Induction of oxidative stress and SOS response in Escherichia coli by vegetable extracts: the role of hydroperoxides and the synergistic effect of simultaneous treatment with cisplatinum

Манухов, И. В.; Kotova, V. Yu.; Mal’dov, D. G.; Il’ichev, A. V.; Bel’kov, A. P.; Завильгельский, Г Б

doi:10.1134/s0026261708050020

Cited by 17 publications

(9 citation statements)

References 10 publications

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“…Confidence intervals for protective activity value were calculated by the described method. [15,16] Determination of spontaneous and induced mutagenesis parameters Cells of E.coli MG1655 (pRecA-lux) strain were inoculated into 4 ml of the LB medium and stirred evenly. The induced culture was divided into four equal aliquots.…”

Section: Bioluminescent Testmentioning

confidence: 99%

“…[12,15] The culture of E. coli MG 1655 pRecA-lux was grown in LB broth. Bacteria were grown in liquid culture at 37 °C.…”

Section: Bioluminescent Testmentioning

confidence: 99%

“…[3,13,14] Our experimental approach implies using E. coli-based Lux-biosensors, responding to the activation of the SOS pathway with an increase in luminescence. [15] Through this approach, it became possible to identify the inhibitors, effectively, working not only in model cell-free systems, but also within bacterial cells. Shifts in spontaneous and induced mutation frequency of antibiotic resistance in E. coli, induced with substance-1, were determined by methods of classical genetics of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

Chistyakov¹,

Празднова²,

Kharchenko³

et al. 2016

Int J Biomed

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The aim of the present study was to investigate biological properties of the novel nitrobenzoxadiazole derivative 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan. Materials and Methods:We used a bioluminescent test based on a set of lux-biosensors, which are genetically modified E.coli strains able to react on different types of factors that can induce an SOS-response with light emission. The spontaneous and induced mutation frequencies of antibiotic resistance in E. coli were determined by methods of classical genetics of microorganisms.Results: 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan demonstrated inhibition of SOS-response in a biosensor model system and significantly reduced the frequency of spontaneous mutations and mutations induced by ciprofloxacin of antibiotic resistance.Conclusion: Based on our data, we can recommend using compound 1 as a starting point for the development of drugs that block mutagenesis associated with the emergence of antibiotic-resistant bacteria. (Int J Biomed. 2016;6(3):228-232.).

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Section: Bioluminescent Testmentioning

confidence: 99%

“…[12,15] The culture of E. coli MG 1655 pRecA-lux was grown in LB broth. Bacteria were grown in liquid culture at 37 °C.…”

Section: Bioluminescent Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

Chistyakov¹,

Празднова²,

Kharchenko³

et al. 2016

Int J Biomed

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“…Laboratory strains of Escherichia coli were transformed by methods of genetic engineering and molecular biology using developed recombinant plasmids with lux-genes of natural microorganisms, which allowed to obtain sensory bacterial strains with a high background level of luminescence, which showed a decrease in luminescence intensity in response to the toxic effects of environmental factors. Further works in this area allowed to receive strains with relatively low background levels of luminescence, which had cloned indicator promoters before luminescence genes that allow to assess the cell response to environmental factors by the degree of luminescence increase (Manukhov et al, 2008). It is possible to design such bacterial strains that specifically react to certain toxicants (active oxygen metabolites, mutagens and heavy metals) or directly demonstrate the response of the target cell to adverse environmental factors (oxidative stress, damage to nucleic acids, synthesis of chaperones or membrane components).…”

Section: Introductionmentioning

confidence: 99%

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Logachev¹,

Каримов²,

Дускаев³

et al. 2015

Asian J. of Animal Sciences

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Research objective is the assessment of toxicity level of ruminal fluid of cattle with use of indicator luminescent strains of bacteria developed on the basis of E. coli. According to the research results systemic toxic action of ruminal fluid with use of sensory bacterial strains with constitutive luminescence (E. coli K12 TG1 with clone luxCDABE genes Photobacterium leiognathi 54D10) were assessed. During the contact of native ruminal fluid with sensory luminescent bacteria E. coli dose-dependent inhibition of luminescence in the first seconds of contact was registered. However, by the second minute of contact quenching was up to 5.20±0.59% in baseline environment and decreased after two-time dilution procedure up to 102.56±4.83% with dilution 1:1024). After 30 min of incubation of mixture level of luminescence in the sample with native ruminal fluid reached the control level and over time it increased to 190.59±22.15% after 60 min of contact. Strain E. coli katG::lux, where the cloned promoter is responsible for the synthesis of catalase providing the neutralization of hydrogen peroxide demonstrated luminescence induction of 3.0 relative units at the 60th minute of contact. Study of genotoxic effects based on activation of repair proteins RecA/LexA using strain E. coli recA::lux, allowed to register luminescence inducing effects of the bacterial cells in contact with native rumen fluid.

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“…This allows visualization of the transcriptional responses without the need to perform enzyme assays or add luciferase substrates exogenously. E. coli MG1655 containing pRecAlux3 was used to test the genotoxicity of chemicals [22]. Although the genetically modified bacteria can be massively obtained through continuous passage, the instability of these bacteria limits their application in practice.…”

Section: Introductionmentioning

confidence: 99%

Development of a novel genetically modified bioluminescent-bacteria-based assay for detection of fluoroquinolones in animal-derived foods

et al. 2014

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Fluoroquinolones (FQNs) are broad-spectrum antibacterial agents widely used in animal husbandry and aquaculture. The residues and antimicrobial resistance of such antibiotics are a major public health concern. To realize multianalyte detection of FQN residues, a genetically modified bacterium, Escherichia coli pK12 harboring plasmid pRecAlux3, was constructed in this study to develop a bioluminescent-bacteria-based assay for the detection of FQNs in animal-derived foods. This assay was based on the principle of induction of an SOS response by FQNs via inducing the recA-promoter-fused luciferase reporter gene existing on the plasmid pRecAlux3. E. coli pK12 was able to recognize 11 FQNs: difloxacin, enrofloxacin, ciprofloxacin, sarafloxacin, norfloxacin, danofloxacin, ofloxacin, pefloxacin, lomefloxacin, marbofloxacin, and orbifloxacin. This method could be applied to 11 edible tissues, including milk, fish muscle, and the muscles, livers, and kidneys of cattle, chickens, and pigs, with a very simple and rapid sample extraction procedure using only phosphate-buffered saline. The limits of detection of the FQNs were between 12.5 and 100 μg kg(-1), all of which were lower than the maximum residue limits. Most of the recoveries of the FQNs were in the range from 60 to 120 %, and the interassay coefficients of variation were less than 30 %. This method, confirmed by high-performance liquid chromatography, is reliable and can be used as both a screening test and a semiquantitative assay, when the identity of a single type of FQN is known.

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Induction of oxidative stress and SOS response in Escherichia coli by vegetable extracts: the role of hydroperoxides and the synergistic effect of simultaneous treatment with cisplatinum

Cited by 17 publications

References 10 publications

7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Development of a novel genetically modified bioluminescent-bacteria-based assay for detection of fluoroquinolones in animal-derived foods

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