Induction of somatic embryogenesis as an example of stress-related plant reactions

Zavattieri, Maria Amely; Frederico, António Miguel; Lima, M.; Sabino, Rui; Arnholdt‐Schmitt, Birgit

doi:10.2225/vol13-issue1-fulltext-4

Cited by 169 publications

(132 citation statements)

References 28 publications

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“…A stress signal, the wounding, that accompanies excision of the tissue or the isolation of the cells is the first signal, perceived by cells destined to become somatic embryos in tissue cultures (36). the positive effect of abiotic stress to the process of somatic embryogenesis is recently reviewed and summarized by Zavattieri (49). Moreover many stress-related proteins are associated with somatic embryogenesis and differentially expressed between embryogenic and non-embryogenic cultures (12).…”

Section: Resultsmentioning

confidence: 99%

Hydroxyproline Rich Proteins in Salt Adapted Embryogenic Suspension Cultures ofDactylis GlomerataL.

Zagorchev

Odjakova

2011

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 99%

Hydroxyproline Rich Proteins in Salt Adapted Embryogenic Suspension Cultures ofDactylis GlomerataL.

Zagorchev

Odjakova

2011

Biotechnology & Biotechnological Equipment

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“…An elevated level of ROS imposes a real threat to cell integrity and functioning but on the other hand, ROS play an important role in the regulation of various physiological processes including growth, development, hormone signalling, defence reaction, and PCD (Mittler 2002;Mittler et al 2004;Rodrí-guez-Serrano et al 2012). Among others, the involvement of ROS as second messengers in somatic embryogenesis of many plants species has been revealed (reviewed by Zavattieri et al 2010). In the presented study, the evaluation of oxidative stress intensity and activity of antioxidative system revealed some interesting associations with ME initiated in triticale anther cultures.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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To verify the hypothesis that cell redox status regulates the process of microspore embryogenesis (ME), reactive oxygen species (ROS) generation and the activity of enzymatic and non-enzymatic antioxidants were analyzed in eight doubled haploid lines of triticale with significant differences in embryogenic potential. The analyses were performed in anthers excised from freshly cut tillers (control) and from low temperature (LT) pre-treated tillers (3 weeks at 4°C) in which ME has been initiated. Significant associations between ME effectiveness and the variables studied were found. In control cultures, high superoxide dismutase (SOD) activity appeared crucial for microspore viability. On the other hand, positive though non-linear correlation between ME effectiveness and H 2 O 2 generation, and negative correlation with catalase (CAT) activity suggest that some threshold level of H 2 O 2 is important for successful ME initiation. LT tillers pretreatment significantly increased H 2 O 2 accumulation, which had a negative effect on ME effectiveness. However, even high level of H 2 O 2 did not endanger cell viability as long as the cells exhibited high activity of ROS-decomposing enzymes (SOD, CAT and POX). The ability to sustain antioxidative enzyme activity under cold stress in the dark was another important requirement for high effectiveness of ME, allowing for the generation of the signal initiating microspore reprogramming and simultaneously protecting the cells from the toxic effects of ROS production. The role of antioxidative enzymes cannot be replaced even by high activity of non-enzymatic antioxidants. In conclusion, genetically controlled but environmentally modified cell tolerance to oxidative stress seems to play an important role in triticale ME.

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“…For SE, during initiation proembryos start developing, followed by maturation during which storage products accumulate (for a review see ref. 4). A variety of factors including but not limited to species, explant source, and developmental stage impact upon competence for SE.…”

mentioning

confidence: 99%

Transcriptome analysis indicates that GmAGAMOUS-Like 15 may enhance somatic embryogenesis by promoting a dedifferentiated state

Perry

Zheng

2016

Plant Signaling & Behavior

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Induction of somatic embryogenesis as an example of stress-related plant reactions

Cited by 169 publications

References 28 publications

Hydroxyproline Rich Proteins in Salt Adapted Embryogenic Suspension Cultures ofDactylis GlomerataL.

Hydroxyproline Rich Proteins in Salt Adapted Embryogenic Suspension Cultures ofDactylis GlomerataL.

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Transcriptome analysis indicates that GmAGAMOUS-Like 15 may enhance somatic embryogenesis by promoting a dedifferentiated state

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