The Use of Biosensors to Explore the Potential of Probiotic Strains to Reduce the SOS Response and Mutagenesis in Bacteria

Chistyakov, V. A.; Празднова, Е. В.; Mazanko, Maria S.; Bren, Anzhelica B.

doi:10.3390/bios8010025

Cited by 14 publications

(18 citation statements)

References 33 publications

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“…Whole-cell biosensors are widely used for assessing compounds' toxicity and ecological control [34,35]. Gram-positive and Gram-negative bacteria can have different sensitivities to the same toxicant.…”

Section: Discussionmentioning

confidence: 99%

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

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Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H2O2. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.

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Section: Discussionmentioning

confidence: 99%

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

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“…Our studies demonstrated that sporeforming probiotic organisms, obtained through solid phase fermentation, have antimutagenetic and antioxidative effects, as well as increase chicken mass and egg-laying ability [12,22,23].…”

Section: Methods Detailsmentioning

confidence: 99%

Method of preparation, visualization and ultrastructural analysis of a formulation of probiotic Bacillus subtilis KATMIRA1933 produced by solid-phase fermentation

Федоренко

Chistyakov

et al. 2019

MethodsX

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“…Lactobacilli are widely used in medicine and veterinary as probiotic bacteria (Acurcio et al, 2014;Ruzicka et al, 2016 Chistyakov et al, 2018;Mekadim et al, 2018). In addition to lactobacilli, bifidobacteria are actively used as probiotics, and they have similar properties (Saez-Lara et al, 2015;Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Assessing Antioxidant and Prooxidant Properties of Lactobacilli and Enterococci Strains by Using Orac and Lux-Biosensor Methods

Mazanko¹,

Chistyakov²,

Aleshukina³

et al. 2020

PTQ

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Lactobacilli are widely used in medicine as probiotic bacteria. Lactobacilli are considered one of the most important types of intestinal microbes. These bacteria have an antioxidant, gene protective effect on the immune and nervous systems of the host. But some strains of enterococci can be pathogenic microorganisms and cause diseases such as urinary tract infections, bacteremia, infections at the surgical sites, bloodstream infections, diarrhea. The need to study the beneficial and harmful properties of bacteria for humans determines the relevance of the study. The antioxidant and prooxidant properties of 11 Enterococcus strains and 7 metabolites of Lactobacillus strains were analyzed using an oxygen radical absorption capacity (ORAC) analysis and a lux biosensor test. Bacteria were incubated in cow's milk. Unfermented milk was used as a control. A study showed that milk fermented with enterococci did not have significant differences in antioxidant ability compared to control. In contrast, almost all lactobacilli increased the antioxidant ability of sour milk compared to unfermented milk. That is, the metabolites of Lactobacillus strains have demonstrated strong antioxidant properties even at low concentrations. The metabolites of Enterococcus strains possessed prooxidant properties. They enhanced the action of other prooxidants, such as paraquat, hydrogen peroxide, dioxidine, and showed a synergistic effect. The Lux biosensor test, used to evaluate the effect of substances on living cells with a complex metabolism, was more informative than the ORAC analysis, which allowed to evaluate the antioxidant and prooxidant properties of probiotic bacteria metabolites. The study revealed not only the influence of the direct effect of the test substance on the target molecule, but also the effect of indirect action by interfering with other biochemical processes of a living cell, which confirmed the need to use the biosensor lux test for further work when choosing strains of probiotic bacteria.

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The Use of Biosensors to Explore the Potential of Probiotic Strains to Reduce the SOS Response and Mutagenesis in Bacteria

Cited by 14 publications

References 33 publications

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Method of preparation, visualization and ultrastructural analysis of a formulation of probiotic Bacillus subtilis KATMIRA1933 produced by solid-phase fermentation

Assessing Antioxidant and Prooxidant Properties of Lactobacilli and Enterococci Strains by Using Orac and Lux-Biosensor Methods

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