14 days; performing quantification in duplicate each 24 hours. At this stage, the basidiomycete was able to produce endoglucanase, exoglucanase, β-glucosidase, endoxylanase and β-xylosidase enzymes, with maximum activities of 10,3 U/g (day 4), 74,5 U/g (day 5), 26,3 U/g (day 6), 20,5 U/g (day 9) and 94,9 U/g (day 11), respectively and the RS highest concetration got was 3,3 g/L at 12th day of fermentation. After to check the simultaneous production of enzymes and RS, a second stage was performed (called screening), which assesed effects of temperature (T, o C), substrate concentration (SC) and time (t, days) on enzimatic synthesis and RS. This phase, was developed with a statistical factorial design 2 3 in triplicate, where, the factor nivels were 24 and 32 o C, 20 and 30 % de SC, and 8 and 15 days, and the response variables were the same used at preliminary phase. In this part, with a significance level of 0,05, the effect more significant on enzymes (except to β-xylosidase) and RS production was temperature; cellulolytic activities and RS were maximized during 24 o C, while hemicellulolytic enzymes were maximized at 32 o C. The last stage developed was RS optimization, it was performed based on central composite design (CCP), established acording to significance results obtained at screening phase. There, the highest production of RS predicted by the mathematical model was 4,35 g/L at 24,22 o C, 20,75% of SC and 8,94 days of fermentation; conditions very close to center point chosen (4,36 g/L at 24 o C, 20% of SC and 9 days of SSF) and the highest RS concentration achieved at screening stage (4,45 g/L with the same conditions of center point). The chemical composition of flower waste is inside the rank of cellulose, hemicelluloses and lignine, considered like alternative biomass to produce second generation bioethanol. According to content of carbon, nitrogen, cupper and manganese, Alstroemeria sp. waste were a su...

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Section: Obtenção De Açúcares Redutores Totaisunclassified

Produção simultânea de hemi(celulases) e açúcares redutores por Pleurotus ostreatus utilizando resíduos de Alstroemeria sp.

Zamora¹,

Darío²

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Simultaneous production of cellulases, hemicellulases, and reducing sugars by Pleurotus ostreatus growth in one-pot solid state fermentation using Alstroemeria sp. waste

Zamora

Silva

Varão

et al. 2021

Biomass Conv. Bioref.

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An overview on the recent developments in fungal cellulase production and their industrial applications

Singh

Bajar

Devi

et al. 2021

Bioresource Technology Reports

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Optimization ofArundo donaxSaccharification by (Hemi)cellulolytic Enzymes fromPleurotus ostreatus

Cited by 4 publications

References 52 publications

Produção simultânea de hemi(celulases) e açúcares redutores por Pleurotus ostreatus utilizando resíduos de Alstroemeria sp.

Produção simultânea de hemi(celulases) e açúcares redutores por Pleurotus ostreatus utilizando resíduos de Alstroemeria sp.

Simultaneous production of cellulases, hemicellulases, and reducing sugars by Pleurotus ostreatus growth in one-pot solid state fermentation using Alstroemeria sp. waste

An overview on the recent developments in fungal cellulase production and their industrial applications

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