Induction of tolerance to desiccation and to subzero temperatures in embryos of recalcitrant seeds of inga

Bonjovani, Marcio Roberto; Barbedo, Claudio José

doi:10.1590/2317-1545v36n41027

Cited by 12 publications

(9 citation statements)

References 16 publications

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“…An option for long-term storage of recalcitrant seed is through cryopreservation (Engelmann, 2011) that is a method of high cost that requires a high level of technology (Bonjovani & Barbedo, 2014).…”

Section: Discussionmentioning

confidence: 99%

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Shibata

Coelho²

2016

RBT

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Abstract:Araucaria angustifolia is a conifer native to Brazil and is an endangered species. Since this species seeds have a short period of viability, its vulnerability is higher. Thus the aim of this study was to evaluate the physiological quality of A. angustifolia seeds during the development and post-storage periods. For this, cones of A. angustifolia were collected from a natural population in Curitibanos, Santa Catarina, Brazil, in March, April, May and June 2012. The collected seeds were classified into developmental stages of cotyledonary, i, ii and iii according to the month of collection; a total of 10 cones were collected for each stage. Seeds were stored in a refrigerator for 60 and 120 days, and were submitted to a chamber germination test (25 °C-photoperiod 12 h). Additionally, seeds were tested for moisture content (105 °C for 24 hours), tetrazolium (0.1 % for 1 hour) and vigor (electric conductivity [75 mL distilled water at 25 °C], germination speed index, and shoot and root length). Our results showed that during seed development, moisture content decreased from the cotyledonary stage (66.54 %) to stage iii (49.69 %), and vigor increased in the last stage. During storage, moisture content at cotyledonary stage and stage i was stable. On the other hand, stored seeds exhibited a decrease in moisture content after 120 days at stages ii and iii. Physiological quality at the cotyledonary stage resulted in an increased germination rate of 86 % and 93 % after 60 and 120 days of storage, respectively; unlike stages ii and iii exhibited a decrease in seed viability and vigor after storage. Electrical conductivity was higher for fresh seeds at the cotyledonary stage, than for those stored for 60 and 120 days. However, in other stages, released leachate content after 120 days of storage, increased with the advance of the collection period. Germination speed index and shoot and root lengths after storage were highest for seeds at the cotyledonary stage and stage i; unlike stages ii and iii which had short root and shoot lengths during storage. Thus, the maintenance of seed moisture content during storage was variable and dependent on the period of collection. Furthermore, the physiological quality differed among earlier and later stages. Early collection favored seed physiological quality, and may be a strategy for better conservation of A. angustifolia seeds. Rev. Biol. Trop. 64 (2): 885-896. Epub 2016 June 01.Key words: recalcitrant seed, storage, seed development, gymnosperms, endangered plants.Seed development is a process that comprises a set of morphological, cellular, and biochemical/synthetic changes which commences with ovule fertilization to the moment in which the seed is mature (Bewley, Bradford, Hilhorst, & Nonogaki, 2013).Most seeds undergo a process of "maturation drying" with late stages of development, and these seeds are called orthodox (Roberts, 1973). Before or concomitant with maturation drying, seeds acquire desiccation tolerance, and can be dried to low water content (generally less ...

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Section: Discussionmentioning

confidence: 99%

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Shibata

Coelho²

2016

RBT

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“…Therefore, this species greatly interests studies aiming at the development of procedures to expand the storage potential of recalcitrant seeds. Researches have demonstrated that using specific techniques can extend the storage time of these seeds (Andréo et al, 2006;Faria et al, 2006;Bonjovani and Barbedo, 2008;Bonjovani and Barbedo, 2014;Parisi et al, 2016), but the knowledge on the physiology behind such improvement is still limited. A better comprehension of these processes could help to find new technologies that preserve the seeds for longer and that are also compatible with their preservation in germplasm banks.…”

Section: Introductionmentioning

confidence: 99%

Respiration and deterioration of Inga vera ssp. affinis embryos stored at different temperatures 1

Bonjovani

Barbedo

2019

J. Seed Sci.

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Inga vera ssp. affinis embryos at different maturity stages and with various water contents had their CO2 production and O2 consumption analyzed, and then compared to those of Phaseolus vulgaris L. seeds. The results showed that the lower the temperature, the lower the respiratory rates and the more intense the other oxidative processes of I. vera embryos. On the other hand, the same did not happen in P. vulgaris seeds. Oxidative reactions, including respiration, reduced the germinative capacity and compromised the preservation of the embryos and seeds. Oxidative reactions depended on the embryo maturity stage, its moisture level, and the surrounding temperature. Interestingly, the responses obtained for viability loss and changes in O2 consumption rates of I. vera embryos did not correspond to the expected for plant tissues, as verified in P. vulgaris seeds with similar water content.

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“…Studies on the metabolism of recalcitrant seeds have been performed by several authors (Barbedo & Bilia 1998;Song et al 2003;Berjak & Pammenter 2008;Caccere et al 2013;Pammenter & Berjak 2014;Walters 2015), including studies on ex situ conservation by storage (Pammenter et al 1994;Drew et al 2000;Li & Pritchard 2009;Pasquini et al 2012;Charloq et al 2013;Walters et al 2013;Bonjovani & Barbedo 2014;Liu et al 2014). Storage of recalcitrant seeds under desiccating conditions resulted in the initiation of subcellular damage, which may be repaired when seeds are set-out to germinate (Farrant et al 1989;Tarquis & Bradford 1992).…”

Section: Introductionmentioning

confidence: 99%

Establishment of post-harvest early-developmental categories for viability maintenance of Araucaria angustifolia seeds

Araldi

Coelho

2015

Acta Bot. Bras.

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Araucaria angustifolia seeds are recalcitrant, and their metabolism remains high during storage. This research aimed to describe the initiation of germination in A. angustifolia seeds during storage in order to standardize the assessment of physiological quality and to promote seed conservation. Seeds were collected from two populations and stored for 270 days in the natural laboratory environment and cold chamber. Seeds were classified according to four early developmental stages: I -mature seeds; II -seeds with elongation along the embryonic axis; III -beginning of root protrusion; IV -advanced germination stage, with seedling shoots. After categorization, physical and physiological quality was assessed. In freshly collected seeds, only category I was observed. At 270 days, approximately 40% of seeds were in category III in laboratory conditions, while the maintenance in a cold chamber delayed germinative metabolism. Viability tests showed that seeds in categories III and IV were more susceptible to damage caused by storage.In conclusion, the percentage of viable A. angustifolia seeds depends on the development stage after collection. Seeds that have reached early developmental category III should be prioritized for propagation, while those remaining in categories I and II should be longer stored with periodic assessment for reduction in physiological quality.

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Induction of tolerance to desiccation and to subzero temperatures in embryos of recalcitrant seeds of inga

Cited by 12 publications

References 16 publications

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Respiration and deterioration of Inga vera ssp. affinis embryos stored at different temperatures 1

Establishment of post-harvest early-developmental categories for viability maintenance of Araucaria angustifolia seeds

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