2014
DOI: 10.1590/2317-1545v36n41027
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Induction of tolerance to desiccation and to subzero temperatures in embryos of recalcitrant seeds of inga

Abstract: -Drying, widely used for storing orthodox seeds for prolonged periods, cannot be applied to recalcitrant seeds, which are sensitive to desiccation. Thus, inclusion of species with recalcitrant seeds, like inga, in reforestation programs or even for commercial use has been hindered by the lack of technology that would allow storage of these seeds. The remaining option, cryopreservation, is a method of high cost that requires a high level of technology. Knowledge of the processes involved in sensitivity to desic… Show more

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Cited by 12 publications
(8 citation statements)
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“…An option for long-term storage of recalcitrant seed is through cryopreservation (Engelmann, 2011) that is a method of high cost that requires a high level of technology (Bonjovani & Barbedo, 2014).…”
Section: Discussionmentioning
confidence: 99%
“…An option for long-term storage of recalcitrant seed is through cryopreservation (Engelmann, 2011) that is a method of high cost that requires a high level of technology (Bonjovani & Barbedo, 2014).…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, this species greatly interests studies aiming at the development of procedures to expand the storage potential of recalcitrant seeds. Researches have demonstrated that using specific techniques can extend the storage time of these seeds (Andréo et al, 2006;Faria et al, 2006;Bonjovani and Barbedo, 2008;Bonjovani and Barbedo, 2014;Parisi et al, 2016), but the knowledge on the physiology behind such improvement is still limited. A better comprehension of these processes could help to find new technologies that preserve the seeds for longer and that are also compatible with their preservation in germplasm banks.…”
Section: Introductionmentioning
confidence: 99%
“…Studies on the metabolism of recalcitrant seeds have been performed by several authors (Barbedo & Bilia 1998;Song et al 2003;Berjak & Pammenter 2008;Caccere et al 2013;Pammenter & Berjak 2014;Walters 2015), including studies on ex situ conservation by storage (Pammenter et al 1994;Drew et al 2000;Li & Pritchard 2009;Pasquini et al 2012;Charloq et al 2013;Walters et al 2013;Bonjovani & Barbedo 2014;Liu et al 2014). Storage of recalcitrant seeds under desiccating conditions resulted in the initiation of subcellular damage, which may be repaired when seeds are set-out to germinate (Farrant et al 1989;Tarquis & Bradford 1992).…”
Section: Introductionmentioning
confidence: 99%