Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Logachev, Konstantin; Каримов, И. Ф.; Дускаев, Г. К.; Фролов, А Н; Tulebaev, S.; Zavyalov, O.A.

doi:10.3923/ajas.2015.248.253

Cited by 21 publications

(8 citation statements)

References 11 publications

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“…Using the initial sample, the glow level at the 30 th minute was recorded at 3,149±145 RLU, which is 32% of the control level (Figure 1). As previously described [11], this effect is primarily associated with acute toxicity, which is confirmed by the four-time difference of luminescence (577±36 RLU in the experiment vs. 2232±132 RLU in the control) at the first second of contact. Further two-fold dilution of rumen fluid led to the expected reduction of the toxic action with changing the shape of the glow kinetic curve.…”

Section: Resultssupporting

confidence: 83%

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

et al. 2020

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A way to assess the metabolic state of bacteria of rumen microflora cells is the registration of glow kinetics including recombinant strains, which is a reflection of stress states developing in the cell. The experiment evaluated the effect of rumen fluid in combination with probiotic strains and vanillin on the luminescence of a recombinant strain Escherichia coli MG1655 and digestibility of feed. The “in vitro” method with the rumen simulation technique (Rusitec) was used to determine the digestibility of the dry substance of feed. Using the initial sample, the glow level at the 30th minute was recorded at 3,149±145 RLU, which is 32% of the control level. In the presence of rumen fluid (at the concentration of 6.25%), there was observed an improved response by E.coli against Saccharomyces boulardii’s background (by 44%). The use of vanillin in rumen fluid increases the glow level of bacterial cells in the range of concentrations up to 50%, and in combination with probiotic strains, in the range of 25-50 %. In vitro conditions against the background of rumen fluid showed that vanillin in combination with probiotic strains Bifidobacterium longum and Bifidobacterium adolescentis / Lactobacillus acidophilus contributes to an increase in the digestibility of the dry substance of feed.

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Section: Resultssupporting

confidence: 83%

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

et al. 2020

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“…On the other hand, the biosensor based on B. subtilis EG168-1 also reacted on the control samples with a drop in luminescence up to 15%, however, samples of the I and II variants led to quenching by 65% and 60%, respectively. A similar effect was registered earlier [6], it was established that after contact of native ruminal fluid with bioluminescent E. coli strain, a dose-dependent inhibition of luminescence was registeredon the first seconds of contact.…”

Section: Resultssupporting

confidence: 83%

“…At the same time, the need for studying the influence of these extracts directly on the microbiome, changes in its biochemical parameters may be of interest when studying intercellular chemical communication in bacteria in the rumen. The previously obtained results confirm the promising nature of these studies [6,7].…”

Section: Introductionsupporting

confidence: 84%

Ecology of Ruminal Microorganisms Under the Influence of Quercus Cortex Extract

Дускаев¹

2019

GEOMATE

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The study was performed for assessing the elemental status and the bacterial composition of the rumen fluid microflora in cattle after incubation in vitro with quercus cortex extract and exogenous enzymes. The mixture of alfalfa hay, sudan grass and concentrates (60:40) was used as the substrate. The results showed that in the protozoa a decrease in such elements as p, na, ca (p≤0.05) was observed when substrate with enzyme was added. The level of essential elements in bacteria three hours after the incubation together with the enzyme changed similar to the protozoa, except for copper, the content of which increased two times (p≤0.05). The introduction of quercus cortex extract into the substrate with the enzyme preparation three hours after the incubation promoted a significant increase in iron, manganese and cobalt content in the protozoa (p≤0.05). Unlike other variants, incubation of the substrate with the enzyme together with the extract increased the number of representatives of the taxa firmicutes by 13.2%, bacteroidetesby 13.4% (p≤0.05) against the background of a slight decrease in proteobacteria, saccharibacteria and fibrobacteres. An increase was observed in the number of microorganisms belonging to the taxa clostridia (by 23.7%; p≤0.05) and bacteroidia (by 13.4%; p≤0.05). The biosensor based on b.subtilis on samples with the extract reacted by quenching the luminescence by 60% on the 5th minutes and by 32% on the 60th minute contact. Thus, the chemical composition of oak bark extract significantly influenced the elemental profile and the bacterial composition of cattle rumen microorganisms.

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“…The formation of a complex of ruminal fluid with coumarin and coniferyl alcohol resulted in a decrease in the expression of the sensory promoter and a decrease in the quantum yield of E. coli strain JLD271 pAL103. This can probably be explained by the dose-dependent inhibition of luminescence, as shown by the results of previous studies [18] on the assessment of toxicity degree in cattle ruminal fluid, using indicator luminescent strains designed on the basis of E. coli.…”

Section: Discussionmentioning

confidence: 98%

Assessment of N-(3-Oxohexanoyl)-L-Homoserine Lactone and Vegetable Molecules in Vitro

Каримов¹

2019

GEOMATE

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The ban on the use of antibiotics in the feed of farm animals has promoted an active search for new substances with similar properties. Particular attention of researchers has been devoted to the search for these substances among medicinal plants. The effect of substances of vegetable origin, such as coumarin and coniferyl alcohol which both form part of oak bark, on the activity of acyl homoserine lactone (AHL) molecules has been investigated. Reporter luminescent test systems were used to quantify the stress effects using bioluminescent analysis. It was found that the absorption spectrum of coumarin is characterised by the presence of four peaks at 205, 215, 275, 310 nm. The interaction with coniferyl alcohol was characterised by a shift in the absorption maxima (250 and 265 nm). The introduction of a molecule of AHL into the system led to the disappearance of the peak at 250 nm and a significant decrease in the absorption at 265 nm. The formation of a complex of ruminal fluid and coumarin led to a decrease in the expression efficiency of the sensory promoter luxI to a level of 476626 RLU using the same concentration of GSL (homoserine lactone). The combination of ruminal fluid and coniferyl alcohol led to a decrease in the quantum yield of the E. coli strain to the level of 345896 RLU following the use of GSL at a concentration of 10 -4 M. The use of natural plant components (synthetic analogues) enhances the level of inhibition of quorum sensing (QS) system activity by the inactivation of regulatory molecules.

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Study of Intercellular Interaction of Ruminal Microorganisms of Beef Cattle

Cited by 21 publications

References 11 publications

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

Ecology of Ruminal Microorganisms Under the Influence of Quercus Cortex Extract

Assessment of N-(3-Oxohexanoyl)-L-Homoserine Lactone and Vegetable Molecules in Vitro

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