7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

Abstract

The aim of the present study was to investigate biological properties of the novel nitrobenzoxadiazole derivative 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan. Materials and Methods:We used a bioluminescent test based on a set of lux-biosensors, which are genetically modified E.coli strains able to react on different types of factors that can induce an SOS-response with light emission. The spontaneous and induced mutation frequencies of antibiotic resistance in E. coli were determined by methods of classical genetics of microorganisms.Results: 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan demonstrated inhibition of SOS-response in a biosensor model system and significantly reduced the frequency of spontaneous mutations and mutations induced by ciprofloxacin of antibiotic resistance.Conclusion: Based on our data, we can recommend using compound 1 as a starting point for the development of drugs that block mutagenesis associated with the emergence of antibiotic-resistant bacteria. (Int J Biomed. 2016;6(3):228-232.).

The Use of Biosensors to Explore the Potential of Probiotic Strains to Reduce the SOS Response and Mutagenesis in Bacteria

“…It is shown that SOS-deficient strains adapt more slowly to antibiotics [ 3 ]. Furthermore, as has been shown in our studies [ 4 ], activation of the SOS response by one therapeutic agent can lead to acquiring resistance to another.…”

“…Previously, we used this method to estimate the DNA-protective and antioxidant activity of bacterial fermentates [ 14 ]. Additionally, the SOS-inhibitory activity of a novel synthetic compound, nitrobenzoxadiazole derivative, 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan, was shown in one of our works [ 4 ].…”