Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes, Arthur Filipe Sousa; Santos, Bruna Silveira Lamanes dos; Franciscon, Emanuele Giuliani; Baffi, Milla Alves

doi:10.14393/bj-v32n4a2016-32935

Cited by 21 publications

(11 citation statements)

References 20 publications

(30 reference statements)

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“…In terms of optimal reaction pH, xylanases remained active at higher pH (pH 5) while at pH 3, their performance was reduced by 50% in comparison to the optimum conditions. These results are in agreement with those reported by Xiong who established the loss of solubility of xylanases at pH 3.5 (Xiong, Von Weymarn, Leisola, & Turunen, ) and with Gomes who reported an optimal activity at pH 4.5 and proved 60% loss of enzyme activity at pH 3 (Gomes, Santos, Fransciscon, & Baffi, ). In conclusion, pH is a crucial factor for enzyme activity and therefore it was not possible for xylanases to work at pH 3 where in turn the anthocyanins exhibited more stability.…”

Section: Resultssupporting

confidence: 92%

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Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Fernández-Aulis

Hérnández-Vázquez

Aguilar-Osorio

et al. 2019

Journal of Food Science

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Pigmented maize has been extensively studied due to its high anthocyanin content. This study has been focused mainly on kernel, although the whole plant of purple corn is a potential source of anthocyanins. First, general parameters of extraction (solvent system, solvent-to-solid ratio, number of extractions, and acid type) were established depending on the total anthocyanins content. Then, three extraction methods to access anthocyanins were compared: maceration extraction (ME), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE). Since the residual material still possessed an intense color, a further treatment was performed by application of enzymatic-assisted extraction (EAE). Three enzymatic cocktails (Xylanases, Celluclast, and Depol), pH, and temperature were evaluated to establish optimal reaction conditions. Subsequent analysis and identification of the anthocyanins obtained by four different extraction techniques were performed using HPLC and HPLC-mass spectrometry, respectively. The most efficient method was UAE using 20 min of ultrasound (100 W) preceded by sample treatment in the following conditions: ethanol/water/lactic acid mixture (80:19:1), two extractions, 1:10 solvent-to-solid ratio. As a result, anthocyanins from corn cob and corn husk were extracted at concentrations of 24.32 and 25.80 mg/gDW, respectively. No difference in the anthocyanins profile for samples extracted by three different methods was observed. However, an enhanced presence of cyanidin-3-(6''malonyl)glucoside was detected in the sample corresponding to the EAE method. Therefore, the Cahuacintle corn husk can be considered as a competitive source of anthocyanins with the available commercial sources.

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Section: Resultssupporting

confidence: 92%

“…Moreover, we found that the best temperature for the enzymatic reaction was 40 °C. Although the optimal activity reported for xylanases was at 60 °C (Gomes et al., ), obtained results indicated on anthocyanins degradation at 50 °C. Thus, to improve enzymatic activity, temperature was raised to 40 °C instead of 30 °C.…”

Section: Resultsmentioning

confidence: 96%

Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Fernández-Aulis

Hérnández-Vázquez

Aguilar-Osorio

et al. 2019

Journal of Food Science

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“…On the other hand, the xylanase produced by A. terreus NRRL260 recording 804 U. g -1 ds in the present work was better than that produced by A. terreus isolate grown on wheat bran through SSF recording 116 U. g -1 ds (Kaushik and Malik, 2016) and also better than that produced by A. fumigates strain SCB4 grown on a mixture of sugarcane bagasse and wheat bran (1:1) through SSF recording 574 U. g -1 ds (Gomes et al, 2016).…”

Section: Figcontrasting

confidence: 52%

Multi fungal cellulases produced on pretreated sugar cane bagasse and rice straw cooperative actions

Mahdy

Emara

El-Salam

et al. 2018

Journal of Environmental Sciences. Mansoura University

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Twenty eight pre-identified fungal isolates and strains were tested qualitatively for their cellulolytic activities, among which 12 superior producers were quantitatively tested. Three Aspergillus spp. namely A5, A6 and A7 were found to be the best cellulolytic fungi, while both Aspergillus terreus NRRL260 and Phanerochaete chrysosporium NRRL6361 were found to be the best xylanase producers. On solid state fermentation (SSF) using pretreated rice straw (RS), the Aspergillus spp. A5, A6 and A7 gave total cellulases (as FPase), exocellulases (as CMCase) and cellobiase (as β-glucosidase) activities of ≥ 16, 130 and 21U.g-1 ds, respectively. On the other hand, Aspergillus terreus NRRL260 showed maximum xylanases activity of 804 U.g-1 ds. The recovered crude enzymes forming the crude enzyme mixture (CEM) was concentrated (LCEM), ammonium sulfate fractionated (PPE1), were semi-purified by gel permeation on Sephadex G100 and finally lyophilized to powder form (PPE2). The resulting cellulases specific activities were 0.50, 0.68, 1.01 and 1.47 U.mg-1 , respectively. Those purification and concentration steps resulted in purification fold increase by 1.28, 2.08 and 2.78 for LCEM, PPE1 and PPE2, respectively. PPE2 achieved maximum degree of saccharification (DoS) at enzyme load ≥ 10 FPU, substrate concentration ≥ 5% and incubation period after 48 hr to be 85%, 87% and 88%, respectively.

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“…Xylanase activity was the highest at 55C (Figure 3) and pH 5.0 (Figure 4). According to other studies, the optimum pH and temperature for A. fumigatus growth ranged 3.0-6.0 and 55C-60C, respectively (Ang et al, 2013;Gomes et al, 2016).…”

Section: Optimisation Of Conditions For Xylanase Productionmentioning

confidence: 80%

Xylanase production by Aspergillus fumigatus UR01 by solid state fermentation of rice straw

Singhajutha¹

2020

Asian J Agric Biol

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Sugar generation is important for many industries, such as livestock feed and bioethanol production. Rice straw is an agricultural waste that serves as a good source of sugar. In this study, a xylanase-producing fungus was isolated form compost pile and identified as Aspergillus fumigatus based on the internal transcribing spacer sequence. Xylanase produced by this fungus was characterised. The optimal conditions for fungal xylanase activity were 55C and pH 5.0. Enzyme activity declined to 45.26% following incubation at 50C for 2 days. Rice straw was more suitable for xylanase production than other substrates. The filtrate of A. fumigatus grown on rice straw as a substrate was added to rice straw, and reducing sugar production was determined. The maximum reducing sugar production was achieved with incubation at 30C for 18 h. Xylanase produced by A. fumigatus can be used for rice straw pre-treatment to improve the nutrient content of livestock feed and increase bioethanol production.

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Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Cited by 21 publications

References 20 publications

Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Multi fungal cellulases produced on pretreated sugar cane bagasse and rice straw cooperative actions

Xylanase production by Aspergillus fumigatus UR01 by solid state fermentation of rice straw

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