Anther culture of Sorghum bicolor (L.) Moench I. Effect of panicle pretreatment, anther incubation temperature and 2,4-D concentration 15 Abstract. In a study of anther culture in Sorghum bicolor it was found that culture incubation temperature had a significant effect on yields of microspore-derived callus. A temperature of 33°C was superior to 25 °, 28 ° or 37 ° C. With fresh, unpretreated material, callus was produced only from anthers incubated at 33 ° C.Panicle pretreatment at 7 ° , 14 ° , 20 ° , 25 ° or 28 ° C either had no effect or significantly decreased callus yield.Comparison of culture media containing a range of concentrations of 2,4-dichlorophenoxyacetic acid (0-3.0 mg 1-1) showed that callus yield increased significantly with concentration up to 2.5 mg1-1 and then declined slightly. Although 1174 calluses were produced, subsequent differentiation was poor and only four albino plants were regenerated.
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