Induction of Tetraploids for Breeding Hardy Ornamentals

Rose, J. B.; Kubba, J.; Tobutt, K. R.

doi:10.17660/actahortic.2001.560.14

Cited by 4 publications

(3 citation statements)

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“…Several authors [24,25,26] have reported different effects of antimitotic agents when different tissues are targeted or different treatment periods are used. The efficiency of colchicine was documented for variant induction in several crops, medicinal and flowering plants [27,28,29]. The survival factor after colchicine treatment depends on the concentration and duration of the treatment.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of polyploidy Vicia faba induced by colchicine

Nagat¹,

Kamla²,

Hoda³

2020

GSC Biol. and Pharm. Sci.

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To induce polyploidy in diploid control plant of Vicia faba, seeds were socked in various concentrations of colchicine (0.1, 0.2, 0.3 and 0.4 %) for 9 and18 h. Putative ploidy plants were confirmed by screening morphological characters, measuring DNA content via spectrophotometer and analyzing stomatal behavior. Polyploidy plants showed slower growth rate along with increased potency and thickened large leaves. Three types of polyploidy were achieved; triploid, tetraploid and mixoploid. In induced polyploid plants, the length and width of stomata were increased, while the index of stomata was declined, in comparison to the initial diploid plants. Treatment with 0.3 and 0.4 % colchicine for 9 and 18 h were efficiently induced polyploidy. The highest stomatal size (22.53 µm) and widest stomatal aperture (9.72 µm) and the lowest stomatal index (34.24) where obtained when 0.4 % of colchicine where applied at 18hr tested time with high significant differences at p<0.05 in comparison to dipliod plants. We recommend this method for induction of polyploids in other different crops and possibly will selected these tetraploid plants for further breeding purposes.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of polyploidy Vicia faba induced by colchicine

Nagat¹,

Kamla²,

Hoda³

2020

GSC Biol. and Pharm. Sci.

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“…In tissue culture such processes are convenient, easy to track and additionally its factor of being time neutral helps it to be executed anytime. For induction of variants the efficiency of colchicine was reported in several medicinal (Wang et al 2002) and flowering plants (Rose et al 2001) including gerbera (Tosca et al 1995;Han et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2011

Plant Cell Tiss Organ Cult

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To induce variation through chromosome doubling in Gerbera jamesonii Bolus cv. Sciella, twoweek-old in vitro grown shoots were treated with various concentrations of colchicine (0.01, 0.05, 0.10, 0.50 or 1% w/v) for 2, 4 or 8 h. Treated shoots were then cultured on Murashige and Skoog (MS) medium supplemented with 8.8 lM 6-benzyladenine (BA) and 155 lM adenine sulphate (ADS), and subsequently transferred to fresh MS medium containing 2.85 lM indole-3 acetic acid (IAA) for rooting. When shoots were treated with 0.1% colchicine for 8 h, 64% of recovered plantlets were tetraploid. Ploidy of plantlets was confirmed by flow cytometry, stomatal analysis, and morphological characters. Tetraploid plantlets displayed slower proliferation along with higher vigor and thickened broad leaves. Moreover, tetraploid plants developed larger flowers, longer stalks, and have improved vase-life, all contributing to higher ornamental value of gerbera.

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“…Study of the male fertility of the hybrids revealed very low pollen production and no germination of the pollen could be observed in any of the samples analysed. Rose et al (2001) also successfully crossed doubled B. globosa and B. davidii. The obtained F1 seedling had yellow flowers tinted with mauve.…”

Section: Interspecific Breedingmentioning

confidence: 99%

Introgression of yellow flower colour in Buddleja davidii by means of polyploidisation and interspecific hybridisation

Laere¹,

Huylenbroeck²,

Bockstaele³

2011

Hort. Sci. (Prague)

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To introduce yellow colour in the commercial Buddleja davidii (2n = 4x = 76) assortment, an interspecific breeding programme with B. globosa (2n = 2x = 38) was started. The first step was to perform chromosome doubling in B. globosa. Two of the obtained tetraploid B. globosa plants were subsequently used as male parent in interspecific crosses with the white flowering B. davidii cv. Nanhoensis Alba. In total 182 interspecific crosses were made and 18 F1 hybrids were obtained. Genome size measurements, chromosome counts and genomic in situ hybridisation (GISH) analysis proved the hybrid nature of most of the F1 hybrids. Plant morphology also expressed hybrid characteristics. F1 seedlings inherited the yellowish flower colour from B. globosa. As for many other woody ornamentals, the creation of hybrids through interspecific hybridisation along with polyploidisation offers new opportunities for breeding in Buddleja.

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Induction of Tetraploids for Breeding Hardy Ornamentals

Cited by 4 publications

References 0 publications

Phenotypic and molecular characterization of polyploidy Vicia faba induced by colchicine

Phenotypic and molecular characterization of polyploidy Vicia faba induced by colchicine

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

Introgression of yellow flower colour in Buddleja davidii by means of polyploidisation and interspecific hybridisation

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